Abstract
The main objective of the study is to enhance the cellulase production using Plackett Burman Design (PBD) and Central Composite Design (CCD) by Aspergillus nidulan in Submerged fermentation (SmF). The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Yeast extract, MnSO4·H2O, avicel and beef extract were selected based on their positive influence on cellulase production. The selected components were optimized using Response Surface Methodology (RSM). At the optimum condition, a maximum cellulase production of 11.6 IU/mL was obtained.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Akpinar, M., OzturkUrek, R.: Production of ligninolytic enzymes by solid state fermentation using Pleurotus eryngii. Prep. Biochem. Biotechnol. 42, 582–597 (2012)
Dashtban, M., Schraft, H., Qin, W.: Fungal bioconversion of lignocellulosic residues; opportunities and perspectives. Int. J. Biol. Sci. 5(6), 578–595 (2009)
Ghose, T.K.: Measurement of cellulase activities. Pure Appl. Chem. 59(2), 257–268 (1987)
Ping, L., Brosse, N., Sannigrahi, P., Ragauskas, A.: Evaluation of grape stalks as a bioresource. Ind. Crops Prod. 33, 200–204 (2011)
Riswanali, S.B., Saravanan, P., Muthuvelayudham, R., Viruthagiri, T.: Optimization of nutrient medium for cellulase and hemicellulase productions from rice straw: a statistical approach. Int. J. Chem. Anal. Sci. 3(4), 1364–1370 (2012)
Saravanan, P., Muthuvelayudham, R., Viruthagiri, T.: Application of statistical design for the production of cellulase by Trichoderma reesei using mango peel. Enzyme Res. Article ID 157643, 7 pages (2012)
Singhania, R.R., Patel, A.K., Zoclo, C.R., Pandey, A.: Recent advances in solid-state fermentation. Biochem. Eng. J. 44, 13–18 (2009)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media Singapore
About this chapter
Cite this chapter
Saravanan, P., Muthuvelayudham, R. (2016). Enhancing the Bioproduction of Cellulase by Aspergillus nidulan via Medium Optimization. In: B. D., P., Gummadi, S., Vadlani, P. (eds) Biotechnology and Biochemical Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-1920-3_7
Download citation
DOI: https://doi.org/10.1007/978-981-10-1920-3_7
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-1919-7
Online ISBN: 978-981-10-1920-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)