Abstract
Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few tens MPa decreases the growth rate and a few hundred MPa decreases the cellular viability. To find clues to understand how such pressure effects on living cells relating to damages on protein molecules, we employed yeast DNA microarrays and analyzed genome-wide gene-expression levels in yeast cells which have been exposed to different levels of hydrostatic pressure. These include the cells temporarily adapted to a high pressure (from 0.1 to 30 MPa) and to a low pressure (from 30 to 0.1 MPa). These conditions cause yeast cells decreases of growth rate. We also analyzed gene expression profiles from the cells recovering after the sublethal pressure treatment at 180 MPa at 4 °C for 0 min and at 40 MPa at 4 °C for 16 h. These conditions cause yeast cells decreases of cellular viability. The activated genes by the temporary adaptations to both of the high pressure and the low pressure suggest that proteins related to membrane biosynthesis and cell wall biosynthesis can be crucial targets of pressure-induced damages, whereas the activated genes under recovering conditions after exposure to the sublethal high pressure suggest that proteasome activity and proteins localized in endoplasmic reticulum can be the crucial targets or the essential factors to survive.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abe F (2004) Piezophysiology of yeast: occurrence and significance. Cell Mol Biol 50:437–445
Abe F, Horikoshi K (1995) Hydrostatic pressure promotes the acidification of vacuoles in Saccharomyces cerevisiae. FEMS Microbiol Lett 130:307–312
Abe F, Horikoshi K (2000) Tryptophan permease gene TAT2 confers high-pressure growth in Saccharomyces cerevisiae. Mol Cell Biol 20:8093–8102
Brown SL, Stockdale VJ, Pettolino F, Pocock KF, de Barros Lopes M, Williams PJ, Bacic A, Fincher GB, Høj PB, Waters EJ (2007) Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w. Appl Microbiol Biotechnol 73:1363–1376
Culbertson MR, Henry SA (1975) Inositol-requiring mutants of Saccharomyces cerevisiae. Genetics 80:23–40
Iwahashi H, Obuchi K, Kaul SC, Komatsu Y (1991) Induction of barotolerance by heat shock treatment in yeast. FEMS Microbiol Lett 80:325–328
Iwahashi H, Obuchi K, Fujii S, Komatsu Y (1997a) Barotolerance is dependent on both trehalose and hsp104 but essentially different from thermotolerance in Saccharomyces cerevisiae. Lett Appl Microbiol 25:43–47
Iwahashi H, Obuchi K, Fujii S, Komatsu Y (1997b) Effect of temperature on the role of Hsp104 and trehalose in barotolerance of Saccharomyces cerevisiae. FEBS Lett 416:1–5
Iwahashi H, Nwaka S, Obuchi K, Komatsu Y (1998) Evidence for the interplay between trehalose metabolism and heat shock proteins 104 in yeast. Appl Environ Microbiol 64:4614–4617
Iwahashi H, Shimizu H, Odani M, Komatsu Y (2003a) Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae. Extremophile 7:291–298
Iwahashi H, Ishidou E, Odani M, Homma T, Oka S (2003b) Low pressure shock response of yeast. In: Winter R (ed) Advances in high pressure bioscience and biotechnology. Springer, Heidelberg, pp 275–278
Iwahashi H, Odani M, Ishidou E, Kitagawa E (2005) Adaptation of Saccharomyces cerevisiae to high hydrostatic pressure causing growth inhibition. FEBS Lett 579:2847–2852
Kitagawa E, Momose Y, Iwahashi H (2001) Correlation of the structures of agricultural fungicides to gene expression in Saccharomyces cerevisiae upon exposure to toxic doses. Environ Sci Tech 15:2788–2793
Kitagawa E, Momose Y, Iwahashi H (2003) Correlation of the structures of agricultural fungicides to gene expression in Saccharomyces cerevisiae upon exposure to toxic doses. Environ Sci Tech 15:2788–2793
Kodaki T, Yamashita S (1987) Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J Biol Chem 262:15428–15435
Koseki S, Yamamoto K (2006) Recovery of Escherichia coli ATCC 25922 in phosphate buffered saline after treatment with high hydrostatic pressure. Int J Food Microbiol 110:108–111
Masselot M, De Robichon-Szulmajster H (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139:121–132
Mattison CP, Spencer SS, Kresge KA, Lee J, Ota IM (1999) Differential regulation of the cell wall integrity mitogen-activated protein kinase pathway in budding yeast by the protein tyrosine phosphatases Ptp2 and Ptp3. Mol Cell Biol 19:7651–7660
Momose Y, Iwahashi H (2001) Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium. Environ Toxicol Chem 20:2353–2360
Murata Y, Watanabe T, Sato M, Momose Y, Nakahara T, Oka S, Iwahashi H (2003) DMSO exposure facilitates phospholipid biosynthesis and cellular membrane proliferation in yeast cells. J Biol Chem 278:33185–33193
Ohshima S, Nomura K, Iwahashi H (2013) Clarification of the recovery mechanism of Escherichia coli after hydrostatic pressure treatment. High Pressure Res 33:308–314
O’Reilly CE, O’Connor PM, Kelly AL, Beresford TP, Murphy PM (2000) Use of hydrostatic pressure for inactivation of microbial contaminants in cheese. Appl Environ Microbiol 66:4890–4896
Osumi M, Sato M, Kobori H, Zha Hai feng, Ishijima AS, Hamada K, Shimada S (1996) Morphological effect of pressure stress on yeast. In: Hayashi R, Balony C (eds) High pressure bioscience and biotechnology. Elsevier, Amsterdam, pp 37–46
Sonoike K, Setoyama T, Kuma Y, Shinno T, Fukumoto K, Ishihara M (1993) Effects of pressure and temperature on the death rate of Lactobacillus casei and Escherichia coli. In: Hayashi R (ed) High pressure bioscience and food science. Sanei Press, Kyoto, pp 213–219
Tamura K, Shimizu T, Kourai H (1992) Effects of ethanol on the growth and elongation of Escherichia coli under high pressures up to 40 MPa. FEMS Microbiol Lett 78:321–324
Tanaka Y, Higashi T, Rakwal R, Wakida S, Iwahashi H (2008) Development of a capillary electrophoresis-mass spectrometry method using polymer capillaries for metabolomic analysis of yeast. Electrophoresis 29:2016–2023
Tanaka Y, Higashi T, Rakwal R, Shibato J, Wakida S, Iwahashi H (2010) The role of proteasome in yeast Saccharomyces cerevisiae response to sublethal high-pressure treatment. High Pres Res 30:519–523
Tsukada M, Ohsumi Y (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333:169–174
Yayanos AA, Pollard EC (1969) A study of the effects of hydrostatic pressure on macromolecular synthesis in Escherichia coli. Biophys J 9:1464–1482
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Iwahashi, H. (2015). Pressure-Dependent Gene Activation in Yeast Cells. In: Akasaka, K., Matsuki, H. (eds) High Pressure Bioscience. Subcellular Biochemistry, vol 72. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9918-8_20
Download citation
DOI: https://doi.org/10.1007/978-94-017-9918-8_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-9917-1
Online ISBN: 978-94-017-9918-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)