Abstract
In this study, culture medium was optimized for economic production of biosurfactant by Anoxybacillus sp. using different waste frying oil, sodium nitrate, and sodium chloride concentrations. Screening step was performed using the Design-Expert software (2 level full factorial design). The response variables are of value for surface tension reduction in the cell-free-culture medium as it indicates the biosurfactant production. The yield of biosurfactant was found to be the highest when surface tension was at the lowest value (42.30 mN/m) at a temperature of 55 °C, agitation 130 rpm, 9 % (v/v) waste frying oil (WFO), 0.5 % (w/v) sodium nitrate (NaNO3), and 0.02 % (w/v) of sodium chloride (NaCl). The biosurfactant was observed to stable in the face of exposure to extreme temperature changes, pH conditions, and salinity. These physiochemical properties demonstrate the potential for using waste frying oil as an inexpensive material for biosurfactant production.
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Acknowledgments
We are greatly indebted to Research Management Institute, (RMI), Universiti Teknologi MARA Shah Alam through Grant 600-RMI/DANA 5/3/RIF (41/2012).
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Khairuddin, N.F., Mulok, T.E.T.Z., Khalil, K.A., Omar, W.S.A.W., Saleh, S.H. (2016). Screening of Medium with Different Range of Waste Frying Oil (WFO), Sodium Nitrate (NaNO3) and Sodium Chloride (NaCl) for Biosurfactant Production by Thermophilic Anoxybacillus sp. Using Fractional Factorial Design (FFD). In: Yusoff, M., Hamid, N., Arshad, M., Arshad, A., Ridzuan, A., Awang, H. (eds) InCIEC 2015. Springer, Singapore. https://doi.org/10.1007/978-981-10-0155-0_2
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DOI: https://doi.org/10.1007/978-981-10-0155-0_2
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