Abstract
Lignins are phenolic polymers of the plant cell wall and functionally associated with mechanical support, sap conduction, defense mechanisms, strengthening to plant tissue and imperviousness to biodegradation. The biosynthesis of lignin and polyphenol derivatives in living trees, especially in fast growing woody species is known to improve the quality and durability of timber. The biochemical characterization of wood lignin of two elite clones of Hevea brasiliensis (rubberwood), viz. RRII 105, RRII 414 and one wild germplasm accession of Hevea viz. AC 4830 was carried out through Klason lignin analysis, FTIR spectroscopy, Thioacidolysis and GC-MS analysis. FTIR spectroscopy revealed that most of the peaks in the cell wall Residue (CWR) represent major cell wall polysaccharides such as cellulose, hemicelluloses and pectins. When the Klason lignin was subjected to FTIR analysis, the absorbance in the polysaccharide region from 1200 to 900 cm−1 was strongly diminished compared to that of CWR. The peaks at 1627, 1541, 1505, 1461, 1322, 1285 and 1010 cm−1 corresponding to Syringyl (S) and Guaiacyl (G) lignin monomers became more prominent. Thioacidolysis/GC-MS analysis revealed significant variation in the S/G ratio between different clones of Hevea brasiliensis. Corresponding to the variation in total lignin content in three clones, significant variation in S/G ratio was also noticed. RRII 105 and AC 4830 were characterized by high amount of S-lignin in its monomeric composition.
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References
Akerholm M, Salmen L (2001) Interactions between wood polymers studied by dynamic FT-IR spectroscopy. Polymer 42:963–969
Boudet AM (2000) Lignin and lignification: Selected issues. Plant Physiol Biochem 38(1&2):81–96
Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Annu Rev Plant Biol 54:519–546
Chen HM, Han JJ, Cui KM, He XQ (2010) Modification of cambial cell architecture during cambium periodicity in Populus tomentosa Carr. Trees 24:533–540
Dence CW (1992) The determination of lignin. In: Lin SY, Dence CW (eds), Methods in lignin chemistry. Springer-Verlag, Berlin, pp 33–41
Douglas CJ (1996) Phenylpropanoid metabolism and lignin biosynthesis: from weeds to trees. Trends Plant Sci 1:171–178
Faix O (1991) Classification of lignin from different botanical origin by FTIR spectroscopy. Holzforschung 45:21–27
Gnanaharan R, George TK, Damodaran K (2002) Rubber wood processing and utilization in India. Ganesh Publications Pvt. Ltd., Bengaluru 125p
Gierlinger N, Jacques D, Schwanninger M, Wimmer R, Paques LE (2004) Heartwood extractives and lignin content of different of different larch species (Larix sp.) and relationships to brownrot decay-resistance. Trees 18:230–236
Grabber JH, Ralph J, Hatfield RD (1998) Severe inhibition of maize wall degradation by synthetic lignin formed with coniferylaldehyde. J Sci Food Agric 78:81–87
Harrington KJ, Higgins HG, Michell AJ (1964) Infrared spectra of Eucalyptus regnans F. Muell. And Pinus radiate D. Don. Holzforschung 18:108–113
Lapierre C (1993) Applications of new methods for the investigation of lignin structure. In: Jung HG, Buxton DR, Hatfield RD, Ralph J (eds) Forage cell wall structure and digestibility. Madison: American Society Agronomy, pp 133–166
Lapierre C, Pollet B, Ronaldo C (1995) New insights into the molecular architecture of hard wood lignins by chemical degradative methods. Res Chem Intermed 21:397–412
Marga F, Gallo A, Hasenstein KH (2003) Cell wall components affect mechanical properties: studies with thistle flowers. Plant Physiol Biochem 41:792–797
Mellerowicz EJ, Baucher M, Sundberg B, Boerjan W (2001) Unraveling cell wall formation in the woody dicot stem. Plant Mol Biol 47:239–274
Naji HR, Sahri MH, Nobuchi T, Bakar ES (2011) The effect of growth rate on wood density and anatomical charecteristics of rubberwood (Hevea brasiliensis Muell. Arg.) in two different clonal trails. Scholars Research Library. 1:71–80
Pramod S (2012) Structural, histochemical and biochemical studies on xylem cell wall in Leucaena leucocephala (Lam.) De Wit. In: Ph.D. Thesis. Sardar Patel University, Gujarat, India
Rana R, Heyser RL, Finkeldey R, Polle A (2009) FTIR spectroscopy, chemical and histochemical characterization of wood and lignin of five tropical timber wood species of the family Dipterocarpaceae. Wood Sci Technol 44(2):225–242
Rencoret J, Guterrez A, Del-Rio JC (2007) Lipid and lignin composition of wood from different eucalypt species. Holzforschung 61:165–174
Reghu CP, George BP, Varghese YA (2007) Screening of Hevea brasiliensis germplasm for wood quality using cinnamyl alcohol dehydrogenase (CAD) activity and lignification pattern. Nat Rubber Res 20(1&2):1–8
Reghu CP, Mercy MA, Lakshmanan Radha (2012) Further evaluation and selection of 1981 IRRDB wild Hevea germplasm collection in India. Nat Rubber Res 25(1):31–38
Roth R, Boudet AM, Lezika RP (1997) Lignification and cinnamyl alcohol dehydrogenase activity in developing stemsof tomato and poplar: a spatial and kinetic study through printing. J Exp Bot 48(307):247–254
Rubber Grower’s Companion (2014) The rubber board. Kottayam, p 100
Saraswathyamma CK, Thomas V, Soman TA, Meenattoor JR, Mydin KK, Joseph A, Saha T, Chandrasekher TR, Meenakumari T, Sankariammal L, Mercykutty VC, Gireesh T, Suryakumar M, Manju MJ, John A, Santhamma PC, Shankar S (2006) RRII 400 Series Clones of Rubber. In: Brasiliensis H (ed) Rubber Research Institute of India. Rubber Board, Kottayam, Kerala, India, pp 1–51
Teoh YP, Don MM, Ujang S (2011) Assesment of the properties, utilization and preservation of rubberwood (Hevea brasiliensis): a case study in Malaysia. J Wood Sci 57:255–266
Tsutsumi Y, Kondo R, Sakai K, Imamura H (1995) The difference of reactivity between syringyl lignin and guaiacyl lignin in alkaline systems. Holzforschung 49:423–428
Xu F, Sun RC, Lu Q, Jones GL (2006) Comparative anatomy and lignin distribution in normal and tension wood of Salix gordejecii. Wood Sci Technol 40:358–370
Xu F, Liu CF, Ren JL, Sun JX, Sun RC, Curling S, Fowler P, Baird MS (2011) Fractional separation and structural characterization of chlorophyll and lignin from perennial ryegrass (L. perenne) and Cocksfoot grass (D. globerata). Separation Sci Technol 42:1809–1829
Zhao Y, Jiang X, Lu J (2008) Wood charecterization, machining and coating properties of rubberwood plantations in China. In: International workshop on rubberwood processing, technology and promoting sustainable development. Haiku, Dec 2008
Acknowledgements
Pramod S. is thankful to University Grants Commission, New Delhi for the financial support under UGC-DSK Post-Doctoral Fellowship scheme.
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Pramod, S., Reghu, C.P., Rao, K.S. (2017). Biochemical Characterization of Wood Lignin of Hevea brasiliensis . In: Pandey, K., Ramakantha, V., Chauhan, S., Arun Kumar, A. (eds) Wood is Good. Springer, Singapore. https://doi.org/10.1007/978-981-10-3115-1_19
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