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A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood

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Handbook of Vascular Biology Techniques

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Abstract

In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.

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References

  1. Body R, Pemberton P, Ali F, McDowell G, Carley S, Smith A, Mackway-Jones K (2011) Low soluble P-selectin may facilitate early exclusion of acute myocardial infarction. Clin Chim Acta 412:614–618

    Article  CAS  PubMed  Google Scholar 

  2. McDowell G, Temple I, Li C, Kirwan CC, Bundred NJ, McCollum CN, Burton IE, Kumar S, Byrne GJ (2005) Alteration in platelet function in patients with early breast cancer. Anticancer Res 25:3963–3966

    CAS  PubMed  Google Scholar 

  3. Byrne GJ, McDowell G, Agarawal R, Sinha G, Kumar S, Bundred NJ (2007) Serum vascular endothelial growth factor in breast cancer. Anticancer Res 27:3481–3487

    CAS  PubMed  Google Scholar 

  4. Kirwan CC, McDowell G, McCollum CN, Kumar S, Byrne GJ (2008) Early changes in the haemostatic and procoagulant systems after chemotherapy in breast cancer. Br J Cancer 99:1000–1006

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. School of Healthcare Science, Manchester Metropolitan University, Manchester, UK

    Garry McDowell Ph.D., F.R.C.Path., F.E.S.C. & Mark Slevin Ph.D., F.R.C.Path., F.A.H.A.

  2. Institute of Cardiovascular Science, University of Manchester, Manchester, UK

    Richard Body Ph.D., F.C.E.M.

  3. Department of Academic Surgery, University of Manchester and University Hospital of South Manchester, Manchester, UK

    Cliona Kirwan Ph.D., F.R.C.S. & Ged Byrne M.D., F.R.C.S.

Authors
  1. Garry McDowell Ph.D., F.R.C.Path., F.E.S.C.
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  2. Richard Body Ph.D., F.C.E.M.
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  3. Cliona Kirwan Ph.D., F.R.C.S.
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  4. Ged Byrne M.D., F.R.C.S.
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  5. Mark Slevin Ph.D., F.R.C.Path., F.A.H.A.
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Corresponding author

Correspondence to Garry McDowell Ph.D., F.R.C.Path., F.E.S.C. .

Editor information

Editors and Affiliations

  1. School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom

    Mark Slevin

  2. School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom

    Garry McDowell

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McDowell, G., Body, R., Kirwan, C., Byrne, G., Slevin, M. (2015). A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood. In: Slevin, M., McDowell, G. (eds) Handbook of Vascular Biology Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9716-0_35

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