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HEV Cell Culture

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Book cover Hepatitis E Virus

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 948))

Abstract

Cell culture is an important research method in virology. Although many attempts were tried to culture HEV in cells, only two cell culture systems were considered to have high enough efficient for usage. Concentration of virus stocks, host cells, and medium components affect the culture efficient, and the genetic mutations during HEV passage were found to be associated with the increased virulence in cell culture. As an alternative method for traditional cell culture, the infectious cDNA clones were constructed. The viral thermal stability, factors that impact the host range, posttranslation of viral proteins and function of different viral protein were studied using the infectious cDNA clones. The studies on progeny virus showed that the virus secreted from host cells have an envelope, and its formation was associated with pORF3. This result explained the phenomenon that virus could infect hosts cells in the presence of anti-HEV antibodies.

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Abbreviations

Asn:

Asparagine

CPE:

Cytopathetic effect

DMEM:

Dulbecco’s modified eagle medium

FFU:

Fluorescence Forming Unit

HEV:

Hepatitis E virus

Hel:

Helicase

HIV:

Human immunodeficiency virus

HVR:

Hypervariable region

JR:

Joint region

mAb:

Monoclonal antibody

MEM:

Minimum essential medium

Met:

Methyl-transferase

M199:

Medium 199

NCRs:

Non-coding regions

ORF:

Open reading frame

Pro:

Proline

pORFn:

ORFn protein

RdRp:

RNA dependent RNA polymerase

Ser:

Serine

TCID:

Tissue culture infective dose

Thr:

Threonine

WB:

Western blot

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Author information

Authors and Affiliations

  1. Division of Monoclonal Antibody, National Institutes for Food and Drug Control, No. 2 Tiantanxili, Dongcheng District, Beijing, 100050, China

    Feng Zhang Ph.D.

  2. Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control, No. 2 Tiantanxili, Dongcheng District, Beijing, 100050, China

    Youchun Wang M.D., Ph.D.

Authors
  1. Feng Zhang Ph.D.
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  2. Youchun Wang M.D., Ph.D.
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Correspondence to Youchun Wang M.D., Ph.D. .

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Editors and Affiliations

  1. Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing, China

    Youchun Wang

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Zhang, F., Wang, Y. (2016). HEV Cell Culture. In: Wang, Y. (eds) Hepatitis E Virus. Advances in Experimental Medicine and Biology, vol 948. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-0942-0_8

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