Effects of Exercise on the Urinary Proteome

  • Maxie Kohler
  • Wilhelm Schänzer
  • Mario ThevisEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 845)


Exercise-induced proteinuria has been observed and studied for more than a century. It was found that different sport disciplines alter the urinary proteome in different ways. Moderate-intensity exercise results in increased glomerular filtration, meaning that medium-sized proteins are excreted in higher amounts, while high-intensity exercise of short duration also increases the excretion of low molecular weight proteins as a result of tubular dysfunction. Exhaustive exercise may lead to the excretion of hemoglobin or myoglobin, which changes the urinary proteome considerably. Studies comparing protein maps of different sport types compared to a control group showed that quality and quantity of urinary proteins are interindividually different. In addition, urine samples collected before and after exercise exhibit substantially different protein patterns even from the same person. Therefore, further studies investigating the urinary proteome are desirable. As the variation of protein content and composition in urine are generally much higher than in other matrices, respective studies need to be well controlled and homogenous groups of volunteers should be chosen. In addition to the sport-related physiological and biochemical interest, exercise-induced protein changes also need to be considered for biomarker measurements from urine samples for kidney or other diseases.


Urinary proteome Exercise Biomarker Kidney diseases 



The study was conducted with support of the Manfred-Donike-Institute, Cologne, Germany, and Antidoping Switzerland, Berne, Switzerland.


