Abstract
The Human Proteome Project was recently launched with the aim of mapping all protein species of the Human Proteome and to develop resources for a better understanding of human biology in health and disease. The complexity of the proteome and its cell/tissue specific distribution has led the research community to the integration of transcriptomics and proteomics landscapes in a proteogenomics approach. This strategy opens new research avenues that will surely enhance our capacity to annotate those gene products and proteoforms that still lack of experimental evidences In this chapter a bioinformatics pipeline to integrate RNA-Seq data, shotgun proteomics experiments and tissue specific gene expression patterns is proposed.
Fernando J. Corrales and Victor Segura contributed equally to this work.
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Acknowledgments
All participating laboratories are members of ProteoRed-ISCIII. This work was supported by: ProteoRed and the Carlos III National Health Institute Agreement, ProteoRed-ISCIII; the agreement between FIMA and the “UTE project CIMA”; grants SAF2011-29312 from Ministerio de Ciencia e Innovación and ISCIII-RETIC RD06/0020 to FJC. We also thank the Proteomics, Genomics and Bioinformatics Core Facility of CIMA.
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Guruceaga, E., Monteiro, M.B., Mora, M.I., Ortiz, L., Corrales, F.J., Segura, V. (2014). Chromosome Transcriptome Profiling in the Context of High-Throughput Proteomics Studies. In: Marko-Varga, G. (eds) Genomics and Proteomics for Clinical Discovery and Development. Translational Bioinformatics, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9202-8_3
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