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Principles and Practice of ABA Analysis

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Abscisic Acid: Metabolism, Transport and Signaling

Abstract

The development of fast, sensitive, and accurate analytical methods for abscisic acid (ABA) is of increasing importance for elucidation of its metabolism, transport, and molecular regulatory mechanisms in plants. In the past several decades, significant technical advances including sample preparation and detection techniques for ABA determination have been achieved. This chapter reviews the principles and practice in the extraction, purification, and analysis of ABA. Special attention is paid to the recent advances in the solid-phase extraction (SPE) and chromatographic methods coupled with mass spectrometry (MS), especially with tandem MS. A sum-up protocol based on analysis practice and service of several years in the authors’ laboratory is also presented.

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Acknowledgments

The work in the authors laboratory was financially supported by Natural Science Foundation of China (Grants 90817101, 91117006 and 91317312), Scientific Research Fund of Hunan Provincial Education Department (12K060, 12K061) and Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.

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  1. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Zhigang Huang, Mohammed Humayun Kabir, Yuan Xiao, Qing Liu, Jianhua Tong & Langtao Xiao

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  1. Zhigang Huang
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  2. Mohammed Humayun Kabir
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Correspondence to Langtao Xiao .

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  1. School of Life Sciences, Tsinghua University, Beijing, China

    Da-Peng Zhang

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Huang, Z., Kabir, M.H., Xiao, Y., Liu, Q., Tong, J., Xiao, L. (2014). Principles and Practice of ABA Analysis. In: Zhang, DP. (eds) Abscisic Acid: Metabolism, Transport and Signaling. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9424-4_21

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