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Gut Enzyme Profile

  • K. Sahayaraj
  • R. Balasubramanian
Chapter
  • 169 Downloads

Abstract

The diverse feeding habits of Rhynocoris marginatus make the Reduviidae ideal for studies of feeding strategies, including digestive enzyme composition. Wide ranges of digestive enzymes were recorded in the alimentary canal of insects, and their level varies in relation to diet. Digestive enzymes play a major role in insect physiology by converting complex food materials into micromolecules necessary to provide energy and metabolites for growth, development, and other vital functions. These enzymes are produced and distributed in various regions of the gut and salivary gland in different proportions and quantities. It is hypothesized macromolecules in the prey dramatically influence reduviid growth and development mediated through qualitative and quantitative regulation of digestive enzymes. The attempt was made to study the quantitative profile of digestive enzymes in relation to different preys and oligidic diet-reared predator. Rhynocoris marginatus foregut and hindgut contain amylase, protease, invertase, and lipase. Amylase, invertase, and lipase activities of both the foregut and hindgut were high, while R. marginatus was fed with Corcyra cephalonica followed by artificial diet and Spodoptera litura. The hindgut enzyme activities were decreased (++), and similar kinds of observations were recorded for artificial diet and Spodoptera litura. The results show that generally prey type and artificial diet do not have any influence on the enzyme qualitative profile of this reduviid. In salivary gland, all the enzyme activities were moderate (++) except lipase (+) when Corcyra cephalonica and Spodoptera litura provided food. The activity was reduced (+) on Corcyra cephalonica and artificial diet for Rhynocoris marginatus. Results clearly show provision of artificial diet has not changed the enzyme levels in the foregut and midgut of this predator.

Keywords

Macromolecules Salivary gland enzyme profile Extra-oral digestion Digestive enzymes 