  1. 1.
    Abián-Vicén J, Del Coso J, González-Millán C et al (2012) Analysis of dehydration and strength in elite badminton players. PLoS ONE 7:e37821PubMedPubMedCentralCrossRefGoogle Scholar
  2. 2.
    Adachi J, Kumar C, Zhang Y et al (2006) The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol 7:R80PubMedPubMedCentralCrossRefGoogle Scholar
  3. 3.
    Bellinghieri G, Savica V, Santoro D (2008) Renal alterations during exercise. J Ren Nutr 18:158–164PubMedCrossRefGoogle Scholar
  4. 4.
    Collier W, Lond F (1907) Functional albuminuria in athletes. Br Med J 1:4–6PubMedPubMedCentralCrossRefGoogle Scholar
  5. 5.
    Dunhill TP, Patterson SW (1902) Albuminuria following severe exercise in healthy persons. Intercol Med J Australia 2:334Google Scholar
  6. 6.
    Gündüz F, Kuru O, Şentürk ÜK (2005) Angiotensin II inhibition attenuates postexercise proteinuria in rats. Int J Sports Med 26:710–713PubMedCrossRefGoogle Scholar
  7. 7.
    Gür H, Küçükoğlu S (1994) Effects of age, training background and duration of running on abnormal urinary findings after a half-marathon race. Br J Sports Med 28:61–62PubMedPubMedCentralCrossRefGoogle Scholar
  8. 8.
    Haraldsson B, Nyström J, Deen W (2008) Properties of the glomerular barrier and mechanisms of proteinuria. Physiol Rev 88:451–487PubMedCrossRefGoogle Scholar
  9. 9.
    Hatcher W, Webb A (1979) Georges Hubert Esbach and the tube albuminometer. Med Lab Sci 36:185–190PubMedGoogle Scholar
  10. 10.
    Heathcote K, Wilson MP, Quest DQ et al (2009) Prevalence and duration of exercise induced albuminuria in healthy people. Clin Invest Med 32:E261–E265PubMedGoogle Scholar
  11. 11.
    Hiraki K, Kamijo-Ikemori A, Yasuda T et al (2013) Moderate-intensity single exercise session does not induce renal damage. J Clin Lab Anal 27:177–180PubMedCrossRefGoogle Scholar
  12. 12.
    Hoorn EJ, Pisitkun T, Zietse R et al (2005) Prospects for urinary proteomics: exosomes as a source of urinary biomarkers. Nephrology 10:283–290PubMedCrossRefGoogle Scholar
  13. 13.
    Houser M (1984) Assessment of proteinuria using random urine samples. J Pediatr 104:5–8CrossRefGoogle Scholar
  14. 14.
    Jones G, Newhouse I (1997) Sport-related hematuria: a review. Clin J Sports Med 7:119–125CrossRefGoogle Scholar
  15. 15.
    Junglee NA, Di Felice U, Dolci A et al (2013) Exercising in a hot environment with muscle damage: effects on acute kidney injury biomarkers and kidney function. Am J Physiol Enal Physiol 305:F813–F820CrossRefGoogle Scholar
  16. 16.
    Kohanpour MA, Sanavi S, Peeri M et al (2012) Kidney diseases effect of submaximal aerobic exercise in hypoxic conditions on proteinuria and hematuria in physically trained young men. Iran J Kidney Dis 6:192–197PubMedGoogle Scholar
  17. 17.
    Kohler M, Franz S, Regeniter A et al (2009) Comparison of the urinary protein patterns of athletes by 2D-gel electrophoresis and mass spectrometry—a pilot study. Drug Test Anal 1:382–386PubMedCrossRefGoogle Scholar
  18. 18.
    Kohler M, Walpurgis K, Thomas A et al (2010) Effects of endurance exercise on the urinary proteome analyzed by 2-D PAGE and Orbitrap MS. Proteomics Clin Appl 4:568–576PubMedGoogle Scholar
  19. 19.
    McCullough PA, Chinnianyan KM, Gllagher MJ et al (2011) Changes in renal markers and acute kidney injury after marathon running. Nephrology 16:194–199PubMedCrossRefGoogle Scholar
  20. 20.
    Mittleman K, Zambraski E (1992) Exercise-induced proteinuria is attenuated by indomethacin. Med Sci Sports Exerc 24:1069–1074PubMedCrossRefGoogle Scholar
  21. 21.
    Montelpare W, Klentrou P (2002) Continuous versus intermittent exercise effects on urinary excretion of albumin and total protein. J Sci Med Sport 5:219–223PubMedCrossRefGoogle Scholar
  22. 22.
    Narloch JA, Brandstater ME (1995) Influence of btreathing technique on arterial blood pressure during heavy weight lifting. Arch Phys Med Rehabil 76:457–462Google Scholar
  23. 23.
    Patel DR, Torres AD, Greydanus DE (2005) Kidneys and sports. Adolesc Med Clin 16:111–119PubMedCrossRefGoogle Scholar
  24. 24.
    Peterson PA, Evriun PE, Berggard I (1969) Differentiation of glomerular, tubular, and normal proteinuria: determinations of urinary excretion of beta-2-microglobulin, albumin, and total protein. J Clin Invest 48:1189–1198PubMedPubMedCentralCrossRefGoogle Scholar
  25. 25.
    Pieper R, Gatlin CL, McGratz AM et al (2004) Characterization of the human urinary proteome: a method for high-resolution display of urinary proteins on two-dimensional electrophoresis gels with a yield of nearly 1400 distinct protein spots. Proteomics 4:1159–1174PubMedCrossRefGoogle Scholar
  26. 26.
    Poortmans JR (1984) Exercise and renal function. Sports Med 1:125–153PubMedCrossRefGoogle Scholar
  27. 27.
    Poortmans JR, Haralambie G (1979) Biochemical changes in a 100 km run: proteins in serum and urine. Eur J Appl Physiol Occup Physiol 40:245–254PubMedCrossRefGoogle Scholar
  28. 28.
    Poortmans JR, Jeanloz RW (1968) Quantitative immunological determination of 12 plasma proteins excreted in human urine collected before and after exercise. J Clin Invest 47:386–393PubMedPubMedCentralCrossRefGoogle Scholar
  29. 29.
    Poortmans JR, Vanderstraeten J (1994) Kidney function during exercise in healthy and diseased humans. Sports Med 18:419–437PubMedCrossRefGoogle Scholar
  30. 30.
    Poortmans JR, Labilloy D (1988) The influence of work intensity on postexercise proteinuria. Eur J Appl Physiol Occup Physiol 57:260–263PubMedCrossRefGoogle Scholar
  31. 31.
    Poortmans JR, Haggenmacher C, Vanderstraeten J (2001) Postexercise proteinuria in humans and its adrenergic component. J Sports Med Phys Fitness 41(1):95–100PubMedGoogle Scholar
  32. 32.
    Rennie D, Marticorena E, Monge C et al (1971) Urinary protein excretion in high-altitude residents. J Appl Phyisiol 3:259–275Google Scholar
  33. 33.
    Shavandi N, Samiei A, Afshar R et al (2012) The effect of exercise on urinary gamma-glutamyltransferase and protein levels in elite female karate athletes. Asian J Sports Med 3:41–46PubMedPubMedCentralGoogle Scholar
  34. 34.
    Thongboonkerd V, McLeish KR, Arthur JM et al (2002) Proteomic analysis of normal human urinary proteins isolated by acetone precipitation or ultracentrifugation. Kidney Int 62:1461–1469PubMedCrossRefGoogle Scholar
  35. 35.
    Williams H, Arnold H (1899) The effects of violent and prolonged muscular exercise upon the heart. Trans Am Climatol Assoc 15:267–285PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Maxie Kohler
    • 1
    • 2
  • Wilhelm Schänzer
    • 1
  • Mario Thevis
    • 1
    Email author
  1. 1.Institute of Biochemistry/Center for Preventive Doping ResearchGerman Sport University CologneCologneGermany
  2. 2.Chemistry DepartmentUniversity of CologneCologneGermany

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