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Application of Tissue Culture for Laburnum anagyroides Medik. Propagation

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Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Abstract

Laburnum anagyroides, a small tree or shrub from the Fabaceae family, is a promising object for its use in decorative landscaping and as a source of pharmaceuticals. The conventional propagation methods are not always successful for L. anagyroides. Herein, we describe an approach involving the application of tissue culture techniques for its micropropagation. This approach is based on activation of the pre-existing meristems from the axillary buds taken from a mature tree or seedling explants and includes the following phases: (1) preparation of primary explants and their cultivation on the Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzylaminopurine (BAP), hot water pretreatment with subsequent cultivation of the seeds on a MS medium used to overcome the physical dormancy of seeds, (2) proliferation of the initiated explants on the full-strength (for seedling explants) or ½ MS medium (for axillary buds) with 2.22 μM BAP, (3) rooting of individual shoots on the ¼ MS medium supplemented with 2.68 μM α-naphthaleneacetic acid, and (4) acclimatization of the plants by spraying with the Emistim® elicitor. It was found that the explanting season also affected the initiation frequency from axillary buds but did not influence the culture initiation from seedling explants. In the tissue culture of both buds and seedlings, BAP not only stimulated higher number of shoots but also ensured the development of normal shoots compared with the thidiazuron-containing medium. The results of our study can be used for the mass propagation of L. anagyroides and for obtaining good-quality seedlings suitable for gardening and for pharmaceutical industry.

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References

  • Ahmad N, Anis M (2007) Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary s hoots proliferation. Agrofor Syst 71:195–200

    Article  Google Scholar 

  • Ahuja MR (1993) Micropropagation of woody plants. Kluwer, Dordrecht

    Book  Google Scholar 

  • Aloufa M, Bezerra S, Jordâo G (2003) In vitro clonal mass propagation of Ximenia Americana L. Fruits 58:175–178

    Article  Google Scholar 

  • Anis M, Varshney A, Siddigue I (2010) In vitro clonal propagation of Balanites aegyptiaca (L.) Del. Agrofor Syst 78:151–158

    Article  Google Scholar 

  • Arrilaga I, Marzo T, Segura J (1991) Micropropagation of juvenile and adult Sorbus domestica L. Plant Cell Tissue Organ Cult 27:341–348

    Article  Google Scholar 

  • Baskin CC, Baskin JM (1998) Seeds; ecology, biogeography and evolution of dormancy and germination. Academic, San Diego

    Google Scholar 

  • Baskin JM, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14:1–16

    Google Scholar 

  • Beck SL, Dunlop RW (2001) Micropropagation of the Acacia species – a review. In Vitro Cell Dev Biol Plant 37:531–538

    Article  Google Scholar 

  • Benson EE (2000) In Vitro plant recalcitrance: an introduction. In Vitro Cell Dev Biol—Plant 36:141–148

    Google Scholar 

  • Conde P, Sousa A, Costa A, Santos C (2008) A protocol for Ulmus minor Mill. micropropagation and acclimatization. Plant Cell Tissue Organ Cult 92:113–119

    Article  Google Scholar 

  • Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1:155–161

    Article  Google Scholar 

  • Giri CC, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18:115–135

    Article  Google Scholar 

  • Gomes F, Canhoto JM (2008) Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants. Plant Cell Tiss Org Cult 45:72–82. doi:10.1007/s11627-008-9164-8

    Google Scholar 

  • Harry IS, Thrope TA (1994) In vitro culture of forest trees. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 539–560

    Google Scholar 

  • Hartmann HT, Kester DE, Davies FT, Geneve R (2010) Plant propagation: principles and practices. Prentice Hall, Upper Saddle River, p 799

    Google Scholar 

  • Herr JM (1971) A new clearing squash technique for the study of ovule development in angiosperms. Am J Bot 58:785–790

    Article  Google Scholar 

  • Hewood VH (1993) Flowering plants of the world. Batsford, BT Londres

    Google Scholar 

  • Hiregoudar LV, Kumar AH, Murthy HN (2005) In vitro culture of Feronia limonia (L.) Swingle from hypocotyl and internodal explants. Biol Plant 49(1):41–45

