Abstract
This study evaluated extracellular polymer production by Acinetobacter haemolyticus with lychee juice and pulp in cultivation medium. Our earlier studies have established the biotechnological importance (phosphate binding) of polymer produced by this strain. Waste/spoilt lychee juice and homogenised pulp were incorporated in culture medium at concentrations of 5%, 10%, 15%, and 20%, respectively, and extracellular biopolymer was produced, evaluated and compared with biopolymer produced under normal cultivation media for phosphate-binding function, characteristics and toxicological properties. Concentrations of 15% lychee extracts/juice led to comparable biopolymer yield; no significant (p < 0.05) changes were observed in either functionality, characteristics, or yield. Moreover, the biopolymer did not elicit either haemolytic activity or toxicity in RAW cell lines. Haematological, histopathological and general examinations indicated no adverse effects in Swiss albino mice fed with the biopolymer (120 mg kg−1 body weight−1 day1) during a period of 30 days. These results suggested that spoilt lychee can be utilised for production of the biopolymer following the industrial process parameters. Optimisation of scale-up of conditions is, however, required for commercial applicability of utilising the agro-waste to generate this value-added product.
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The work was supported by a grant from the University Grants Commission (UGC), Government of India. We are thankful to Venus Remedies, Baddi, for carrying out the animal studies.
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Ghosh, M. (2017). Lychee Juice and Pulp Extracts as Potential Components for Production of Extracellular Phosphate-Binding Biopolymer from Acinetobacter haemolyticus . In: Kumar, M., Kumar, V., Bhalla-Sarin, N., Varma, A. (eds) Lychee Disease Management. Springer, Singapore. https://doi.org/10.1007/978-981-10-4247-8_11
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DOI: https://doi.org/10.1007/978-981-10-4247-8_11
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