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In Vitro Plant Regeneration in Dainty Spur [Rhinacanthus nasutus (L.) Kurz.] by Organogenesis

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Plant Tissue Culture: Propagation, Conservation and Crop Improvement

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Abstract

A proficient organogenesis protocol was standardised for Rhinacanthus nasutus, a potential medicinal plant. MS medium supplemented with NAA (2.0 mg/l) + ascorbic acid (30 mg/l) was found to be more effective for callus induction. The highest number of 140.7 shoots/explant with shoot length of 15.5 cm from the callus derived from internode explant and 122.3 shoots/explant with shoot length of 11.0 cm from leaf explant-derived callus were observed on MS medium containing 2.0 mg/l BA + 2.0 mg/l KN + 2.0 mg/l NAA. IAA (1.0 mg/l) has showed effective rooting.

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Authors and Affiliations

  1. Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    T. Gouthaman & M. V. Rao

  2. PG & Research Department of Botany, Government Arts College (M), Krishnagiri, 635 001, Tamil Nadu, India

    T. Gouthaman

  3. Department of Industry University Collaboration, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    T. Senthil Kumar

  4. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    A. S. Rao

Authors
  1. T. Gouthaman
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  2. T. Senthil Kumar
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  3. A. S. Rao
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  4. M. V. Rao
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Corresponding author

Correspondence to M. V. Rao .

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Editors and Affiliations

  1. Plant Biotechnology Laboratory Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Mohammad Anis

  2. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Naseem Ahmad

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Gouthaman, T., Senthil Kumar, T., Rao, A.S., Rao, M.V. (2016). In Vitro Plant Regeneration in Dainty Spur [Rhinacanthus nasutus (L.) Kurz.] by Organogenesis. In: Anis, M., Ahmad, N. (eds) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_7

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