Abstract
The dorsal air sac assay (DASA) is a simple and convenient in vivo assay to measure angiogenesis and angiogenesis inhibition by substances of interest. In the assay, tumor cells that release angiogenic factors are placed in a diffusion chamber that consists of a plastic or rubber ring covered with cellulose membrane filters on both sides. The chamber is implanted into a dorsal air sac under the skin of mice. Angiogenic factors released from the tumor cells induce angiogenesis in the mouse air sac fascia attached to the chamber. The newly formed blood vessels are readily recognizable and these blood vessels may be quantified. The chamber-bearing mice may be treated with antiangiogenic agents by systemic administration and the degree of the angiogenesis inhibition can be measured and quantified after the mice are sacrificed.
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Abbreviations
- DASA:
-
Dorsal air sac assay
- VEGF:
-
Vascular endothelial growth factor
- mAb:
-
Monoclonal antibody
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© 2015 Springer Science+Business Media Dordrecht
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Seon, B.K. (2015). Dorsal Air Sac Assay. In: Slevin, M., McDowell, G. (eds) Handbook of Vascular Biology Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9716-0_13
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DOI: https://doi.org/10.1007/978-94-017-9716-0_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-9715-3
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