Abstract
Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry is an important molecular detection technology due to its extensive mass range, sensitivity, rapid analysis time, and minimal sample preparation. However, quantitative MALDI-TOF approaches have historically suffered from poor accuracy and precision, mainly due to the variability in analyte distribution and a non-uniform sample surface and analyte suppression [1-5]. In order to alleviate spectral complexity at low m/z and ensure chemical specificity, tandem mass spectrometry (MS/MS) is often employed. Herein, we demonstrate that multiple MS/MS events can be performed in a single laser shot [8,9]. This allows the reference of analyte instensity to that of an internal standard in each laser shot, thereby improving accuracy, precision, and throughput.
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Prentice, B.M., Chumbley, C.W., Hachey, B.C., Norris, J.L., Caprioli, R.M. (2017). The Use of Multiple Fragmentation Events in a Single Laser Shot for Improved Drug Quantification by MALDI TOF/TOF Mass Spectrometry. In: Banoub, J., Caprioli, R. (eds) Molecular Technologies for Detection of Chemical and Biological Agents. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1113-3_16
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DOI: https://doi.org/10.1007/978-94-024-1113-3_16
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