Skip to main content
SpringerLink
Log in

Statistical Optimization of Lactic Acid Extraction from Fermentation Broth Using Emulsion Liquid Membrane

  • Chapter
  • First Online:
Biotechnology and Biochemical Engineering

Abstract

The performance of emulsion liquid membrane (ELM) for lactic acid extraction from fermentation broth was investigated. Fermentation broth containing lactic acid (LA) was obtained from the fermentation of sugarcane molasses using corn steep liquor (CSL) as nitrogen source by immobilized Lactobacillus casei MTCC 1423 cells after 60 h of incubation time. Lactic acid extraction using ELM from fermentation broth was investigated. Extraction efficiency, η ext (82 %) after 13.5 min of the batch extraction time was obtained and also observed that the stability of the emulsion was affected in the presence fermentation broth constituents. Keeping in view the stability aspect of emulsion liquid membrane for in situ lactic acid extraction during fermentation, process optimization of the process variables for maximizing the lactic acid extraction from fermentation broth using response surface methodology was carried out. Central composite rotatable design (CCRD) for three variables viz., span 80 concentration, c s ; TOA concentration, ψ; batch extraction time, τ at five levels for lactic acid extraction from fermentation broth has been employed. A numerical optimization technique was applied to find the optimum conditions for maximizing the extraction of lactic acid from fermentation broth and the optimum conditions obtained for maximum lactic acid extraction efficiency, η ext (82.67 %) were: span 80 concentration, c s : 5 % (v/v); TOA concentration, ψ: 9.5 % (v/v); batch extraction time τ: 28 min. The results suggest that the ELM has significant potential for in situ lactic acid extraction.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Ani, I., Wahidin, S.: Effect of sodium alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii. Process Biochem. 41, 1117–1123 (2006)

    Article  Google Scholar 

  • Dan, C.V., Adriana, P., Fancisc, V.D., Carmen, S.: HPLC characterization of lactic acid formation and ftir fingerprint of probiotic bacteria during fermentation processes. Not. Bot. Horti. Agrobo. 38(1), 109–113 (2010)

    Google Scholar 

  • Dey, P., Pal, P.: Direct production of l(+) lactic acid in a continuous and fully membrane-integrated hybrid reactor system under non-neutralizing conditions. J. Membr. Sci. 389, 355–362 (2012)

    Article  CAS  Google Scholar 

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Robers, P.A., Smith, F.: Colorimetric method for determination of sugar and related substances. Anal. Chem. 28, 350–356 (1996)

    Article  Google Scholar 

  • Farshid, G., Habibollah, Y., Abbas, E.S., Ghasem, N.: Cane molasses fermentation for continuous ethanol production in an immobilized cells reactor by Saccharomyces cerevisiae. Renew. Ener. 36, 503–509 (2011)

    Article  Google Scholar 

  • Ginjupalli, K., Armugam, K., Shavi, V.G., Averineni, R.K., Mahalingam, B., Udupa, N.: Development of RP-HPLC method for simultaneous estimation of lactic acid and glycolic acid. Der Pharma Chemica. 5(4), 335–340 (2013)

    Google Scholar 

  • Goyal, R.K., Jayakumar, N.S., Hashim, M.A.: Chromium removal by emulsion liquid membrane using [BMIM]+[NTf2]—as stabilizer and TOMAC as extractant. Desalination 278, 50–56 (2011)

    Article  CAS  Google Scholar 

  • Hongpu, J., Wei, P., Juntong, Z., Chunjian, X.: Extraction performance of bisphenol A from aqueous solutions by emulsion liquid membrane using response surface methodology. Desalination 313, 36–43 (2013)

    Article  Google Scholar 

  • Imdad, K., Huang, S., Bo, L., Bin, W., Ra, Aamir, Chun, L.: Efficient biosynthesis of glycyrrhetic acid 3-O-mono-β-D-glucuronide(GAMG) in water-miscible ionic liquid by immobilized whole cells of Penicillium purpurogenum Li-3 in alginate gel. Chem. Eng. Sci. 106, 136–143 (2014)

    Article  Google Scholar 

  • Jia, O., Rui, M., Zhaojuan, Z.C.C., Min, Z., Ting, J.: Open fermentative production of L-lactic acid by Bacillus sp. strain NL01 using lignocellulosic hydrolyzates as low-cost raw material. Bioresour. Technol. 135, 475–480 (2013)

    Article  Google Scholar 

  • Joglekar, H.G., Imran, R., Suresh, B., Kulkarni, B.D., Ajit, J.: Review-comparative assessment of downstream processing options for lactic acid. Sep. Purif. Technol. 52, 1–17 (2006)

    Article  CAS  Google Scholar 

  • Julio, B., Leo, D.P., Geran, A.: Gibberellic acid extraction from aqueous solutions and fermentation broths by using emulsion liquid membranes. J. Membr. Sci. 348, 91–98 (2010)

    Article  Google Scholar 

  • Ke, X., Ping, X.: Efficient production of L-lactic acid using co-feeding strategy based on cane molasses/glucose carbon sources. Bioresour. Technol. 153, 23–29 (2014)

    Article  Google Scholar 

  • Klinkenberg, G., Lystad, K.Q., Levine, D.W., Dyrset, N.: Cell release from alginate immobilized Lactococcus lactis ssp. lactis in chitosan and alginate coated beads. J. Dairy Sci. 84, 1118–1127 (2010)

