Abstract
The steroid 11α-hydroxylase encoded by CYP68J5 in Aspergillus ochraceus TCCC41060 is involved in the 11α-hydroxylation of 16,17α-epoxyprogesterone. To increase the steroid 11α-hydroxylation efficiency of the production strain Aspergillus ochraceus TCCC41060, recombinant strains of A. ochraceus over-expressing CYP68J5 were constructed. Using 16,17α-epoxyprogesterone as substrate (20 g/l) under optimized conditions, recombinant strain CYP68J5-TrpC-2 exhibited conversion rates of 92% compared with 80% for the control strain TCCC41060. Moreover, the time required for the recombinant strain CYP68J5-TrpC-2 to achieve the highest conversion rates (92%) was reduced by 12 h compared with that of the control strain to achieve 80% conversion. It is concluded that recombinant A. ochraceus strains over-expressing the CYP68J5 gene significantly increased conversion rates and dramatically shortened the transformation time, thus improving process efficiency of 11α-hydroxylation of 16,17α-epoxyprogesterone.
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Acknowledgements
This work was supported by National High-tech Research and Development Program of China (863 Program) (no. 2011AA02A211) and Natural Science Foundation of China (no. 21206127).
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Yang, X. et al. (2018). Engineering of Industrial Aspergillus ochraceus Strains for Improved Steroid 11α-Hydroxylation Efficiency via Overexpression of the 11α-Hydroxylase Gene CYP68J5 . In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_21
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DOI: https://doi.org/10.1007/978-981-10-4801-2_21
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