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Proceedings of the First International Conference on Intelligent Computing and Communication pp 25–35Cite as

Computational Molecular Analysis of Human Rhodopsin, Transducin and Arrestin Interactions: An Insight into Signal Transduction for Ophthalmology

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Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 458))

Abstract

Retinal G-protein receptor; rhodopsin upon light-activation, gets phosphorylated, experiences conformational shift and interacts with G-protein; transducin. To completely obstruct the signal transduction visual protein; arrestin binds consecutively to disrupt the cationic channels of plasma membrane. Experimented binding assays documents the protein interactions but hitherto computational investigation was undone. This probe aims at the computational study of conformational alterations in rhodopsin upon sequential interactions, accompanied by variations in its surface electrostatic potential and net solvent accessible area. 3D structures of human transducin, arrestin and rhodopsin were analyzed. Residual participation from the optimized and simulated trio-complex (rhodopsin-transducin-arrestin) disclosed that predominantly positively charged amino-acid residues; Arg474, Arg412, Arg229, Arg13, Lys15 and Lys408 from rhodopsin participated with transducin and arrestin forming 9 ionic interactions. Rhodopsin was perceived to interact in a gradual firmer pattern with its partner proteins. This study presents a novel viewpoint into the computational disclosure for participation of concerned visual proteins.

Tanushree Mukherjee and Arundhati Banerjee are equal contributor.

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Abbreviations

MD:

Molecular Dynamics

P.I.C.:

Protein Interaction Calculator

S1:

Rhodopsin before any Interaction

S2:

Rhosopsin after Interaction with Transducin

S3:

Rhodopsin after Interaction with Arrestin in presence of Transducin

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Acknowledgments

Authors are grateful for continuous encouragement provided by Dr. Angshuman Bagchi, Assistant Professor, Department of Biochemistry and Biophysics, University of Kalyani, India. Authors also deeply acknowledge Department of Biotechnology from National Institute of Technology Durgapur, India and Bengal College of Engineering and Technology, India for their cooperation.

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Authors and Affiliations

  1. Department of Biotechnology, Bengal College of Engineering & Technology, Durgapur, India

    Tanushree Mukherjee &  Sujay Ray

  2. Department of Biotechnology, National Institute of Technology, Durgapur, India

    Arundhati Banerjee

  3. Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, Nadia, India

    Sujay Ray

Authors
  1. Tanushree Mukherjee
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  2. Arundhati Banerjee
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  3. Sujay Ray
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Corresponding author

Correspondence to Sujay Ray .

Editor information

Editors and Affiliations

  1. Department of CSE, University of Kalyani, Kalyani, West Bengal, India

    Jyotsna Kumar Mandal

  2. Department of Computer Science & Engineering, Anil Neerukonda Institute of Technology and Sciences, Vishakapatnam, Andhra Pradesh, India

    Suresh Chandra Satapathy

  3. J K Mandal, Dept of CSE, University of Kalyani, Kalyani, West Bengal, India

    Manas Kumar Sanyal

  4. Department of ECE, Sri Ramswaroop Memorial College of Engineering and Management Lucknow, Lucknow, Uttar Pradesh, India

    Vikrant Bhateja

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Tanushree Mukherjee, Arundhati Banerjee, Sujay Ray (2017). Computational Molecular Analysis of Human Rhodopsin, Transducin and Arrestin Interactions: An Insight into Signal Transduction for Ophthalmology. In: Mandal, J., Satapathy, S., Sanyal, M., Bhateja, V. (eds) Proceedings of the First International Conference on Intelligent Computing and Communication. Advances in Intelligent Systems and Computing, vol 458. Springer, Singapore. https://doi.org/10.1007/978-981-10-2035-3_4

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  • DOI: https://doi.org/10.1007/978-981-10-2035-3_4

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-2034-6

  • Online ISBN: 978-981-10-2035-3

  • eBook Packages: EngineeringEngineering (R0)

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