Conventional Versus Modern Approaches in Litchi Tissue Culture

  • Aparna PareekEmail author


The litchi (Litchi chinensis Sonn) is placed among the topmost subtropical evergreen fruit crops, has its origination from China, and then routed to India with ancient and holistic approaches. In India, litchi holds the ranking of seventh in area and ninth in production among fruit crops, but in value forms, it ranks sixth. With the advent of farming and scientific amalgamations litchi production and productivity is constantly being increased in the country. Due to natural variants among litchi genotypes, it offers significant roles in socioeconomics (i.e., nutrition, taste, medicine, etc.). Litchi cultivation is still based on conventional approaches, viz., grafting, air layering, etc., which have wearing and tearing approaches. In current scenario litchi biotechnology is still in scarcity which needs to be enhanced with modern approaches. Here, I propose the potential ways (micropropagation, germplasm culture, anther culture, etc.) to propagate litchi trees with tissue culture approach.


Litchi Fruit crop Genotype Nutrition Micropropagation Anther culture 


  1. Das DK, Rahman A (2012) Expression of a rice chitinase gene enhances antifungal response in transgenic litchi (cv. Bedana). Plant Cell Tissue Organ Cult 109:315–325CrossRefGoogle Scholar
  2. Das DK, Shiva Prakash N, Bhalla-Sarin NB (1999) Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.). Plant Cell Rep 18:691–695CrossRefGoogle Scholar
  3. Deng CJ (2005). Studies on high frequency somatic embryogenesis and regeneration culture in litchi. Master’s thesis of Hunan Agricultural University, Changsha, Hunan, ChinaGoogle Scholar
  4. Fu L, Tang D (1983) Induction pollen plants of litchi tree (Litchi chinensis Sonn.). Acta Genet Sin 10(5):369–374Google Scholar
  5. Guo SY, Peng HX, He XH, Ding F, Li HL, Qin XQ, Xu N (2014) Callus induction from different explants of litchi. Southwest China J Agric Sci 27(2):748–753Google Scholar
  6. Huang ZZ, You KZ (1990) Primary study on the litchi shoots induction. Guangdong Agric Sci 3:27–29Google Scholar
  7. Hwang SC, Ko WH (1987) Somaclonal variation of banana and screening for resistance of Fusarium wilt. In: Persley GJ, De Langhe EA (eds) Banana and plantain breeding strategies. ACIAR proceedings no. 21. ACIAR, Canberra, pp 157–160Google Scholar
  8. Kuang ZS, Zhou LN, Ma XJ, Chen JQ, Cai J (1997) Plant regeneration via somatic embryogenesis in litchi. Guangdong Agric Sci 1:15–17Google Scholar
  9. Litz RE (1988) Somatic embryogenesis from cultured leaf explants of the tropical tree Euphoria longan stend. J Plant Physiol 132:190–193CrossRefGoogle Scholar
  10. Litz RE, Raharjo S (2005) Dimocarpus longan and Litchi chinensis. In: Litz RE (ed) Biotechnology of fruit and nut crops. CABI, Wallingford, pp 628–636CrossRefGoogle Scholar
  11. Menzel CM (1983) The control of floral initiation of lychee: a review. Sci Hortic 21:201–215CrossRefGoogle Scholar
  12. Menzel CM, Waite GK (2005) Litchi and longan: botany, production and uses. CABI, Wallingford, p 305CrossRefGoogle Scholar
  13. Sarin NB (2009) Litchi breeding for genetic improvement. Breeding plantation tree crpos. Tropical speciesGoogle Scholar
  14. Puchooa (2004) In vitro regeneration of lychee (Litchi chinensis Sonn.). Afr J Biotechnol 3(11):576–584Google Scholar
  15. Singh HP (1998) Genetic diversity, breeding and utilisation of the Genepool of Litchi. Tropical Fruits in Asia (Arora and Rao Eds) IPGRI, pp 171–184Google Scholar
  16. Singh HP, Yadav IS (1988) Physico-chemical changes during fruit development in litchi cultivar. India J Hortic 45:212–218Google Scholar
  17. Singh LB, Singh OP (1954) The litchi. Superintendent Printing Press, Allahabad, 110pGoogle Scholar
  18. Wang G, Li HL, Wang JB (2013) Primary study on the callus induction from anther of two litchi (Litchi chinensis Sonn.) cultivars. Chin J Trop Crops 34(4):669–674Google Scholar
  19. Xie YM, Yi GJ, Zhang QM, Zeng JW (2006) Somatic embryogenesis and plantlet regeneration from anther of Feizixiao litchi. Chin J Trop Crops 27(1):68–72Google Scholar
  20. Yu YB (1991) Study on some factors in tissue culture of lychee (Litchi chinensis). Fujian Agric Sci Technol 5:17–18Google Scholar
  21. Yu CH, Chen ZG, Lu LX (1996) Regeneration of plantlets from protoplasts culture of litchi. J Fujian Agric Univ 25(3):386Google Scholar
  22. Yu CH, Chen ZG, Lu LX, Lin JW (2000) Somatic embryogenesis and plantlets from “Xiafanzhi” litchi protoplasts isolated from embryogenic suspensions. Plant Cell Tissue Organ Cult 61:51–58CrossRefGoogle Scholar
  23. Zhou LN, Kuang ZS, Ma XJ, Cheu JQ, Cao J (1996) The study of factors affecting somatic embryogenesis in young embryo culture of Litchi chinensis. J Agric Biotechnol 4(2):161–165Google Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

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