Abstract
There are no two identical cells in any organism and with human more so. Quantitative measurements of functions in single cell is a critical challenge in any modern biological study. Now with the development of single cell isolation technology, the era of single cell based research is coming, especially when combination with omics science, which takes full advantage of both techniques, and gets rid of their disadvantages. There are several methods for single cell isolation: serial dilution, micromanipulation (mechanical-using a simple mouth pipette and optimal), fluorescence-activated cell sorting (FACS), laser-capture microdissection (LCM) and microfluidic devices. But some techniques need very expensive equipments, which is not easily available for most investigators. Here we developed a simple protocol to isolate and culture single lung cancer cell. This will make single cell-based lung cancer research much more available for individual investigator.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Brehm-Stecher B, Johnson EA. Single-cell microbiology: tools, technologies, and applications [J]. Microbiol Mol Biol Rev. 2004;68(3):538–59.
Button D, Schut F, Quang P, et al. Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results [J]. Appl Environ Microbiol. 1993;59(3):881–91.
Connon SA, Giovannoni SJ. High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates [J]. Appl Environ Microbiol. 2002;68(8):3878–85.
Frumkin D, Wasserstrom A, Kaplan S, et al. Genomic variability within an organism exposes its cell lineage tree [J]. PLoS Comput Biol. 2005;1(5):e50.
Kurimoto K, Yabuta Y, Ohinata Y, et al. Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis [J]. Nat Protoc. 2007;2(3):739–52.
Reizel Y, Chapal-Ilani N, Adar R, et al. Colon stem cell and crypt dynamics exposed by cell lineage reconstruction [J]. PLoS Genet. 2011;7(7):e1002192.
Schut F, de Vries EJ, Gottschal JC, et al. Isolation of typical marine bacteria by dilution culture: growth, maintenance, and characteristics of isolates under laboratory conditions [J]. Appl Environ Microbiol. 1993;59(7):2150–60.
Seo J, Park S-J, Kim J, et al. Effective method for the isolation and proliferation of primary lung cancer cells from patient lung tissues [J]. Biotechnol Lett. 2013;35(8):1165–74.
Shlush LI, Chapal-Ilani N, Adar R, et al. Cell lineage analysis of acute leukemia relapse uncovers the role of replication-rate heterogeneity and microsatellite instability [J]. Blood. 2012;120(3):603–12.
Zong C, Lu S, Chapman AR, et al. Genome-wide detection of single-nucleotide and copy-number variations of a single human cell [J]. Science. 2012;338(6114):1622–6.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Zhang, D., Wang, X. (2015). A Simple Protocol for Single Lung Cancer Cell Isolation-Making the Single Cell Based Lung Cancer Research Feasible for Individual Investigator. In: Wang, X. (eds) Single Cell Sequencing and Systems Immunology. Translational Bioinformatics, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9753-5_12
Download citation
DOI: https://doi.org/10.1007/978-94-017-9753-5_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-9752-8
Online ISBN: 978-94-017-9753-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)