References

  1. Agusti N, Cohen AC (2000) Lygus hesperus and L.lineolaris (Hemiptera: Miridae), phytophages, zoophages, or omnivores: evidence of feeding adaptations suggested by the salivary and midgut digestive enzymes. J Entomol Sci 35:176–186Google Scholar
  2. Alomar O, Wiedenmann RN (1996) Zoophytophagous Heteroptera: implications for life history and integrated pest management. Thomas Say Publications in Entomology. Entomological Society of America, LanhamGoogle Scholar
  3. Ambrose DP, Maran SPM (2000) Polymorphic diversity in salivary and haemolymph proteins and digestive physiology of assassin bug Rhynocoris marginatus (Fab.) (Heteroptera : Reduviidae). J Appl Entomol 124:315–317. doi: 10.1046/j.1439–0418.2000.00473.x CrossRefGoogle Scholar
  4. Azevedo DO, Zanuncio JC, Zanuncio JS, Martins GF, Marques-Silva S, Sossai MF, Serrao JE (2007) Biochemical and morphological aspects of salivary glands of the predator Brontocoris tabidus (Heteroptera: Pentatomidae). Braz Arch Biol Technol 50(3):469–477CrossRefGoogle Scholar
  5. Baker JE (1991) Purification and partial characterization of amylase allozmes from lesser grain borer, Rhyzopertha dominica. Insect Biochem 21:303–311CrossRefGoogle Scholar
  6. Balasubramanian TP, Lakshmana Perumal Swamy D, Mohan C, Natarajan R (1975) Cellulolytic activity of marine streptomycetes isolated from the alimentary canal of marine borer. 16th annual conference of AMI, held at virbadhra (U.P.)Google Scholar
  7. Balogun RA, Fisher O (1970) Studies on the digestive enzymes of the common african toad, Bufo regulasis bonlenger. Comp Biochem Physiol 33:813–820CrossRefGoogle Scholar
  8. Baptist BA (1941) The morphology and physiology of the salivary glands of Hemiptera-Heteroptera. Q J Microsc Sci 83:91–139Google Scholar
  9. Boyd DW Jr (2003) Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler) (Hemiptera: Miridae) reflect adaptations for predatory habits. Ann Entomol Soc Am 96(5):667–671CrossRefGoogle Scholar
  10. Boyd DW, Jr (2001) Deraeocoris nebulosus (Uhler) (Hemiptera: Miridae): a potential biological control agent. Ph.D. dissertation, Clemson University, Clemson, SCGoogle Scholar
  11. Boyd DW Jr, Cohen AC, Alverson DR (2002) Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler) (Hemiptera: Miridae) a predacious plant bug. Ann Entomol Soc Am 95:395–401CrossRefGoogle Scholar
  12. Broadway RM, Villani MG (1995) Does host range influence susceptibility of herbivorous insects to non‐host plant proteinase inhibitors? Entomol Exp Appl 76(3):303–312CrossRefGoogle Scholar
  13. Brock RM, Forsberg CW, Buchanan-Smith JG (1982) Proteolytic activity of rumen microorganisms and effect of proteinase inhibitors. Appl Environ Microbiol 44:561–569PubMedPubMedCentralGoogle Scholar
  14. Cobben RH (1979) On the original feeding habits of the Hemiptera (Insecta): a reply to Merrill Sweet. Ann Entomol Soc Am 72:711–715CrossRefGoogle Scholar
  15. Cohen AC (1990) Feeding adaptations of some predaceous Hemiptera. Ann Entomol Soc Am 83(6):1215–1223CrossRefGoogle Scholar
  16. Cohen AC (1993) Organization of digestion and preliminary characterization of salivary trypsin-like enzymes in a predaceousheteropteran, Zelus renardii. J Insect Physiol 39:823–829CrossRefGoogle Scholar
  17. Cohen AC (1995) Extra-oral digestion in predaceous terrestrial Arthropoda. Annu Rev Entomol 40:85–103CrossRefGoogle Scholar
  18. Cohen AC (1996) Plant feeding by predatory Heteroptera: evolutionary and adaptational aspects of trophic switching. In: Alomar O, Wiedenmann RN (eds) Zoophytophagous heteroptera: implicationsfor life history and integrated pest management. Thomas Say Publicationsion Entomology. Entomological Society of America, Lanham, pp 1–17Google Scholar
  19. Cohen AC (1998a) Solid-to-liquid feeding: the inside(s) story of extra-oral digestion in predaceous Arthropoda. Am Entomol 44:103–117CrossRefGoogle Scholar
  20. Cohen AC (1998b) Biochemical and morphological dynamicsand predatory feeding habitsin terrestrial heteroptera. In: Ruberson JR, Coll M (eds) Predaceous heteroptera: implications for biological control. Thomas Say Publications in Entomology. Entomological Society of America, Lanham, pp 21–32Google Scholar
  21. Cohen AC (2000) Feeding fitness and quality of domesticated and feral predators: effects of long-term rearing on artificial diet. Biol Control 13:49–54CrossRefGoogle Scholar
