Abstract
In this work, the fermentation medium for γ-Aminobutyric acid (GABA) production by Enterococcus raffinosus TCCC11660 in shaken flasks was studied by the Plackett-Burman design. In the preliminary single-factor experiments, sucrose, pyridoxal 5′-phosphate (PLP) and glycine were found to have significant effects on GABA production at the suitable concentration ranges. Subsequently, the screened factors were optimized using Box-Behnken design of response surface methodology. The results showed that the optimal key medium compositions for GABA production by E. raffinosus TCCC11660 were 8.9 g/L citric acid, 0.82 g/L glycine and 0.21 mmol/L PLP, respectively. By fermentation medium optimization, GABA production reached 77.5 g/L, which was enhanced by 103.9% compared to the initial medium.
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References
Cho YR, Chang JY, Chang HC (2007) Production of gamma-aminobutyric acid (GABA) by Lactobacillus buchneri isolated from kimchi and its neuroprotective effect on neuronal cells. J Microbiol Biotechnol 17:104–109
Sandmeier E, Hale TI, Christen P (1994) Multiple evolutionary origin of pyridoxal-5′-phosphate-dependent amino acid decarboxylases. Eur J Biochem 221:997–1002
Trobacher CP, Zarei A, Liu J et al (2013) Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit. BMC Plant Biol 13:144
Ding JZ, Yang TW, Feng H et al (2016) Enhancing contents of gamma-aminobutyric Acid (GABA) and other micronutrients in dehulled rice during germination under normoxic and hypoxic conditions. J Agric Food Chem 64:1094–1102
Park KB, Oh SH (2007) Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3. Bioresour Technol 98:312–319
Gao Q, Duan Q, Wang DP, Zhang YZ et al (2013) Separation and purification of γ-aminobutyric acid from fermentation broth by flocculation and chromatographic methodologies. J Agric Food Chem 61:1914–1919
Shi XF, Chang CY, Ma SX et al (2016) Efficient bioconversion of L-glutamate to γ-aminobutyric acid by Lactobacillus brevis resting cells. J Ind Microbiol Biotechnol. doi:10.1007/s10295-016-1777-z
Plokhov AY, Gusyatiner MM, Yampolskaya TA et al (2000) Preparation of γ-aminobutyric acid using E. coli cells with high activity of glutamate decarboxylase. Appl Biochem Biotechnol 88:257–265
Komatsuzaki N, Shima J, Kawamoto S et al (2005) Production of γ-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 22(6):497–504
Choi SI, Lee JW, Park SM et al (2006) Improvement of gamma-aminobutyric acid (GABA) production using cell entrapment of Lactobacillus brevis GABA 057. J Microbiol Biotechnol 16:562–568
Li H, Gao DD, Cao YS et al (2008) A high γ-aminobutyric acid-producing ability: Lactobacillus brevis isolated from Chinese traditional paocai. Ann Microbiol 58:649–653
Siragusa S, Angelis M, Cagno R et al (2007) Synthesis of γ-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl Environ Microbiol 73:7283–7290
Park KB, Oh SH (2006) Isolation and characterization of Lactobacillus buchneri strains with high gamma-aminobutyric acid producing capacity from naturally aged cheese. Food Sci Biotechnol 15:86–90
Nomura M, Kimoto H, Someya Y et al (1998) Production of gamma-aminobutyric acid by cheese starters during cheese ripening. J Dairy Sci 81:1486–1491
Skeie S, Ardo Y (2000) Influence from raw milk flora on cheese ripening studied by different treatments of milk to model cheese. LWT Food Sci Technol 33:499–505
Li H, Qiu T, Gao D (2010) Medium optimization for production of gamma-aminobutyric acid by Lactobacillus brevis NCL912. Amino Acids 38:1439–1445
Izmirlioglua G, Demircia A (2016) Strain selection and medium optimization for glucoamylase production from industrial potato waste by Aspergillus niger. J Sci Food Agric 96:2788–2795
Triguerosa DEG, Fioresea ML, Kroumov AD et al (2016) Medium optimization and kinetics modeling for the fermentation of hydrolyzed cheese whey permeate as a substrate for Saccharomyces cerevisiae var. boulardii. Biochem Eng J 110:71–83
Chen HM, Gao Q, Su Z et al (2012) Screening, identification and flask fermentation optimization of a high-yield γ-aminobutyric acid Enterococcus raffinosus strain. Microbiol China 39:1642–1652 (in Chinese)
Shi XF, Zheng B, Chang CY et al (2015) Enzymatic bioconversion for γ-aminobutyric acid by Lactobacillus brevis CGMCC No. 3414 resting cells. Lect Notes Electr Eng 333:609–617
Rastogi NK, Rashmi KR (1999) Optimisation of enzymatic liquefaction of mango pulp by response surface methodology. Eur Food Res Technol 209:57–62
Acknowledgements
This work was financially supported by the National Basic Research Program (973 Program) of China (2013CB734004), the National Natural Science Foundation of China (31370075, 31471725 & 61603273), and the Youth Innovation Fund of Tianjin University of Science & Technology of China (2014CXLG28).
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Chang, CY., Ma, SX., Zhang, J., Gao, Q. (2018). Medium Optimization for γ-Aminobutyric Acid Production by Response Surface Methodology. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_41
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