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Variations of Human Urinary Proteome

  • Yong ChenEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 845)

Abstract

The study on dynamic analysis of human urinary proteome is the foundation that we discriminate certain various urinary proteins as potential bio-marker derived from the disease itself or normal physiological change. In our results, based on RPLC-MS/MS and spectral count to study pooled and individual urine samples and other researchers’ studies, it can be known that the content of many urinary proteins maintain relatively stable. We have reason to believe that the relatively stable urinary protein is a very valuable resource as biomarkers. Many similar proteins such as prostaglandin-H2 D-isomerase and apolipoprotein D proteins have been proved our hypothesis. The following field, the number, preservation and treatment methods of urine sample, the standardization of analysis method and data processing, and suitable quantitative method, is ought to the focus of future study.

Keywords

Variation Human urine proteome 

References

  1. 1.
    Adachi J, Kumar C, Zhang Y, Olsen JV, Mann M (2006) The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol 7:R80PubMedCrossRefPubMedCentralGoogle Scholar
  2. 2.
    Fliser D, Novak J, Thongboonkerd V, Argiles A, Jankowski V, Girolami MA, Jankowski J, Mischak H (2007) Advances in urinary proteome analysis and biomarker discovery. J Am Soc Nephrol (JASN) 18:1057–1071CrossRefGoogle Scholar
  3. 3.
    Hirawa N, Uehara Y, Ikeda T, Gomi T, Hamano K, Totsuka Y, Yamakado M, Takagi M, Eguchi N, Oda H, Seiki K, Nakajima H, Urade Y (2001) Urinary prostaglandin D synthase (beta-trace) excretion increases in the early stage of diabetes mellitus. Nephron 87:321–327PubMedCrossRefGoogle Scholar
  4. 4.
    Hirawa N, Uehara Y, Yamakado M, Toya Y, Gomi T, Ikeda T, Eguchi Y, Takagi M, Oda H, Seiki K, Urade Y, Umemura S (2002) Lipocalin-type prostaglandin d synthase in essential hypertension. Hypertension 39:449–454PubMedCrossRefGoogle Scholar
  5. 5.
    Jain S, Rajput A, Kumar Y, Uppuluri N, Arvind AS, Tatu U (2005) Proteomic analysis of urinary protein markers for accurate prediction of diabetic kidney disorder. J Assoc Phys India 53:513–520Google Scholar
  6. 6.
    Kentsis A, Monigatti F, Dorff K, Campagne F, Bachur R, Steen H (2009) Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl 3:1052–1061PubMedCrossRefPubMedCentralGoogle Scholar
  7. 7.
    Molina L, Salvetat N, Ameur RB, Peres S, Sommerer N, Jarraya F, Ayadi H, Molina F, Granier C (2011) Analysis of the variability of human normal urine by 2D-GE reveals a “public” and a “private” proteome. J Proteomics 75:70–80PubMedCrossRefGoogle Scholar
  8. 8.
    Nagaraj N, Mann M (2011) Quantitative analysis of the intra- and inter-individual variability of the normal urinary proteome. J Proteome Res 10:637–645PubMedCrossRefGoogle Scholar
  9. 9.
    Nedelkov D (2005) Population proteomics: addressing protein diversity in humans. Expert Rev Proteomics 2:315–324PubMedCrossRefGoogle Scholar
  10. 10.
    Nedelkov D, Kiernan UA, Niederkofler EE, Tubbs KA, Nelson RW (2006) Population proteomics: the concept, attributes, and potential for cancer biomarker research. Mol Cell Proteomics (MCP) 5:1811–1818CrossRefGoogle Scholar
  11. 11.
    Rodriguez JC, Diaz M, Gonzalez LO, Sanchez J, Sanchez MT, Merino AM, Vizoso F (2000) Apolipoprotein D expression in benign and malignant prostate tissues. Int J Surg Invest 2:319–326Google Scholar
  12. 12.
    Sun W, Chen Y, Li F, Zhang L, Yang R, Zhang Z, Zheng D, Gao Y (2009) Dynamic urinary proteomic analysis reveals stable proteins to be potential biomarkers. Proteomics—Clin Appl 3:370–382CrossRefGoogle Scholar
  13. 13.
    Utsunomiya T, Ogawa K, Yoshinaga K, Ohta M, Yamashita K, Mimori K, Inoue H, Ezaki T, Yoshikawa Y, Mori M (2005) Clinicopathologic and prognostic values of apolipoprotein D alterations in hepatocellular carcinoma. Int J Cancer 116:105–109PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Lanzhou Institute of Biological Products Co., Ltd.LanzhouChina

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