Abstract
Phage adsorption to specific receptors on cell surface of host bacteria is the first step in the infection process, and phage receptor mutation is the most common way for bacteria to resist phage infection. In this study, the transposon Tn5G insertion mutant library of Pseudomonas aeruginosa PAK was constructed, and the mutants resistant to phages O1, O2, K1, K2, K3, and C10, respectively, were screened. Totally, 23 phage-resistant mutants were selected with the significantly decreased adsorption rates, showing the inactivated genes may be associated with the phage receptor synthetic pathways. With inverse PCR, 11 different genes were identified and eight of them are involved in lipopolysaccharide (LPS) synthesis, including gene wbpO, wbpV, wbpR, wbpT, wbpL, wzy, PA5001, and PA5004. The remaining three involved genes included bifA responsible for the hydrolysis of cyclic diguanylate in the folate synthesis pathway, PA0583 encoding the 2-amino-4-hydroxy-6-hydroxymethyldi hydropteridine diphosphokinase, and gene PA5181 encoding the molybdenum oxidoreductase. The inactivated genes were verified by the complement experiments. In conclusion, the results provide the genetic information about the phage receptors and will help to select candidate phages in the potential phage applications.
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This work was partly supported by The National Natural Science Foundation of China (grant 31370205 and 30970114).
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You, J., Cui, X., Sun, L., Yang, X., Yang, H. (2018). Identification of Genes Encoding Receptors for Six Pseudomonas aeruginosa Phages. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_4
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