Abstract
Epothilones are a class of secondary metabolites mainly generated by Sorangium cellulosum of the suborder myxobacteria, there is a great potential applications in the development and application aspects of anticancer drugs. It has been reported to a variety of methods to improve the yield of epothilones, including strains selection, fermentation medium and process optimization, the application of fermentation adsorbent and heterologous expression, etc. This paper firstly investigates the influence of adding a variety of small organic molecules on the fermentation yield of epothilones, we were surprised to find that the methanol had significant promoting effect on the increase of the yield of epothilone A and B more than 10%. We furtherly analyzed the effect of methanol on the Sorangium cellulosum of cell growth, pH value and reducing sugar and other primary metabolites, we also investigated the relative expression changes of several key enzymes in the process of synthesis epothilones. The results showed that methanol on epothilone yield improvement is mainly effect on secondary metabolism, has nothing to do with the primary metabolism. We had enhanced the yield of epothilones to the range of 15–20% by one time adding volume ratio of 0.05% methanol to fermentation medium before inoculate, the optimal method is from the experiments on adding amount of methanol, adding time and adding methods.
Keywords
Lin-hao Zhang and Lin Zhao have contribute equally to this paper.
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Acknowledgements
The work was financially supported by Shandong Provincial Key Research and Development Program (No. 2015ZDZX05001, 2015ZDXX0502B01, 2015ZDXX0403B01, 2016GGB01409), National Natural Science Foundation (No. 31501396), Shandong Provincial Natural Science Foundation (No. ZR2012CQ027).
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Zhang, Lh., Zhao, L., Sun, X., Liu, Xl. (2018). Analysis and Application of the Promotion Action of Methanol on Sorangium cellulosum Produce Epothilones. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_32
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DOI: https://doi.org/10.1007/978-981-10-4801-2_32
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