Abstract
Urine is a body fluid that can be noninvasively acquired and contains important biological information about the patient. Urinary proteins are considered to be the best resource of potential biomarkers for kidney disorders. Urinary proteins can be adsorbed to polyvinylidene fluoride (PVDF) or nitrocellulose membranes, which can then be dried and stored in vacuum bag. This membrane is named Urimem. The membrane can even be stored at room temperature for at least weeks without changing the quantity of eluted proteins. With this simple and inexpensive urimem, it is possible to begin preserving urine sample from all consenting patients during each stage of kidney disease development. Thus, the medical research can be conducted more economically, ultimately benefiting the patients who provided the samples. This can potentially change the landscape of medical research and medical practice.
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References
Jia L, Liu X, Liu L, Li M, Gao Y (2014) Urimem, a membrane that can store urinary proteins simply and economically, makes the large-scale storage of clinical samples possible. Sci China Life Sci 57(3):336–339 March 2014
Szewczyk B, Summers DF (1988) Preparative elution of proteins blotted to immobilon membranes. Anal Biochem 168(1):48–53
Nakamura K, Tanaka T, Kuwahara A, Takeo K (1985) Microassay for proteins on nitrocellulose filter using protein dye-staining procedure. Anal Biochem 148:311–319
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© 2015 Springer Science+Business Media Dordrecht
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Liu, L., Jia, L., Liu, X. (2015). Urimem Facilitates Kidney Disease Biomarker Research. In: Gao, Y. (eds) Urine Proteomics in Kidney Disease Biomarker Discovery. Advances in Experimental Medicine and Biology, vol 845. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9523-4_3
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DOI: https://doi.org/10.1007/978-94-017-9523-4_3
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