Abstract
To find the relationship between CoQ10 synthesis and cytochrome accumulation in R. sphaeroides 2.4.1, we constructed two mutants (△prrA and △ppsR). ppsR mutation apparently activated cytochrome synthesis under aerobic cultivation, while prrA defection obviously inhibited the synthesis of cytochrome. Aerobic CoQ10 fermentation revealed △prrA growth was repressed after culturing for 24 h and the final biomass accumulated was reduced by 15.6%. Simultaneously, the final CoQ10 yield was reduced to 36 ± 0.58 mg/L which was only 85.7% as that of the WT. Also, both the bacterial growth and the CoQ10 yield of △ppsR were decreased. In the aspect of CoQ10 content, the result showed different phenomena that CoQ10 content for △prrA was increased, while for △ppsR was reduced. The result further supposed cytochrome accumulation competed with the CoQ10 synthesis in common precursor.
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Li, P., Gao, D., Gao, J., Liu, H., Qi, Z. (2018). Relationship Between Coenzyme Q10 Synthesis and Cytochrome Accumulation in Rhodobacter sphaeroides 2.4.1. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_38
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