    Article  Google Scholar 

  • Hiregoudar LV, Murthy HN, Bhat JG, Nayeem A, Hema BP, Hahn EJ, Paek KY (2006) Rapid clonal propagation of Vitex trifolia. Biol Plant 50:291–294

    Article  Google Scholar 

  • Hohtola A (1988) Seasonal changes in explant viability and contamination of tissue cultures from mature Scots pine. Plant Cell Tissue Organ Cult 15:211–222

    Article  Google Scholar 

  • Hong Y, Bhatnagar S (2007) Tropical tree legumes. In: Pua EC, Davey MR (eds) Biotechnology in agriculture and forestry, vol 60. Springer, Berlin, pp 407–431

    Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119

    Article  CAS  Google Scholar 

  • Hussain TM, Chandrasekhar T, Gopal GR (2008) Micropropagation of Sterculia urens Roxb, an endangered tree species from intact seedlings. Afr J Biotechnol 7(2):95–101

    CAS  Google Scholar 

  • Kanwar K, Bhardwaj A, Deepika R, Sharma DR (2007) Robinia pseudoacacia Linn. Tree Forestry Sci Biotechnol 1(1):74–80

    Google Scholar 

  • Kaur K, Kant U (2000) Clonal propagation of Acacia catechu Wild. by shoot tip culture. Plant Growth Regul 31:143–145

    Article  CAS  Google Scholar 

  • Komalavalli N, Rao MV (2000) In vitro micropropagation of Gymnema sylvestre–a multipurpose medicinal plant. Plant Cell Tissue Org Cult 61:97–105. doi:10.1023/A:1006421228598

    Google Scholar 

  • Kramer K, Haänninen H (2009) The annual cycle of development of trees and process-based modelling of growth to scale up from the tree to the stand. In: Noormets A (ed) Phenology of ecosystem processes. Springer Science + Business Media, New York, pp 201–227

    Chapter  Google Scholar 

  • Kumar R, Sharma K, Agrawal V (2005) In vitro clonal propagation of Holarrhena antidysenterica (L.) Wall. through nodal explants from mature trees. In Vitro Cell Dev Biol Plant 41:137–144

    Article  CAS  Google Scholar 

  • Label P, Maldiney R, Sossountzov L, Cornu D, Miginiac E (1988) Endogenous levels of abscisic acid, indole-3-acetic acid and benzyladenine during in vitro bud growth induction of Wild Cherry (Prunus avium L.). Plant Growth Regul 7:171–180

    CAS  Google Scholar 

  • Lambers H et al (2008) Life cycles: environmental influences and adaptations. In: Plant physiological ecology, 2nd edn. Springer Science. Business Media, LLC, New York, pp 375–402

    Google Scholar 

  • Leadem CL (1987) The role of plant growth regulators in the germination of forest tree seeds. Plant Growth Regul 6:61–93

    Article  CAS  Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb Proc Intl Plant Prop Soc 30:421–427

    Google Scholar 

  • Minocha R, Jain SM (2000) Tissue culture of woody plants and its relevance to molecular biology. In: Jain SM, Minocha SC (eds) Molecular biology of woody plants, vol 1. Kluwer, Dodrecht, pp 315–339

    Chapter  Google Scholar 

  • Mostafa SE, Karam NS, Shibli RA, Alali FQ (2010) Micropropagation and production of arbutin in oriental strawberry tree (Arbutus andrachne L). Plant Cell Tissue Organ Cult 103:111–121

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–197

    Article  CAS  Google Scholar 

  • Nikolaeva MG, Razumova MV, Gladkova VN (1985) Guide on the germination of dormant seeds. Nauka Publ, Leningrad (in Russian)

    Google Scholar 

  • Parveen S, Shahzad A, Saema S (2010) In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance. Agrofor Syst 80:109–116

    Article  Google Scholar 

  • Pattnaik SK, Sahoo Y, Chand PK (1996) Micropropagation of a fruit tree, Morus australis Poir. syn. M. acidosa Griff. Plant Cell Rep 15:841–845

    Article  CAS  PubMed  Google Scholar 

  • Peternel Š, Gabrovšek K, Gogala N, Regvar M (2009) In vitro propagation of European aspen (Populus tremula L.) from axillary buds via organogenesis. Hort Sci 121:109–112

