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Production of Biomass and Bioactive Compounds from Cell Suspension Cultures of Panax quinquefolium L. and Glycyrrhiza uralensis Fisch.

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Production of Biomass and Bioactive Compounds Using Bioreactor Technology

Abstract

Panax quinquefolium L. and Glycyrrhiza uralensis Fisch. are important medicinal plants and health food that are used worldwide. Field cultivation of Panax quinquefolium and Glycyrrhiza uralensis is an extremely time consuming and labor-intensive process. Plant cell culture offers an alternative for obtaining valuable chemicals, especially plant-specific bioactive secondary metabolites. In this review, cell suspension cultures of Panax quinquefolium and Glycyrrhiza uralensis are described for the production of bioactive compounds.

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Abbreviations

2, 4-D:

2, 4-Dicholorphenoxy acetic acid

B5:

Gamborg’s medium

BA:

Benzyl adenine

BTBB:

Balloon-type bubble bioreactor

LH:

Lactalbumin hydrolysate

MJ:

Methyl jasmonate

MS:

Murashige and Skoog medium

NAA:

α-naphthalene acetic acid

PHE:

Phenylalanine

SOUR:

Specific oxygen uptake rate

vvm:

Air volume per culture volume per minute

References

  1. Qi LW, Wang CZ, Yuan CS (2011) Ginsenosides of American ginseng: chemical and pharmaceutical diversity. Phytochemistry 72:689–699

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  2. Christenson LP (2009) Ginsenosides chemistry, biosynthesis, analysis, and potential health effect. Adv Food Nutr Res 55:1–99

    Article  Google Scholar 

  3. Scholey A, Ossoukhova A, Owen L, Ibarra A, Pipingas A, He K, Roller M, Stough C (2010) Effect of American ginseng (Panax quiquefolius) on neurocongnitive function: an acute, randomized, double-blind, placeobo-controlled, crossover study. Psychopharmacology 201:345–356

    Article  Google Scholar 

  4. Zhang YH, Zhong JJ, Yu JT (1995) Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnol Lett 17:1347–1350

    CAS  Google Scholar 

  5. Nassiri M, Hosseinzadeh H (2008) Review on pharmacological effect of Glycyrrhiza sp. and its bioactive compounds. Phytother Res 22:709–724

    Article  Google Scholar 

  6. Williamson EM (2003) Liquorice. In: Daniels CW (ed) Potter’s cyclopedia of herbal medicines. C. W. Daniel, Saffron Walden, pp 269–271

    Google Scholar 

  7. Valeria MDM, Maria JVF (2005) Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts. J Pharm Biomed Anal 37:287–295

    Article  Google Scholar 

  8. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S (2003) Glabrene and isoliquiritigenin as tyrosinase inhibitors from Licorice roots. J Agric Food Chem 51:1201–1207

    Article  PubMed  CAS  Google Scholar 

  9. Wan FC, Cheng AW (2009) Polysaccharide isolated from Glycyrrhiza uralensis Fisch induces intracellular enzyme activity of macrophages. Mediterr J Nutr Metab 1:165–169

    Article  Google Scholar 

  10. Zhang HC, Liu JM, Chen HM, Gao CC, Lu HY, Zhou H, Li Y, Gao SL (2011) Up-regulation of licochalcone A biosynthesis and secretion by Tween 80 in hairy root cultures of Glycyrrhiza uralensis Fisch. Mol Biotechnol 47:50–56

    Article  PubMed  Google Scholar 

  11. Pengelly A, Bennett K (2011) Applachian plant monographs: Panax quinquefolius L., American ginseng. http://www.frostburg.efu/aces/appalachian-plants

  12. Gao WY, Jia W, Duan HQ, Xiao PG (2003) Industrialization of medicinal plant tissue culture. China J Chin Mat Med 28:385–390

    Google Scholar 

  13. Zhang XH, Zhao YF, Chen JM (2001) Determination of total flavonoids in Licorice. China Chin Mater Med 26:746–747

    CAS  Google Scholar 

  14. Zhang MP, Wang Y, Sun CY, Li XG (2003) Effects of different media and some element components on growth and saponin content of Panax quinquefolium Linn. by callus suspension culture. J Plant Resour Environ 12:14–16

    Google Scholar 

  15. Liu S, Zhong JJ (1998) Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium L. Process Biochem 33:69–74

    Article  CAS  Google Scholar 

  16. Zhang MP, Wang Y, Sun CY, Li XG (2003) Study on the consumption of sucrose and inorganic elements in the basal media of suspension culture of Panaxquin quefolium Linn. callus. J Plant Resour Environ 12:60–61

    CAS  Google Scholar 

  17. Zhang MP, Wang Y, Sun CY, Li XG (2003) Effect of matrixes pH and inoculation quantity to the Panax ginseng callus suspension culture. J Chin Med Mat 26:701

    Google Scholar 

  18. Zhang XB, Yan JH, Li YP, Wu M, Li CS, Chen YZ, Cheng H (2007) Studies on fermentation of suspension cells of Panax quinquefolium. Biotechnol Bull 5:188–193

    Google Scholar 

  19. Jeong JA, Wu CH, Murthy HN, Hahn EJ, Paek KY (2009) Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol Bioproc Eng 14:91–98

    Article  CAS  Google Scholar 

  20. Loc NH, Tuan VC, Binh DHN, Phuong TTB, Kim TG, Yang MS (2009) Accumulation of sesquiterpenes and polysaccharides in cells of Zedoary (Curcuma zedoaria Roscoe) cultured in a 10 L bioreactor. Biotechnol Bioproc Eng 14:619–624

