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Methods to Measure Water Permeability

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Aquaporins

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 969))

Abstract

Water permeability is a key feature of the cell plasma membranes and it has seminal importance for a number of cell functions such as cell volume regulation, cell proliferation, cell migration, and angiogenesis to name a few. The transport of water occurs mainly through plasma membrane water channels , the aquaporins, who have very important function in physiological and pathophysiological states. Due to the above the experimental assessment of the water permeability of cells and tissues is necessary. The development of new methodologies of measuring water permeability is a vibrant scientific field that constantly develops during the past three decades along with the advances in imaging mainly. In this chapter we describe and critically assess several methods that have been developed for the measurement of water permeability both in living cells as well as in tissues with a focus in the first category.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, SB RAS, 630090, Novosibirsk, Russia

    Evgeniy I. Solenov Ph.D., Galina S. Baturina Ph.D. & Liubov E. Katkova Ph.D.

  2. Novosibirsk State University, 630090, Novosibirsk, Russia

    Evgeniy I. Solenov Ph.D.

  3. Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, 41500, Larissa, Greece

    Sotirios G. Zarogiannis Ph.D.

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  1. Evgeniy I. Solenov Ph.D.
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  2. Galina S. Baturina Ph.D.
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  3. Liubov E. Katkova Ph.D.
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  4. Sotirios G. Zarogiannis Ph.D.
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Correspondence to Evgeniy I. Solenov Ph.D. .

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  1. Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing, China

    Baoxue Yang

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Solenov, E.I., Baturina, G.S., Katkova, L.E., Zarogiannis, S.G. (2017). Methods to Measure Water Permeability. In: Yang, B. (eds) Aquaporins. Advances in Experimental Medicine and Biology, vol 969. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1057-0_18

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