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Methods of Hydrogen Application

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Hydrogen Molecular Biology and Medicine

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Abstract

Hydrogen gas has come to the forefront of therapeutic medical gas research. Recent basic and clinical studies have revealed that hydrogen gas is an important physiological regulatory factor, with antioxidant, anti-inflammatory, and antiapoptotic protective effects on cells and organs. Since hydrogen gas is explosive, the methods of hydrogen application become an important issue for researchers. Currently, the methods of hydrogen application are developing rapidly, including inhalation of hydrogen gas, oral hydrogen water, hydrogen saline injection, eye drops, skin smear and bathing, hydrogen gas injection, etc. In this chapter, various methods of hydrogen application are discussed with particular attention to the experimental models and human beings.

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References

  1. Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007;13(6):688–94.

    Article  CAS  PubMed  Google Scholar 

  2. Lee MY, Kim YK, Ryoo KK, Lee YB, Park EJ. Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein. Appl Biochem Biotechnol. 2006;135(2):133–44.

    Article  CAS  PubMed  Google Scholar 

  3. Ohsawa I, Nishimaki K, Yamagata K, Ishikawa M, Ohta S. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochem Biophys Res Commun. 2008;377(4):1195–8.

    Article  CAS  PubMed  Google Scholar 

  4. Cai J, Kang Z, Liu K, Liu W, Li R, Zhang JH, Luo X, Sun X. Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model. Brain Res. 2009;1256:129–37.

    Article  CAS  PubMed  Google Scholar 

  5. Kajiya M, Sato K, Silva MJ, Ouhara K, Do PM, Shanmugam KT, Kawai T. Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis. Biochem Biophys Res Commun. 2009;386(2):316–21.

    Article  CAS  PubMed  Google Scholar 

  6. Huang G, Zhou J, Zhan W, Xiong Y, Hu C, Li X, Li X, Li Y, Liao X. The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest. Resuscitation. 2013;84(5):690–5.

    Article  CAS  PubMed  Google Scholar 

  7. Ball R. Biochemical decompression of hydrogen by naturally occurring bacterial flora in pigs: what are the implications for human hydrogen diving? Undersea Hyperb Med. 2001;28(2):55–6. (Journal of the Undersea and Hyperbaric Medical Society, Inc.)

    CAS  PubMed  Google Scholar 

  8. Kang KM, Kang YN, Choi IB, Gu Y, Kawamura T, Toyoda Y, Nakao A. Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors. Med Gas Res. 2011;1(1):11.

    Article  PubMed Central  PubMed  Google Scholar 

  9. Qian L, Shen J, Chuai Y, Cai J. Hydrogen as a new class of radioprotective agent. Int J Biol Sci. 2013;9(9):887–94.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Ohta S. Molecular hydrogen as a preventive and therapeutic medical gas: initiation, development and potential of hydrogen medicine. Pharmacol Ther. 2014;144:1–11.

    Article  CAS  PubMed  Google Scholar 

  11. Ohno K, Ito M, Ichihara M, Ito M. Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases. Oxid Med Cell Longev. 2012;2012:353152.

    Article  PubMed Central  PubMed  Google Scholar 

  12. Qian L, Mei K, Shen J, Cai J. Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD). Transplantation. 2013;95(5):658–62.

    Article  CAS  PubMed  Google Scholar 

  13. Qian L, Cao F, Cui J, Huang Y, Zhou X, Liu S, Cai J. Radioprotective effect of hydrogen in cultured cells and mice. Free Radic Res. 2010;44(3):275–82.

    Article  CAS  PubMed  Google Scholar 

  14. Qian L, Cao F, Cui J, Wang Y, Huang Y, Chuai Y, Zaho L, Jiang H, Cai J. The potential cardioprotective effects of hydrogen in irradiated mice. J Radiat Res. 2010;51(6):741–7.

    Article  CAS  PubMed  Google Scholar 

  15. Ono H, Nishijima Y, Adachi N, Sakamoto M, Kudo Y, Nakazawa J, Kaneko K, Nakao A. Hydrogen(H2) treatment for acute erythymatous skin diseases. A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers. Med Gas Res. 2012;2(1):14.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Oharazawa H, Igarashi T, Yokota T, Fujii H, Suzuki H, Machide M, Takahashi H, Ohta S, Ohsawa I. Protection of the retina by rapid diffusion of hydrogen: administration of hydrogen-loaded eye drops in retinal ischemia-reperfusion injury. Invest Ophthalmol Vis Sci. 2010;51(1):487–92.

    Article  PubMed  Google Scholar 

  17. Ignacio RM, Yoon Y-S, Sajo MEJ, Kim C-S, Kim D-H, Kim S-K, Lee K-J. The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice. Mol Cell Toxicol. 2013;9(1):15–21.

    Article  CAS  Google Scholar 

  18. Chen X, Zuo Q, Hai Y, Sun XJ. Lactulose: an indirect antioxidant ameliorating inflammatory bowel disease by increasing hydrogen production. Med Hypotheses. 2011;76(3):325–7.

    Article  CAS  PubMed  Google Scholar 

  19. Liu S, Sun Q, Tao H, Sun X. Oral administration of mannitol may be an effective treatment for ischemia-reperfusion injury. Med Hypotheses. 2010;75(6):620–2.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Hematology, Navy General Hospital, Fucheng Road, Beijing, China

    Liren Qian & Jianliang Shen

  2. Department of Navy Aeromedicine, Second Military Medical University, Shanghai, China

    Xuejun Sun

Authors
  1. Liren Qian
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  2. Jianliang Shen
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  3. Xuejun Sun
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Corresponding author

Correspondence to Xuejun Sun .

Editor information

Editors and Affiliations

  1. Department of Diving Medicine, Second Military Medical University, Shanghai, China

    Xuejun Sun

  2. Department of Biochemistry and Cell Biol, Nippon Medical School, Kawasaki, Japan

    Shigeo Ohta

  3. Department of Emergency, Hyogo College of Medicine, Nishinomiya, Japan

    Atsunori Nakao

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Qian, L., Shen, J., Sun, X. (2015). Methods of Hydrogen Application. In: Sun, X., Ohta, S., Nakao, A. (eds) Hydrogen Molecular Biology and Medicine. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9691-0_7

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