Abstract
Most of studies have focused on the maca (Lepidium Meyenii) hypocotyls, including the extraction, purification, characterization of bioactive components and their structures, but few on maca leaves. In this paper, we used response surface methodology to optimize the extraction conditions of maca leaves polysaccharides (MLPs). The optimal conditions were as follows: ratio of water to raw material (38 mL/g); extraction time, 150 min; extraction temperature, 100 °C and extraction times, 3. Under the condition, the experimental yield was 14.2 ± 0.25% (mL/g) (n = 3), which was close to the predicted value. The primary characterization of the crude MLPs was explored, and we carried on the FT-IR experiment. The FT-IR analysis revealed the general characteristic absorption peaks of the crude MLPs.
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Wu SH, Gong GL, Wang YY, Li F, Jia SY, Qin FX et al (2013) Response surface optimization of enzyme-assisted extraction polysaccharides from Dictyophoraindusiata. Int J Biol Macromol 61:63–68
Qu Y, Li CX, Zhang C, Zeng R, Fu CM (2016) Optimization of infrared-assisted extraction of Bletillastriata polysaccharides based on response surface methodology and their antioxidant activities. Carbohyd Polym 148:345–353
Zha SH, Zhao QS, Chen JJ, Wang LW, Zhang GF, Zhang H, Zhao B (2014) Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii). Carbohyd Polym 111:584–587
Esparza E, Hadzich A, Kofer W, Mithöfer A, Cosio Eric G (2015) Bioactive maca (Lepidium meyenii) alkamides are a result of traditional Andean postharvest drying practices. Phytochem 116:138–148
Chen JJ, Zhao QS, Wang LW, Zha SH, Zhang LJ, Zhao B (2015) Physicochemical and functional properties of dietary fiber from maca (Lepidium meyenii Walp.) liquor residue. Carbohyd Polym 132:509–512
Uchiyama F, Jikyo T, Takeda R, Ogata M (2014) Lepidium meyenii (Maca) enhances the serum levels of luteinising hormone infemale rats. J Ethnopharmacol 151:897–902
Bai N, He K, Roller M, Lai CS, Bai L, Pan MH (2015) Flavonolignans and other constituents from Lepidium meyenii with activities in anti-inflammation and human cancer cell lines. J Agric Food Chem 63:2458–2463
Liu H, Jin W, Fu C, Dai P, Yu Y, Huo Q, Yu L (2015) Discovering anti-osteoporosis constituents of maca (Lepidium meyenii) by combined virtual screening and activity verification. Food Res 77:215–220
Stojanovska L, Law C, Lai B, Chung T, Nelson K, Day S et al (2015) Maca reduces blood pressure and depression, in a pilot study in postmenopausal women. Climacteric 18:69–78
Sandoval M, Okuhama NN, Angeles FM, Melchor VV, Condezo LA, Lao J et al (2002) Antioxidant activity of the cruciferous vegetable Maca (Lepidium meyenii). Food Chem 79:207–213
Li E, Nie SP, Yang C, Qiu ZH, Xie MY (2011) Extraction optimization, characterization and bioactivity of the crude polysaccharides from Herba Moslae. Carbohyd Polym 83:1201–1206
Wang QH, Shu ZP, Xu BQ, Xing N, Jiao WJ, Yang BY, Kuang HX (2014) Structural characterization and antioxidant activities of polysaccharides from Citrus aurantium L. Int J Biol Macromol 67:112–123
Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Vilkas E, Radjabi NF (1986) The glucomannan system from Aloe vahombe (liliaceae). III. Comparative studies on the glucomannan components isolated from the leaves. Biochimie 68(9):1123–1127
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Li C, Huang Q, Fu X, Yue XJ, Liu RH, You LJ (2015) Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn. Int J Biol Macromol 75:298–305
Acknowledgements
This research was financially supported by the Research Project of People’s Liberation Army (No. AX110C002, BX115C007 and HX-04-13-022).
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Kang, C. et al. (2018). Response Surface Methodology Optimization Extraction of Polysaccharides from Maca (Lepidium meyenii) Leaves and Primary Characterization. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_22
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DOI: https://doi.org/10.1007/978-981-10-4801-2_22
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