Abstract
In order to discuss the effect of yeast extract on ε-poly-L-lysine (ε-PL) fermentation, the effect of yeast extract which are from different factories on fermentation of Streptomyces diastatochromogenes 6#-7 was performed. The result shows that there is a significant difference when adding yeast extract which are from different factories (p < 0.05), yet no significant difference in yeast extract which are from different production date of same factory (p > 0.05).In addition, the effect of different concentration of yeast extract in medium on fermentation of strain 6#-7 was investigated. When the concentration of yeast extract is 15 g/L, the ε-PL yield of strain 6#-7 can be up to 1.34 g/L and has a 6-fold enhancement compared to the group without yeast extract. The study of the key enzyme activities indicated that the improvement of ε-PL may be due to the concentration of yeast extract and the growing status of cell can be evaluated by the value of aspartokinase (Ask), ε-PL synthetase (Pls) and homoserine dehydrogenase (HSD).
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Hiraki J, Ichikawa T, Ninomiya S, Seki H, Uohama K, Seki H, Kimura S, Yanagimoto Y, Barnett JW (2003) Use of ADME studies to confirm the safety of epsilon-polylysine as a preservative in food. Regul Toxicol Pharmacol 37(2):328–340
Shih IL, Shen MH, Van YT (2006) Microbial synthesis of poly (epsilon-lysine) and its various applications. Biores Technol 97(9):1148–1159
Shima S, Oshima S, Sakai H (1983) Biosynthesis of ɛ-poly-L-lysine by washed mycelium of Streptomyces albulus No. 346. Nippon Nogei Kagaku Kaishi 57:221–226
Hamano Y, Nicchu I, Shimizu T et al (2007) ε-poly-L-lysine producer, Streptomyces albulus, has feedback-inhibition resistant aspartokinase. Appl Microbiol Biotechnol 76(4):873–882
Yamanaka K, Maruyama C, Takagi H et al (2008) ε-poly-L-lysine dispersity is controlled by a highly unusual nonribosomal peptide synthetase. Nat Chem Biol 4(12):766–772
Yoshida T, Nagasawa T (2003) e-poly-L-lysine: microbial production, biodegradation and application potential. Appl Microbiol Biotechnol 62(1):21–26
Itzhaki RF (1972) Colorimetric method for estimating poly-lysine and poly-arginine. Anal Biochem 50(2):569–574
Cao W, Tan Z, Yuan G et al (2007) Improvement of the assay method for ε-poly-lysine. Tianjin Univ Sci Technol 22:9–11 (in Chinese)
Yılmaz EI, Çaydasi AK, Özcengiz G (2008) Targeted disruption of homoserine dehydrogenase gene and its eVect on cephamycin C production in Streptomyces clavuligerus. J Ind Microbiol Biotechnol 35:1–7
Li Y, Chen J, Liang DF et al (2000) Effect of nitrogen source and nitrogen concentration on the production of pyruvate by Torulopsisglabrata. J Biotechnol 81:27–34
Gorret N, Maubois JL, Engasser JM et al (2001) Study of the effects of temperature, pH and yeast extract on growth and exopolysaccharides production by Propionibacterium acidi-propionici on milk microfiltrate using a response surface methodology. J Appl Microbiol 90:788–796
Aeschlimann A, von Stockar U (1990) The effect of yeast extract supplementationion the production of lactic acid from whey permeate by Lactobacillus helveticus. Appl Microbiol Biotechnol 32:398–402
Acknowledgements
We are grateful for financial support from the National Natural Science Foundation of China (Project 21276197), the National High-tech R&D Program (863 Program) (2013AA102106), the Tianjin science and technology commissioner project (15JCTPJC59700) and the National Key Technology Support Program (No. 2015BAD16B04).
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Guo, F., Zheng, H., Zhang, X., Cheng, Y., Tan, Z., Jia, S. (2018). Effect of Yeast Extract on Production of ε-poly-L-lysine by Streptomyces diastatochromogenes . In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_24
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DOI: https://doi.org/10.1007/978-981-10-4801-2_24
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