Abstract
In this present study, pectin extraction was performed and optimized using sunflower heads (Helianthus annuus) as a raw material. The effect of microwave power, pH, time, and solid-liquid ratio was investigated using of Box-Behnken response surface experimental design. The experimental results were analyzed by least square regression analysis (ANOVA) method and a second order polynomial model was constructed for response from the experimental data. The model constructed was sufficient to explain the relationship between different variables such as effect of microwave power, pH, time, solid-liquid ratio and response yield (%). The optimized extraction conditions for the highest pectin yield were found to be: microwave power 482.58 W, pH 1.37, time 35.34 s and solid-liquid ratio 36.15 g/100 ml. These results suggest that microwave assisted extraction of sunflower heads could be a best option for extraction of functional pectin at lab scale level.
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References
Agarwal, A.K., Goyal, P., Lakashminarasimhaiah, P.G., Desai, P., Singh, G.K.: Isolation of pectin from emblica officinalis & Linum unitatissimum using microwave assisted extraction technique and compare with conventional extraction method. Int. J. Pharma Res. Rev. 4(2), 16–20 (2015)
El-Nawawi, S.A., Shehata, F.R.: Effect of the extraction temperature on the characteristics of pectin extracted from Egyptian orange peel. Biol. Wastes 24, 307–311 (1988)
Faravash, R.S., Ashtiani, F.Z.: The effect of pH, ethanol volume and acid washing time on the yield of pectin extraction from peach pomace. Int. J. Food Sci. Technol. 42, 1177–1187 (2007)
Fishman, M.L., Chau, H.K., Hoagland, P.D., Hotchkiss, A.T.: Microwave-assisted extraction of lime pectin. Food Hydrocolloids 20(8), 1170–1177 (2006)
Guo, Z.K., Jin, Q.H., Fan, G.Q., Duan, Y.P., Qin, C., Wen, M.J.: Microwaveassisted extraction of effective constituents from a Chinese herbal medicine Radix puerariae. Anal. Chim. Acta 436, 41–47 (2001)
Koh, P.C., Leong, C.M., Mohd Adzahan, N.: Microwave-assisted extraction of pectin from jackfruit rinds using different power levels. Int. Food Res. J. 21(5), 2091–2097 (2014)
Kratchanova, M., Pavlova, E., Panchev, I.: The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohydr. Polym. 56(2), 181–185 (2004)
Kute, A., Mohapatra, D., Babu, B., Sawant, B.P.: Optimization of microwave assisted extraction of pectin from orange peel using response surface methodology. J. Food Res. Technol. 3(2), 62–70 (2015)
Liu, L., Fishman, M.L., Kost, J., Hicks, K.B.: Pectin-based systems for colon-specific drug delivery via oral route. Biomaterials 24(19), 3333–3343 (2003)
Li, J., Zu, Y.-G., Fu, Y.-J., Yang, Y.-C., Li, S.-M., Li, Z.-N., et al.: Optimization of microwave-assisted extraction of triterpene saponins from defatted residue of yellow horn (Xanthoceras sorbifolia Bunge.) kernel and evaluation of its antioxidant activity. Innovative Food Sci. Emerg. Technol. 11(4), 637–643 (2010)
Maran, J.P., Manikandan, S., Thirugnanasambandham, K., VignaNivetha, C., Dinesh, R.: Box-Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydr. Polym. 92, 604–611 (2014)
Morris, G.A., Ralet, M.-C., Bonnin, E., Thibault, J.-F., Harding, S.E.: Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin. Carbohydr. Polym. 82(4), 1161–1167 (2010)
Nwabueze, T.U.: Review article: basic steps in adapting response surface methodology as mathematical modelling for bioprocess optimisation in the food systems. Int. J. Food Sci. Technol. 45(9), 1768–1776 (2010)
Prakash Maran, J., Mekala, V., Manikandan, S.: Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata. Carbohydr. Polym. 92(2), 2018–2026 (2013)
Rahmati, S., Abdullah, A., Momeny, E., Kang, O.L.: Optimization studies on microwave assisted extraction of dragon fruit (Hylocereus polyrhizus) peel pectin using response surface methodology. Int. Food Res. J. 22, 233–239 (2015)
Shanmugaprakash, M., Sivakumar, V.: Development of experimental design approach and ANN-based models for determination of Cr (VI) ions uptake rate from aqueous solution onto the solid biodiesel waste residue. Bioresour. Technol. 148, 550–559 (2013)
Shanmugaprakash, M., Kirthika, J., Ragupathy, J., Nilanee, K., Manickam, A.: Statistical based media optimization and production of naringinase using Aspergillus brasiliensis 1344. Int. J. Biol. Macromol. 64, 443–452 (2014)
Silva, S., Martins, S., Karmali, A., Rosa, E.: Production, purification and characterisation of polysaccharides from Pleurotus ostreatus with antitumour activity. J. Sci. Food Agric. 92(9), 1826–1832 (2012)
Srivastava, P., Malviya, R.: Sources of pectin, extraction and its applications in pharmaceutical industry-an overview. Indian J. Nat. Prod. Resour. 2(1), 10–18 (2011)
Thetsrimuang, C., Khammuang, S., Chiablaem, K., Srisomsap, C., Sarnthima, R.: Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév. Food Chem. 128(3), 634–639 (2011)
Venkatesh Prabhu, M., Karthikeyan, R., Shanmugaprakash, M.: Modeling and optimization by response surface methodology and neural network–genetic algorithm for decolorization of real textile dye effluent using Pleurotus ostreatus: a comparison study. Desalin. Water Treat. 57, 1–15 (2015)
Wang, S.J., Chen, F., Wu, J.H., Wang, Z.F., Liao, X.J., Hu, X.S.: Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology. J. Food Eng. 78(2), 693–700 (2007)
Yoo, S.H., Lee, B.H., Lee, H., Lee, S., Bae, I.Y., Lee, H.G., Jr Hotchkiss, A.T., et al.: Structural characteristics of pumpkin pectin extracted by microwave heating. J. Food Sci. 77(11), C1169–C1173 (2012)
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The authors are thankful to the management of Kumaraguru College of Technology, Coimbatore, India, for the encouragement and providing the research facilities.
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Aarthi, B.K., Aswini, V., Lakshmi Priya, M., Nirosha, M., Shanmugaprakash, M. (2016). Optimization of Microwave Assisted Extraction of Pectin from Helianthus annuus Head Using Response Surface Methodology. In: B. D., P., Gummadi, S., Vadlani, P. (eds) Biotechnology and Biochemical Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-1920-3_4
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DOI: https://doi.org/10.1007/978-981-10-1920-3_4
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