    Article  Google Scholar 

  21. Dewir YH, Chakrabarty D, Wu CH, Hahn EJ, Jeon WK, Paek KY (2010) Influences of polyunsaturated fatty acids (PUFAs) on growth and secondary metabolite accumulation in Panax ginseng C.A. Meyer adventitious roots cultured in air-lift bioreactors. S Afr J Bot 76:354–358

    Article  CAS  Google Scholar 

  22. Sivakumar G, Yu KW, Paek KY (2005) Production of biomass and ginsenosides from adventitious roots of Panax ginseng in bioreactor cultures. Eng Life Sci 5:333–342

    Article  CAS  Google Scholar 

  23. Wang J, Gao WY, Zhang J, Huang T, Cao Y, Zhao YX (2010) Dynamic change of metabolites and nutrients in suspension cells of Panax quinquefolium L. in bioreactor. Acta Physiol Plant 32:463–467

    Article  Google Scholar 

  24. Schlatmann JE, Moreno PRH, Selles M, Vinke JL, Hoopen HJG, Verpoorte R, Heijnen JJ (1995) Two-stage batch process for the production of ajmalicine by Catharanthus roseus: the link between growth and production stage. Biotechnol Bioeng 47:53–59

    Article  PubMed  CAS  Google Scholar 

  25. Wang J, Gao WY, Zhang J, Huang T, Wen TT, Huang LQ (2011) Combination effect of lac albumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures. Acta Physiol Plant 33:861–866

    Article  CAS  Google Scholar 

  26. Mizukami H, Tabira Y, Ellis BE (1993) Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep 12:706–709

    Article  PubMed  CAS  Google Scholar 

  27. Wang J, Gao WY, Zhang J, Zuo BM, Zhang LM, Huang LQ (2012) Production of ginsenoside and polysaccharide by two-stage cultivation of Panax quinquefolium L. cells. In Vitro Cell Dev Biol Plant 48:107–112

    Article  Google Scholar 

  28. Yang Y, He F, Xiang J, Yu LJ (2007) Research on flavonoid in cell suspension culture of Glycyrrhiza. Hubei Agric Sci 46:680–683

    Google Scholar 

  29. Liu Y, Wei JF, Li DJ, Li JY (2006) Effects of rare earth elements on Glycyrrhiza cell growth and glycyrrhizic acid synthesis. Guihaia 26:101–104

    CAS  Google Scholar 

  30. Wang J, Zhang J, Gao WY, Wang Q, Yin SS, Liu H, Man SL (2013) Identification of triterpenoids and flavonoids, step-wise aeration treatment as well as antioxidant capacity of Glycyrrhiza uralensis Fisch. cell. Ind Crops Prod 49:675–681

    Article  CAS  Google Scholar 

  31. Meijer JJ, Hoopen HJG, Libbenga KR (1993) Effects of hydrodynamic stress on cultured plant cell: a literature survey. Enz Microb Technol 15:234–238

    Article  CAS  Google Scholar 

  32. Ahmed S, Hahn EJ, Paek KY (2008) Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures of Morinda citrifolia. J Plant Biol 51:209–212

    Article  CAS  Google Scholar 

  33. Zhong JJ, Yoshida M, Fujiyama K, Seki T, Yoshida T (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. J Ferment Bioengr 75:299–303

    Article  CAS  Google Scholar 

  34. Jeong CS, Debasis C, Hahn EJ, Lee HL, Paek KY (2006) Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263

    Article  CAS  Google Scholar 

  35. Lee EJ, Mobin M, Hahn EJ, Paek KY (2006) Effects of sucrose, inoculum density, auxins, and aeration volume on cell growth of Gymnema sylvestre. J Plant Biol 49:427–431

    Article  CAS  Google Scholar 

  36. Ryu DDY, Lee SO, Romani RJ (1990) Determination of growth rate for plant cell cultures: comparative studies. Biotechnol Bioeng 35:305–311

    Article  PubMed  CAS  Google Scholar 

  37. Georgiev MI, Weber J, Maciuk A (2009) Bioprocessing of plant cell cultures for mass production of targeted compounds. Appl Microbiol Biotechnol 83:809–823

    Article  PubMed  CAS  Google Scholar 

  38. Guo SB, Man SL, Gao WY, Liu H, Zhang LM, Xiao PG (2013) Production of flavonoids and polysaccharide by adding elicitor in different cellular cultivation processes of Glycyrrhiza uralensis Fisch. Acta Physiol Plant 35:679–686

    Article  CAS  Google Scholar 

  39. Yang Y, He F, Yu LJ (2008) Dynamics analyses of nutrients consumption and flavonoids accumulation in cell suspension culture of Glycyrrhiza inflate. Biol Plant 52:732–734

    Article  CAS  Google Scholar 

  40. Chen SY, Yang MZ, Hou GS (1994) Licorice cell culture by inner loop airlift bioreactor. J Wuhan Bot Res 12:380–384

    Google Scholar 

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Acknowledgments

This research was funded by Central Significant Increase or Decrease Program, China (20603020302), Tianjin University innovation fund, China (2013XQ-0046).

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Correspondence to Wen-Yuan Gao .

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Gao, WY., Wang, J., Li, J., Wang, Q. (2014). Production of Biomass and Bioactive Compounds from Cell Suspension Cultures of Panax quinquefolium L. and Glycyrrhiza uralensis Fisch.. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_7

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