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Urimem Facilitates Kidney Disease Biomarker Research

  • Liu LiuEmail author
  • Lulu Jia
  • Xuejiao Liu
Chapter
  • 1.8k Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 845)

Abstract

Urine is a body fluid that can be noninvasively acquired and contains important biological information about the patient. Urinary proteins are considered to be the best resource of potential biomarkers for kidney disorders. Urinary proteins can be adsorbed to polyvinylidene fluoride (PVDF) or nitrocellulose membranes, which can then be dried and stored in vacuum bag. This membrane is named Urimem. The membrane can even be stored at room temperature for at least weeks without changing the quantity of eluted proteins. With this simple and inexpensive urimem, it is possible to begin preserving urine sample from all consenting patients during each stage of kidney disease development. Thus, the medical research can be conducted more economically, ultimately benefiting the patients who provided the samples. This can potentially change the landscape of medical research and medical practice.

Keywords

Urimem Storage Urinary proteins 

References

  1. 1.
    Jia L, Liu X, Liu L, Li M, Gao Y (2014) Urimem, a membrane that can store urinary proteins simply and economically, makes the large-scale storage of clinical samples possible. Sci China Life Sci 57(3):336–339 March 2014PubMedCrossRefGoogle Scholar
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    Szewczyk B, Summers DF (1988) Preparative elution of proteins blotted to immobilon membranes. Anal Biochem 168(1):48–53PubMedCrossRefGoogle Scholar
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    Nakamura K, Tanaka T, Kuwahara A, Takeo K (1985) Microassay for proteins on nitrocellulose filter using protein dye-staining procedure. Anal Biochem 148:311–319Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Key Laboratory of Medical Molecular Biology, Department of PathophysiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic MedicineBeijingChina
  2. 2.Department of PharmacyBeijing Children’s Hospital, Capital Medical UniversityBeijingChina
  3. 3.Department of NephrologyBeijing Anzhen Hospital, Capital Medical UniversityChaoyang District, BeijingChina
  4. 4.Department of NephrologyPeking Union Medical College HospitalDongcheng District, BeijingChina

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