Regeneration from Nodal Explants of Field-Grown Litchi (Litchi chinensis Sonn.) Fruit Trees

  • Vivek Kumar
  • Priyanku Teotia
  • Ram Prasad
  • Ajit Varma
  • Neera Sarin
  • Manoj KumarEmail author


Mass propagation of Litchi chinensis (Sonn.) via seeds is understood as detrimental because of the highly heterozygous nature of the plant due to cross-pollination. The conservative methods of vegetative propagation utilized for litchi are air layering or marcottage, grafting, and budding which are slow and incompetent as evinced by several reports. In the recent past numerous efforts for clonal propagation of litchi were made with marginal success. Earlier our group have reported multiple shoot induction and plant regeneration in litchi from the nodal cuttings and cotyledonary nodes and by in planta treatment of the axillary bud regions. In this, we highlight a research protocol which has a comprehensive discussion. It addresses the technical inputs for reproducible and efficient method of in vitro regeneration of elite litchi trees appropriate for clonal propagation. The protocol that has been referred has been proven advantageous to the horticulturists and the industry for recalcitrant trees, those that can be developed as true to the parental type.


Litchi Heterozygous Grafting Clonal propagation True to the parental type 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  • Vivek Kumar
    • 1
  • Priyanku Teotia
    • 2
  • Ram Prasad
    • 1
  • Ajit Varma
    • 1
  • Neera Sarin
    • 3
  • Manoj Kumar
    • 1
    Email author
  1. 1.Amity Institute of Minorbial TechnologyAmity UniversityNoidaIndia
  2. 2.Laboratory of Microbiology, Department of BotanyCCS UniversityMeerutIndia
  3. 3.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia

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