  22. Cohen AC, Wheeler AG Jr (1998) Role of saliva in the highly destructive fourlined plant bug (Hemiptera: Miridae: Mirinae). Ann Entomol Soc Am 91:94–100CrossRefGoogle Scholar
  23. Colebatch G, East P, Cooper P (2001) Preliminary characterisation of digestive proteases of the green mirid, Creontiades dilutus (Hemiptera: Miridae). Insect Biochem Mol Biol 31:415–423CrossRefPubMedGoogle Scholar
  24. Coolbear T, Danial R, Morgan (1992) The enzymes from extreme thermophilies, bacteria sources, thermo strains identified as extremely thermophilic bacilli for extra cellular proteolytic activity and general property of proteinases from two of the strains. J Appl Bacterialogy 71:525Google Scholar
  25. Dani MP, Edwards JP, Richards EH (2005) Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca. Comp Biochem Physiol B Biochem Mol Biol 141(3):373–381CrossRefPubMedGoogle Scholar
  26. Edwards JS (1961) The action and composition of the saliva of an assassin bug Platymeris rhadamanthus Gaerst. (Hemiptera: Reduviidae). J Exp Biol 8:61–77Google Scholar
  27. Grenier S (1977) Effets nocifs de la nipagine M sur le parasitoïde Phryxe caudata [Dipt.: Tachinidae]. Entomophaga 22:23–26CrossRefGoogle Scholar
  28. Heil M, Rita buchler R, Boland W (2004) Quantification of invertase activity in ants under field conditions. J Chem Ecol:RC177–RC183Google Scholar
  29. Hori K (1969) Effect of various activators on the salivary amylase of the bug Lygus disponsi. J Physiol 15:2305–2317Google Scholar
  30. Hori K (1972) Comparative study of a property of salivary amylase among various heteropterous insects. Comp Biochem Physiol 42B:501–508Google Scholar
  31. Hori K (1973) Studies on enzymes, especially amylases, in the digestive system of the bug Lygus disponsiand starch digestion in the system. Res Bull Obihiro Univ 8:173–260Google Scholar
  32. Hori K (2000) Possible causes of disease symptoms resulting from the feeding of phytophagousHeteroptera. In: Schaefer CW, Panizzi AR (eds) Heteroptera of economic importance. CRC, Boca Raton, pp 11–35Google Scholar
  33. House HL (1965) Digestion. In: Rockstein M (ed) Physiology of insecta, vol 2. Academic, New York, pp 818–858Google Scholar
  34. House HL (1967) Artificial diets for insects. A compilation of references and abstracts, Information Bulletin No. 5. Research Institute Canada Dept. Agric, OntarioGoogle Scholar
  35. Ishaaya I, Moore I, Joseph B (1971) Protease and amylase activity in the larvae of the Egyprioan cotton worm, Spodoptera littoralis. J Insect Physiol 17:945–953CrossRefGoogle Scholar
  36. Jayaraman J (1985) Laboratory manual in biochemistry. Wiley Eastern Ltd., New Delhi. pp 75–76, 107Google Scholar
  37. Khan MA (1964) Proteolytic activity in the digestive tract of the water scorpion Laccotrephis maculatus Fab. (Nepidae: Hemiptera). Entomol Experimetalis Applic 7:335–338CrossRefGoogle Scholar
  38. Miles PW (1972) The saliva of hemiptera. Adv Insect Physiol 9:183–256CrossRefGoogle Scholar
  39. Naunoff DG (2001) β‐Fructosidase superfamily: homology with some α‐L‐arabinases and β‐D‐xylosidases. Proteins: Struct Func Bioinf 42(1):66–76CrossRefGoogle Scholar
  40. Nigam CS, Omkar (2003) Experimental animal physiology and biochemistry. New Age International (p) Limited, Publishers, New DelhiGoogle Scholar
  41. Rastogi SC (1962) On the salivary enzymes of some phytophagous and predaceous heteropterans. Sci Cult 28:479–480Google Scholar
  42. Ravikumar T, Albert S, Sanjayan PK (2002) Efficiency of digestion of oligosaccharides in the gut and salivary gland of some seed feeding lygaeids (Heteroptera : Lygaeidae). Entomon 27(1):43–50Google Scholar
  43. Rees AR, Offord RE (1969) Studies on the protease and other enzymes from the venom of Lethocerus cordofanus. Nature 221:665–667CrossRefGoogle Scholar
  44. Sahayaraj K (2004) Reduviids in biological control. In: Sahayaraj K (ed) Indian insect predators in biological control. Dayas Publication, India, pp 134–166. ISBN 8170353408Google Scholar
  45. Sahayaraj K (2007) Isolation, identification and characterization of gut flora of three reduviid predators. Asian J Microbiol Biotechnol Environ Sci 9(4):1073–1075Google Scholar
