Abstract
With a computer, cloning strategy can be efficiently designed, and REs (Restriction endonucleases) can be easily selected for plasmid fingerprinting. Of course, this can be carried out manually, but computer can make your life easier.
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Notes
- 1.
When performing in vitro transcription using SP6, T7 or T3 RNA polymerase, the template DNA should be linearized in such a way that it has 5′ overhangs. The template DNA with 3′ overhangs could interfere with termination reaction of RNA polymerase.
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Choi, SY., Ro, H., Yi, H. (2019). A Prerequisite for Cloning. In: DNA Cloning: A Hands-on Approach. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1662-6_2
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DOI: https://doi.org/10.1007/978-94-024-1662-6_2
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