    Article  Google Scholar 

  • Lucas, D., Phylipe, A. C., Julio, L. d. S. J., Leonardo, H. d. O., Martin, A.: Liquid equilibrium data for ternary systems of water + lactic acid + C4-C7 alcohols at 298.2 K and atmospheric pressure, Fliuid Phase Equilibr. 354, 12–18 (2013)

    Google Scholar 

  • Min-Tian, G., Takashi, S., Nobuhiro, I., Haruo, T.: Fermentative lactic acid production with a metabolically engineered Yeast immobilized in photo-crosslinkable resins. Biochem. Eng. J. 47, 66–70 (2009)

    Article  Google Scholar 

  • Mohamed, A.Abdel-R, Yukihiro, T., Kenji, S.: Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria, overview and limits. J. Biotechnol. 156, 286–301 (2011)

    Article  Google Scholar 

  • Mokhtari, B., Pourabdollah, K.: Inclusion desalination of alkali metal cations by emulsion liquid membranes and nano-baskets of p-tert-calix105arene bearing di-[N-(X)sulfonylcarboxamide] and di-(1-propoxy) in para-cone conformation. Desalination, 292, 1–8 (2012)

    Google Scholar 

  • Mrinal, N.K., Angelika-Ioanna, G., Jean Bernard, M., Panagiotis, K., Argyro, B., Athanasios, A.K., Maria, K.: Lactic acid fermentation by cells immobilised on various porous cellulosic materials and their alginate/poly-lactic acid composites. Bioresour. Technol. 165, 332–335 (2014)

    Article  Google Scholar 

  • Niju, N., Pradip, K.R., Aradhana, S.: l(+) lactic acid fermentation and its product polymerization. Electron. J. Biotechn. 7(2), 167–179 (2004)

    Google Scholar 

  • Pattana, L., Arthit, T., Vichean, L., Lakkana.: L. Acid hydrolysis of sugarcane bagasse for lactic acid production. Bioresour. Technol. 101, 1036–1043 (2010)

    Google Scholar 

  • Prasad, S.K., Shrikant, J.W., Vijaykumar, V.M.: Process intensification in extraction by liquid emulsion membrane (LEM) process, a case study; enrichment of ruthenium from lean aqueous solution. Chem. Eng. Process. 49, 441–448 (2010)

    Article  Google Scholar 

  • Raja, N.R.S., Norasikin, O., Nor, A.S.A.: Emulsion liquid membrane stability in the extraction of ionized nanosilver from wash water. J. Ind. Eng. Chem. 20, 3243–3250 (2014)

    Article  Google Scholar 

  • Se-Kwon, M., Young-Jung, W., Gi-Wook, C.: A novel lactic acid bacterium for the production of high purity l-lactic acid, Lactobacillus paracasei subsp. paracasei CHB2121. J. Biosci. Bioeng. 114, 155–159 (2012)

    Article  Google Scholar 

  • Sushil, K., Dipaloy, D., Babu, B.V.: Estimation of equilibrium parameters using differential evolution in reactive extraction of propionic acid by tri-n-butyl phosphate. Chem. Eng. Process. 50, 614–622 (2011)

    Article  Google Scholar 

  • Tayyba, G., Muhammad, I., Zahid, A., Tahir, A., Zubia, Z., Asma, T., Muhammad, K., Nudrat, E., Sajid, M.: Recent trends in lactic acid biotechnology, a brief review on production to purification. J. Rad. Res. Ap. Ac. 7, 222–229 (2014)

    Google Scholar 

  • Umar, F., Faqir, M.A., Tahir, Z., Sajjad-U-R., Muhammd, A.R., Anwaar, A., Kashif, A.: Optimization of lactic acid production from cheap raw material, sugarcane molasses. Pak. J. Bot. 44(1), 333–338 (2012)

    Google Scholar 

  • Xiaolin, W., Yaoming, W., Xu, Z., Tongwen, X.: In situ combination of fermentation and electrodialysis with bipolar membranes for the production of lactic acid, operational compatibility and uniformity. Bioresour. Technol. 125, 165–171 (2012)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Sant Longowal Institute of Engineering and Technology, Longowal, Sangrur, 148106, Punjab, India

    Avinash Thakur

  2. Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, Sangrur, 148106, Punjab, India

    Parmjit Singh Panesar

  3. Guru Nanak Dev Engineering College, Ludhiana, Punjab, India

    Manohar Singh Saini

Authors
  1. Avinash Thakur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Parmjit Singh Panesar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Manohar Singh Saini
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Avinash Thakur .

Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, National Institute of Tech Karnataka, Mangalore, Karnataka, India

    Prasanna B. D.

  2. Bhupat and Jyoti Mehta School of Bioscie, Indian Institute of Technology Madras, Chennai, Tamil Nadu, India

    Sathyanarayana N Gummadi

  3. Department of Chemical Engineering, Kansas State University, Manhattan, Kansas, USA

    Praveen V. Vadlani

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media Singapore

About this chapter

Cite this chapter

Thakur, A., Panesar, P.S., Saini, M.S. (2016). Statistical Optimization of Lactic Acid Extraction from Fermentation Broth Using Emulsion Liquid Membrane. In: B. D., P., Gummadi, S., Vadlani, P. (eds) Biotechnology and Biochemical Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-1920-3_3

Download citation

Publish with us

Policies and ethics

Search

Navigation