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Artificial Rearing of Reduviid Predators for Pest Management pp 121–145Cite as

Body Total Protein and Genomic DNA

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Abstract

Predatory reduviids consume partially digested food. Before consuming the host, predators predigested the host with the help of the salivary enzyme. In order to understand interaction pathways within complex food webs, it is necessary to characterize encounter frequencies between each constituent part of the web and describe the consequence of these interactions. The overall mean protein concentration was high in Spodoptera litura followed by Rhynocoris marginatus, Dysdercus cingulatus, and pig liver and Corcyra cephalonica. Six primers were tested to amplify the DNA of the predator. A total of 52 amplicons were obtained in which four primers in five populations of Rhynocoris marginatus were analyzed. The electrophoretic pattern reveals polymorphism occurred among predator populations. DNA polymorphism with 450 bp was common among three diets such as Corcyra cephalonica weekly once with water, oligidic diet and weekly once Corcyra cephalonica, and Spodoptera litura. Among the six primers, KTG-3 and KTG-5 have amplified better than other primers. The level of genetic diversity is far greater in the oligidic diet-reared Rhynocoris marginatus. Rhynocoris marginatus fed with Spodoptera litura and OD-reared predator yielded consistently high absorbance values followed by Corcyra cephalonica fed

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  1. St. Xavier’s College, Palayamkottai, Tirunelveli, Tamil Nadu, India

    K. Sahayaraj

  2. National Institute of Virology, Alappuzha, Kerala, India

    R. Balasubramanian

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  1. K. Sahayaraj
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  2. R. Balasubramanian
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Sahayaraj, K., Balasubramanian, R. (2016). Body Total Protein and Genomic DNA. In: Artificial Rearing of Reduviid Predators for Pest Management . Springer, Singapore. https://doi.org/10.1007/978-981-10-2522-8_6

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