Abstract
Arginase I is a metalloenzyme that catalyses arginine into urea and ornithine. It plays an essential role in hepatic metabolism of most ureotelic organisms as a principal component of urea cycle responsible for elimination of nitrogenous waste (ammonia) in the form of urea. Arginase I is predominantly expressed in the cytoplasm of liver but is less expressed in other tissues. Changes in the expression of arginase I is regulated by various factors including dietary and various hormones. We were interested to know the regulation of arginase I by dexamethasone in the liver of mice at two different ages: young (1-month) and old (18-month). Our results indicate that dexamethasone significantly upregulates the activity of arginase I in both young and old mice, albeit to a varying degree with young mice showing a higher magnitude of upregulation as compared to the old mice. The enzyme protein level ascertained by Western blotting also matched that of the activity. Arginase I mRNA level paralleled that of protein level on such treatments. These results show that dexamethasone mediated induction of liver arginase I expression declines with age of mice.
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Acknowledgments
Authors express gratitude to the Department of Biochemistry, North-Eastern Hill University, Shillong for providing research facilities and infrastructures under UGC-UPE, DRS and DST-FIST, New Delhi. TM is also grateful to CSIR, New Delhi for the support financially under JRF and SRF fellowship (F.No: 09/347(0194)/2010-EMR-I).
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Majaw, T., Sharma, R. (2017). Arginase I Regulation by Dexamethasone in the Liver of Aging Mouse. In: Rath, P., Sharma, R., Prasad, S. (eds) Topics in Biomedical Gerontology. Springer, Singapore. https://doi.org/10.1007/978-981-10-2155-8_6
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DOI: https://doi.org/10.1007/978-981-10-2155-8_6
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