    Article  CAS  Google Scholar 

  • Polle A, Rennenberg H (1994) Photooxidative stress in plants: causes and amelioration. In: Foyer C, Mullineaux P (eds) Photo-oxidative stresses in trees. CRC Press, Boca Raton, pp 199–218

    Google Scholar 

  • Rajeswari V, Paliwal K (2008) In vitro shoot organogenesis and plant regeneration from seedling explants of Albizia odoratissima L.f. (Benth.). In Vitro Cell Dev Biol Plant 44:78–83

    Article  CAS  Google Scholar 

  • Ray A, Bhattacharya S (2008) An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride. Plant Cell Tissue Organ Cult 92:315–319

    Article  CAS  Google Scholar 

  • Romano A, Barros S, Martins-Loução MA (2002) Micropropagation of the Mediterranean tree Ceratonia siliqua. Plant Cell Tissue Organ Cult 68:35–41

    Article  CAS  Google Scholar 

  • Sharma PK, Tyagi P, Sharma KC, Kothari SL (2003) Clonal micropropagation of Crataeva adansonii (Dc) Prodr.: a multipurpose tree. In Vitro Cell Dev Biol Plant 39:156–160

    Article  Google Scholar 

  • Sinha RK, Majumdar K, Sinha S (2000) In vitro differentiation and plant regeneration of Albizia chinensis (OBS.) MERR. In Vitro Cell Dev Biol Plant 36:370–373

    Article  CAS  Google Scholar 

  • Stevens ME, Pijut PM (2012) Hypocotyl derived in vitro regeneration of pumpkin ash (Fraxinus profunda). Plant Cell Tissue Organ Cult 108:129–135

    Article  CAS  Google Scholar 

  • Szentesi A, Wink M (1991) Fate of quinolizidine alkaloids through three trophic levels: Laburnum anagyroides (Leguminosae) and associated organisms. J Chem Ecol 17(8):1557–1573

    Article  CAS  PubMed  Google Scholar 

  • Thakar J, Bhargava S (1999) Seasonal variation in antioxidant enzymes and the sprouting response of Gmelina arborea Roxb. nodal sectors cultured in vitro. Plant Cell Tissue Organ Cult 59:181–187

    Article  Google Scholar 

  • Thara G, Dey M, Sahoo L, Panda SK (2011) An insight into the drought stress induced alterations in plants. Biol Plant 55(4):603–613

    Article  Google Scholar 

  • Tutka P, Zatoński W (2006) Cytisine for the treatment of nicotine addition: from a molecule to therapeutic efficacy. Pharmacol Rep 58(6):777–798

    CAS  PubMed  Google Scholar 

  • Tzancova V, Danchev N (2007) Cytisine – from ethomedical use to the development as a natural alternative for smoking cessation. Pharm Biotechnol 21(2):151–160

    Google Scholar 

  • Vengadesan G, Ganapathi A, Amutha S, Selvaraj N (2002) In vitro propagation of Acacia species: a review. Plant Sci 163:663–671

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Botanical Garden, Saratov State University, Navashina str., 1, Saratov, 410010, Russia

    S. N. Timofeeva

  2. Department of Biotechnology, Agricultural Research Institute of South-East Region, Tulaikov str., 7, Saratov, 410010, Russia

    L. A. Elkonin

  3. Department of Genetics, Saratov State University, Astrakhanskaya str., 83, Saratov, 410012, Russia

    O. I. Yudakova & V. S. Tyrnov

Authors
  1. S. N. Timofeeva
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  2. L. A. Elkonin
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  3. O. I. Yudakova
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  4. V. S. Tyrnov
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Correspondence to L. A. Elkonin .

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Editors and Affiliations

  1. Plant Biotechnology Laboratory Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Mohammad Anis

  2. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Naseem Ahmad

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Timofeeva, S.N., Elkonin, L.A., Yudakova, O.I., Tyrnov, V.S. (2016). Application of Tissue Culture for Laburnum anagyroides Medik. Propagation. In: Anis, M., Ahmad, N. (eds) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_8

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