Abstract
In this chapter, we describe the use of an automated platform for live imaging and monitoring of endothelial cell angiogenesis. Using this system, movement and orientation can be monitored in normal or manipulated experimental conditions including hypoxia. In addition, cell labelling techniques can be used to visualise cell orientation, movement and signalling. A description of the current technology is provided as well as a trouble-shooting section.
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References
Huttunen TT, Sundberg M et al (2011) An automated continuous monitoring system: a useful tool for monitoring neuronal differentiation of human embryonic stem cells. Stem Cell Stud 1(e10):71–77
Mikkola M, Toivonen S et al (2013) Lectin from Erythrina cristagalli supports undifferentiated growth and differentiation of human pluripotent stem cells. Stem Cells Dev 22(5):707–716
Toivonen S, Ojala M et al (2013) Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines. Stem Cell Trans. doi: 10.5966/sctm.2012-0047
Vuoristo S, Toivonen S et al (2013) A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation. PLoS One 8(10):e76205
Kivi N, Greco D et al (2008) Genes involved in cell adhesion, cell motility and mitogenic signalling are altered due to HPV 16 ES protein expression. Oncogene 27:2532–2541
Talmana V, Amadiob M et al (2013) The C1 domain-targeted isophthalate derivative HMI-1b11 promotes neurite outgrowth and GAP-43 expression through PKC activation in SH-SY5Y cells. Pharmacol Res 73:44–54
Palazzolo G, Horvath P et al (2012) The flavonoid isoquercitrin promotes neurite outgrowth by reducing Rho A activity. PLoS One 7(11):e49979
Talman V, Tuominen RK et al (2011) C1 Domain-targeted isophthalate derivatives induce cell elongation and cell cycle arrest in HeLa cells. PLoS One 6(5):e20053. doi:10.1371/journal.pone.0020053
Bosutti A, Qui J et al (2013) Targeting p35/cdk5 signalling via CIP-peptides promotes angiogenesis in hypoxia. PLoS One 8(9):e75538
Ball SG, Worthington JJ et al (2013) Mesenchymal stromal cells: inhibiting PDGF receptors or depleting fibronectin induces mesodermal progenitors with endothelial potential. Stem Cells 10. doi: 10.1002/stem.1538
Sonka M, Hlavac V et al (1999) Image processing, analysis, and machine vision, 2nd edn. PWS Pub, Pacific Grove
Tarvainen J, Saarinen M et al (2002) Creating images with high data contents for microworld applications. Ind Sys Rev:17–23
Tozer GM, Akerman S et al (2008) Blood vessel maturation and response to vascular-disrupting therapy in single vascular endothelial growth factor-A isoform–producing tumors. Cancer Res 68(7):2301–2311
Walter MNM, Wright KT et al (2010) Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res 316(7):1271–1281
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Bray, E., Slevin, M. (2015). Evaluating In Vitro Angiogenesis Using Live Cell Imaging. In: Slevin, M., McDowell, G. (eds) Handbook of Vascular Biology Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9716-0_4
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DOI: https://doi.org/10.1007/978-94-017-9716-0_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-9715-3
Online ISBN: 978-94-017-9716-0
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