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Protein Microarrays: Overview, Applications and Challenges

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Genomics and Proteomics for Clinical Discovery and Development

Part of the book series: Translational Bioinformatics ((TRBIO,volume 6))

Abstract

Microarrays technology represents a new tool to address high-throughput biological studies. Various types of protein microarrays based on the application, format and content find use in basic research, drug and biomarker discovery, as well as for the characterization of proteins. In this chapter, we discuss advantages and limitations of protein microarrays, their features and recent applications. We also consider the different methods to build protein microarrays and the recent advances in cell free protein expression systems to construct in situ protein microarrays. Finally, we describe four types of self-assembled protein microarrays: PISA (protein array to protein Array); DAPA (DNA to Protein Array); PuCa (in situ puromycin array) and NAPPA (Nucleic Acids Programmable Protein Arrays) and the recent applications of this latter in situ protein array.

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Authors and Affiliations

  1. Instituto de Investigación BioMedica A Coruña, As Xubias de Arriba 84, 15006, A Coruña, Spain

    Lucia Lourido, Cristina Ruiz-Romero & Francisco Blanco

  2. Departamento de Medicina, Servicio General de Citometría, Centro de Investigación del Cáncer, IBSAL, Universidad de Salamanca-CSIC, Campus Miguel de Unamuno S/N, 37007, Salamanca, Spain

    Paula Diez, Noelia Dasilva, Maria Gonzalez-Gonzalez, Alberto Orfao & Manuel Fuentes Ph.D.

  3. Personalized Diagnostic Laboratory, Biodesign Institute, Arizona State University, Tempe, AZ, USA

    Joshua LaBaer MD, Ph.D.

  4. Proteomics Unit, Centro de Investigación del Cáncer, IBSAL, Universidad de Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain

    Manuel Fuentes Ph.D.

Authors
  1. Lucia Lourido
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  2. Paula Diez
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  3. Noelia Dasilva
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  4. Maria Gonzalez-Gonzalez
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  5. Cristina Ruiz-Romero
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  6. Francisco Blanco
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  7. Alberto Orfao
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  8. Joshua LaBaer MD, Ph.D.
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  9. Manuel Fuentes Ph.D.
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Corresponding authors

Correspondence to Joshua LaBaer MD, Ph.D. or Manuel Fuentes Ph.D. .

Editor information

Editors and Affiliations

  1. Clinical Protein Science & Imaging, Biomedical Center, Biomedical Engineering, Biomedical Center D13, Lund University, Lund, Sweden

    György Marko-Varga

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Lourido, L. et al. (2014). Protein Microarrays: Overview, Applications and Challenges. In: Marko-Varga, G. (eds) Genomics and Proteomics for Clinical Discovery and Development. Translational Bioinformatics, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9202-8_8

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