  46. Sahayaraj K (2014) Reduviids and their merits in biological control. In Basic and applied aspects of biopesticides. Springer, India, p 195–214Google Scholar
  47. Sahayaraj K, Balasubramanian R (2008) Biological control potential evaluation of artificial and factitious diets reared Rhynocoris marginatus (Fab.) on three pest. Arch Phytopathol Plant Protect 42(3):238–247CrossRefGoogle Scholar
  48. Sahayaraj K, Venkatesh P, Balasubramanian R (2007a) Feeding behaviour and biology of a reduviid predator Rhynocoris marginatus (Fabricius) (Heteroptera: Reduviidae) on Oligidic Diet. Hexapoda 14(1):24–30Google Scholar
  49. Sahayaraj K, Kumara Sankaralinkam S, Balasubramanian R (2007b) Prey influence on the salivary gland and gut enzymes qualitative profile of Rhynocoris marginatus (Fab.) and Catamiarus brevipennis (Serville) (Heteroptera: Reduviidae). J Insect Sci 4(4):331–336Google Scholar
  50. Sahayaraj K, Vinoth Kanna A, Muthukumar S (2010) Gross morphology of feeding canal, salivary apparatus and digestive enzymes of salivary gland of Catamirus brevipennis (Servile) (Hemiptera: Reduvidae). J Entomol Res Soc 12(2):37–50Google Scholar
  51. Sahayaraj K, River D, Muthukumar S (2013) Biochemical and electrophoretic analyses of saliva from the predatory reduviid Rhynocoris marginatus (Fab.). Acta Biochim Pol 60(1):91–97Google Scholar
  52. Schaefer CW (1997) The origin of secondary zoophagy from herbivory in Heteroptera (Hemiptera). In: Raman A (ed) Ecology and evolution of plantfeeding insects in natural and man-made environments. International ScientiÞc Publications, New Delhi, pp 229–239Google Scholar
  53. Simpson SJ, Raubenheimer D (1993) A multi-level analysis of feeding behaviour: the geometry of nutritional decisions. Philos Trans R Soc Lond B Biol Sci 342(1302):381–402.bbGoogle Scholar
  54. Sunderland KD (1988) Quantitative methods for detecting invertebrate predation occurring in the field. Ann Appl Biol 112:201–224CrossRefGoogle Scholar
  55. Sweet MH (1979) On the original feeding habitsof hemiptera: Insecta). Annu Entomol Soc Am 72:575–579CrossRefGoogle Scholar
  56. Takanona T, Hori K (1974) Digestive enzymes in the salivary gland and midgut of the bug Stenotus binotatus. Comp Biochem Physiol 47A:521–528CrossRefGoogle Scholar
  57. Taylor GS, Miles PW (1994) Composition and variability of the saliva of coreids in relation to phytoxicoses 400 and other aspects of the salivary physiology of phytophagous heteroptera. Entomol Exp Appl 73:265–277CrossRefGoogle Scholar
  58. Tonapi TG (1996) Experimental entomology. CBS Publishers of Distributors, New DelhiGoogle Scholar
  59. Wheeler AG Jr (2001) Biology of the plant bugs (Hemiptera: Miridae): pests, predators, opportunists. Cornell University Press, IthacaGoogle Scholar
  60. Wigglesworth VB (1972) The principles of insect physiology, 7th edn. Chapman and Hall, London, p 827Google Scholar
  61. Xie ZN, Nettles WC Jr, Morrison RK, Irie K, Vinson SB (1986) Three methods for the in vitro culture of Trichogramma pretiosum Riley. J Entomol Sci 21:133–138Google Scholar
  62. Yazgan S, House HL (1970) An hymenopterous insect, the parasitoid Itoplectis conquisitor reared axenically on a chemically defined synthetic diet. Can Entomol 102:1304–1306CrossRefGoogle Scholar
  63. Yazlovetsky IG (1992) Development of artificial diets for entomophagous insects by under standing their nutrition and digestion. In: Anderson TE, Leppla NC (eds) Advances in insect rearing for research and pest management. Westview Press, Boulder, pp 41–62Google Scholar
  64. Zeng F, Cohen AC (2000a) Comparisonof amylase and protease activities of a zoophytophagous and two phytozoophagous Heteroptera. Comp Biochem Physiol 126A:101–106CrossRefGoogle Scholar
  65. Zeng F, Cohen AC (2000b) Demonstration of amylase from the zoophytophagous anthocorid Orius insidiosus. Arch Insect Biochem Physiol 44:136–139CrossRefPubMedGoogle Scholar
  66. Zeng F, Cohen AC (2001) Induction of elastase in a zoophytophagous heteropteran, Lygus Hesperus (Hemiptera: Miridae). Ann Entomol Soc Am 94:146–151CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • K. Sahayaraj
    • 1
  • R. Balasubramanian
    • 2
  1. 1.St. Xavier’s College, PalayamkottaiTirunelveliIndia
  2. 2.National Institute of VirologyAlappuzhaIndia

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