Abstract
To explore the function of probiotic bacteria in puer tea pile fermentation which was implemented by a symbiosis of many fermenting microbes and enzymes, the species, abundances and dynamics of probiotic bacteria were quantitatively investigated. Using the real-time quantitative PCR (qPCR) primers specifically targeting different genera bacteria, the results showed that Enterococcus spp., Lactococcus spp., Lactobacillus group and Bacillus spp. were all found at 0, 15, 30, and 45 days during the fermentation, but Bifidobacterium spp. was not involved throughout the fermentation. Furthermore, among these four genera, Lactococcus spp. was the predominant probiotic with population of 1.68 × 109–2.78 × 109 copy g−1 in the fermentation, followed by Enterococcus spp. harboring 3.43 × 104–6.25 × 105 copy g−1, and Lactobacillus group and Bacillus spp. had 4.26 × 103–5.17 × 104 and 2.21 × 103–2.31 × 105 copy g−1, respectively. In addition, the dynamics of four genera probiotic bacteria and universal bacteria were explored to show completely different and complex change trends with no significant correlation with pH of tea and each other. Population dynamic studies of probiotic bacteria revealed their ecological feature and importance for puer tea pile fermentation, and also facilitated further exploration of probiotic resources and health benefits of puer tea.
Keywords
Shuang Li and Zhongyuan Li: Co-first authors.
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Chen Y, Liu B, Chang Y (2010) Bioactivities and sensory evaluation of Pu-erh teas made from three tea leaves in an improved pile fermentation process. J Biosci Bioeng 109:557–563
Lyu C, Chen C, Ge F, Liu D, Zhao S, Chen D (2013) A preliminary metagenomic study of puer tea during pile fermentation. J Sci Food Agr 93:165–3174
Anderson R, Polansky M (2002) Tea enhances insulin activity. J Agric Food Chem 50:7182–7186
Yang D, Hwang L (2006) Study on the conversion of three natural statins from lactone forms to their corresponding hydroxy acid forms and their determination in Pu-Erh tea. J Chromatogr A 30:1–2
Hayakawa S, Kimura T, Saeki K, Koyama Y, Aoyagi Y, Noro T, Nakamura Y, Isemura M (2001) Apoptosis-inducing activity of high molecular weight fractions of tea extracts. Biosci Biotech Biochem 65:459–462
Wen Q, Liu S (1991) Variation of the microorganism groups during the pile-fermentation of dark green tea. J Tea Sci 11:10–16
Zhou H, Li J, Zhao L, Han J, Yang X, Yang W, Wu X (2004) Study on main microbes on quality formation of Yunnan puer tea during pile-fermentation process. J Tea Sci 24:212–218
Pace NR (1997) A molecular view of microbial diversity and the biosphere. Science 276:734–740
Raspor P, Goranovic D (2008) Biotechnological applications of acetic acid bacteria. Crit Rev Biotechnol 28:101–124
Monhammad FG, Alireza T (2007) Isolation and characterization of polyphenol oxidase and peroxidase-producing Bacillus strains from fully fermented tea (Camellia sinensis). World J Microbiol Biotechnol 23:1327–1332
Klayraung S, Okonogi S (2009) Antibacterial and antioxidant activities of acid and bile resistant strains of Lactobacillus fermentum isolated from miang. Braz J Microbiol 40:757–766
Cho K, Math RK, Islam SM, Lim WJ, Hong SY, Kim JM, Yun MG, Cho JJ, Yun H (2009) Novel multiplex PCR for the detection of lactic acid bacteria during kimchi fermentation. Mol Cell Probes 232:90–94
Ganesan B, Weimer B, Pinzon J, Dao KN, Rompato G, Brothersen C, McMahon D (2014) Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria. J Appl Microbiol 116:1642–1656
Brady SF (2007) Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules. Nat Protoc 2:1297–1305
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386
Matijašić B, Obermajer T, Rogelj I (2010) Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by real-time PCR. Food Control 21:419–425
Li Z, Zhao H, Yang P, Zhao J, Huang H, Xue X, Zhang X, Diao Q, Yao B (2013) Comparative quantitative analysis of gene expression profiles of glycoside hydrolase family 10 xylanases in the sheep rumen during a feeding cycle. Appl Environ Microbiol 79:1212–1220
Lubbs DC, Vester BM, Fastinger ND, Swanson KS (2009) Dietary protein concentration affects intestinal microbiota of adult cats: a study using DGGE and qPCR to evaluate differences in microbial populations in the feline gastrointestinal tract. J Anim Physiol Anim Nutr (Berl) 93:113–121
Rinttila T, Kassinen A, Malinen E, Krogius L, Palva A (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97:1166–1177
Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP (2001) Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67:2578–2585
Heilig HG, Zoetendal EG, Vaughan EE, Marteau P, Akkermans AD, de Vos WM (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68:114–123
Klijn N, Weerkamp AH, de Vos WM (1995) Detection and characterization of lactose-utilizing Lactococcus spp. in natural ecosystems. Appl Environ Microbiol 61:788–792
Han GQ, Xiang ZT, Yu B, Chen DW, Qi HW, Mao XB, Chen H, Mao Q, Huang ZQ (2012) Effects of different starch sources on Bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets. Mol Biol Rep 39:1869–1876
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Li, S., Li, Z., Feng, C., Zhang, T. (2018). Comparative Quantitative Analysis of Probiotic Bacteria During Puer Tea Pile Fermentation. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_34
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DOI: https://doi.org/10.1007/978-981-10-4801-2_34
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