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Gut Autochthonous Microbes and Their Enzyme Profile

  • K. Sahayaraj
  • R. Balasubramanian
Chapter
  • 178 Downloads

Abstract

Insects dependent on various diets commonly carry symbiotic microorganisms that provide nutritional supplements for their host. Since antimicrobial agent has been reared in oligidic diet to prevent microbial contamination, it is essential to study the microbiological nature of symbiotic bacteria on artificial diet-reared predators. Studies of associations between artificial diet natural prey feeding and gut microorganisms of insect predators have sharpened appreciation for utilizing the diet in predator rearing gained importance. In this study, it tested that artificial diet has any influence on microbial community composition in Rhynocoris marginatus adults compared with natural prey source. The study suggested that the total heterotrophic bacterial population (THBP) is varied in the diet ingestion by the reduviid Rhynocoris marginatus. Among all the categories, the bacterial population was found to be higher in Spodoptera litura category followed by Corcyra cephalonica, Corcyra cephalonica with water, and oligidic diet with Corcyra cephalonica. THBP was drastically decreased in artificial diet-reared reduviids. It showed that microbe’s population was drastically altered if the food nature changed. Natural food like Spodoptera litura keeps the gut microbes viable and enriches gut natural population. A total of 14 bacterial species were recorded in artificial diet-reared Rhynocoris marginatus, followed by the Corcyra cephalonica (9) and Spodoptera litura (9). Micrococcus sp., Bacillus subtilis, Pseudomonas fluorescens, Streptococcus faecalis, and Aeromonas sp. were also isolated from the predators. Micrococcus varians was a dominant (52.70 %) species diet.

In natural prey-reared reduviid, the number of bacterial cells remained almost constant during the experiment, while the number of bacteria than other artificial diets increased. In contrast, the number of bacterial species within artificial diet-reared insect decreased. In natural prey, Spodoptera litura, nine bacteria were observed with varying numbers of bacterial cells. In T1 categories, ten bacteria species were isolated followed by species in T3 categories (artificial diet). Fourteen bacterial species were isolated from T4 category (artificial diets + Corcyra cephalonica).

Keywords

Insect gut Microbial diversity Total heterotrophic bacterial populations (THBP) Hydrolytic extracellular enzyme 

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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • K. Sahayaraj
    • 1
  • R. Balasubramanian
    • 2
  1. 1.St. Xavier’s College, PalayamkottaiTirunelveliIndia
  2. 2.National Institute of VirologyAlappuzhaIndia

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