Abstract
A method for determination of five kinds of ginkgolic acids in ginkgo seeds, C15:1, C17:2, C15:0, C17:1 using HPLC-MS was established. Isopropanol was employed as the extraction solvent and a component of the mobile phase. The isopropanol extract can be injected into the HPLC-MS apparatus directly and the lipid removal process with silica gel column chromatography can be saved. The limits of determination and quantification of this method are 0.977 and 0.293 mg kg−1 respectively. The coefficient of variation and the recovery of the determination method are 3.3 and 102.29% respectively. It has good linearity within the range of 0.0008–0.1 mg mL−1. This paper provides a simple and convenient method for the determination of ginkgolic acids in ginkgo seeds with the presence of a large amount of lipids.
Yanying Hu, Guojuan Sun—These two authors contributed to the work equally and are treated as co-first authors.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Koch E, Jaggy H, Chatterjee SS (2000) Evidence for immunotoxic effects of crude Ginkgo biloba L. leaf extracts using the popliteal lymph node assay in the mouse. Int J Immunopharmacol 22(3):229–236
Baron-Ruppert G, Luepke NP (2001) Evidence for toxic effects of alkylphenols from Ginkgo biloba in the hen’s egg test (HET). Phytomed Int J Phytotherapy Phytopharmacol 8(2):133–138. doi:10.1078/0944-7113-00022
Hecker H, Johannisson R, Koch E, Siegers CP (2002) In vitro evaluation of the cytotoxic potential of alkylphenols from Ginkgo biloba L. Toxicology 177(2–3):167–177
Kong YJ, Li CK, Wang PP, Song JY (2010) Study on separation of ginkgolic acids by resh adsorption. J Liaoning Univ Nat Sci Ed 37(01):57–60
Ndjoko K, Wolfender JL, Hostettmann K (2000) Determination of trace amounts of ginkgolic acids in Ginkgo biloba L. leaf extracts and phytopharmaceuticals by liquid chromatography-electrospray mass spectrometry. J Chromatogr B 744(2):249–255
Yin XL (2003) Study on separation technology of ginkgolic acids from the sarcotesta of Ginkgo biloba L. D, Jiangsu University
Gao ZS, Xian QM, Han XD, Pan XP, Feng JF, Yu HX (2008) Determination of nonylphenol in cells by pre-column derivatization and gas chromatography coupled with mass spectrometry. Environ Chem 27(6):819–822
Yang LQ, Wu XY, Chen J (2004) HPLC determination of ginkgolic acids in fruits of Ginkgo biloba. Chin J Pharm Anal 24(6):636–639
Chen WY, Wang CZ, Gao CX, Zhang SF, Zheng GY (2006) The study on the content of flavonoids and the chemical composition of lipids in seeds of Ginkgo biloba L. Biomass Chem Eng 40(6):6–8
Itokawa H, Totsuka N, Nakahara K, Takeya K, Lepoittevin JP, Asakawa Y (1987) Antitumor principles from Ginkgo biloba L. Chem Pharm Bull 35(7):3016–3020
Gouvea DR, Meloni F, Ribeiro Ade B, Lopes JL, Lopes NP (2012) A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicao) leaves: application to chemical variability evaluation. Anal Chim Acta 748:28–36. doi:10.1016/j.aca.2012.08.027
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Hu, Y., Sun, G., Zhang, H., Zhang, L., Zhang, T., Dai, Y. (2018). Determination of Ginkgolic Acids in Ginkgo Seeds Using HPLC-MS in the Presence of Lipids. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_45
Download citation
DOI: https://doi.org/10.1007/978-981-10-4801-2_45
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-4800-5
Online ISBN: 978-981-10-4801-2
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)