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Production of Biomass and Bioactive Compounds Using Bioreactor Technology pp 251–283Cite as

Production of Adventitious Root Biomass and Bioactive Compounds from Hypericum perforatum L. Through Large Scale Bioreactor Cultures

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Abstract

Hypericum perforatum L. (St. John’s wort) is a traditional medicinal plant with antidepressive and woundhealing properties. It contains a lot of constituents with documented biological activity including phenolics, a broad range of flavonoids, naphthodianthrones and phloroglucinols. In recent years plant cell, tissue and organ cultures have been developed as an important alternative sources for the production of high value secondary metabolites. The adventitious roots of H. perforatum are regarded as an effective means of biomass production due to their fast growth rates and stable metabolite productivity. To determine optimal culture conditions for the bioreactor culture of H. perforatum adventitious roots, experiments have been conducted on various chemical and physical parameters in flasks and bioreactors. Adoption of elicitation methods have shown enhancement in the accumulation of total phenolics and flavonoids. Based on these results, a large scale bioreactor system at the industrial level (100 and 500 L) was established for the production of biomass and secondary metabolites from the adventitious root cultures. To investigate the usefulness of the adventitious root cultures for the production of secondary metabolites, comparison of ploidy level and the contents of total phenolics and flavonoids in adventitious roots and field-grown plant was conducted. In addition, identification of the major constituents in adventitious roots (hypericin, hyperin, quercetin and chlorogenic acid) were analyzed by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry (LC-MS/MS). Moreover, 12 isolated phenolic compounds from root cultures were evaluated for anti-inflammatory effects [Nuclear factor kappa B (NF-κB) inhibition and Peroxisome proliferator-activated receptor (PPAR) activation effects] and five xanthones of them were also tested for antioxidant and anticancer activities.

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Abbreviations

ABTS:

2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)

B5:

Gamborg

BA:

N6-benzyladenine

BTBB:

Balloon type bubble bioreactor

DCFDA:

2′,7′-dichlorfluorescein-diacetate

DPPH:

1, 1-diphenyl-2-picrylhydrazyl

DW:

Dry weight

EC:

Electrical conductivity

ESI:

Electrospray ionization

FW:

Fresh weight

H2O2 :

Hydrogen peroxide

HepG2:

Human hepatocarcinoma

IAA:

Indole-3-acetic acid

IBA:

Indole butyric acid

LC:

Liquid chromatography

LH:

Lactoalbumin hydrolysate

MDA:

Malondialdehyde

MJ:

Methyl jasmonate

MS:

Murashige and Skoog

MS/MS:

Tandem mass spectrometry

NAA:

1-naphthalene acetic acid

NF-κB:

Nuclear factor kappa B

PGR:

Plant growth regulator

PPAR:

Peroxisome proliferator-activated receptor

ROS:

Reactive oxygen species

SA:

Salicylic acid

TDZ:

Thidiazuron

TNF-α:

Tumor necrosis factor alpha

vvm:

Air volume · culture volume−1 · min−1

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Authors and Affiliations

  1. Biotechnology Research Institute, Yanbian Academy of Agriculture Sciences, Yanji, 133001, China

    Xi-Hua Cui

  2. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Hosakatte Niranjana Murthy & Kee-Yoeup Paek

  3. Department of Botany, Karnatak University, Dharwad, 580 003, India

    Hosakatte Niranjana Murthy & Kee-Yoeup Paek

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  1. Xi-Hua Cui
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  2. Hosakatte Niranjana Murthy
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  3. Kee-Yoeup Paek
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Correspondence to Xi-Hua Cui .

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Editors and Affiliations

  1. Department of Horticultural Science, Chungbuk National University, Cheongju, Korea, Republic of (South Korea)

    Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, India

    Hosakatte Niranjana Murthy

  3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

    Jian-Jiang Zhong

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Cui, XH., Murthy, H.N., Paek, KY. (2014). Production of Adventitious Root Biomass and Bioactive Compounds from Hypericum perforatum L. Through Large Scale Bioreactor Cultures. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_11

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