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Validation of Candidate Genes Expression Profiles During Cold Acclimation and Freezing in Perennial Ryegrass

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Quantitative Traits Breeding for Multifunctional Grasslands and Turf

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Abstract

Sixty perennial ryegrass (Lolium perenne L) plants were cold-acclimated and then subjected to freezing at −10 °C. Quantitative real-time polymerase chain reaction was performed to analyse the expression of six candidate genes during cold-acclimation and freezing periods. Four genes responded similarly in two investigated genotypes during both hardening and freezing periods, while the cold-response genes LpIRI and LOS2 distinguished high induction level. These genes represent valuable candidates for further SNP analysis to identify functional markers for cold tolerance in perennial ryegrass.

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Acknowledgement

Postdoctoral fellowship is being funded by European Union Structural Funds project “Postdoctoral Fellowship Implementation in Lithuania” No. VP1-3.1-ŠMM-01-V-02-004/36

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Authors and Affiliations

  1. Laboratory of Genetics and Physiology, Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Instituto av. 1, Akademija, Kedainiai, LT-58344, Lithuania

    Kristina Jonavičienė & Gintaras Brazauskas

  2. Department of Agroecology, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark

    Shamila Abeynayake

  3. Department of Molecular Biology and Genetics, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark

    Torben Asp

Authors
  1. Kristina Jonavičienė
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  2. Shamila Abeynayake
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  3. Gintaras Brazauskas
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  4. Torben Asp
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Corresponding author

Correspondence to Kristina Jonavičienė .

Editor information

Editors and Affiliations

  1. Institute for Forage Crops, Kruševac, Serbia

    Dejan Sokolović

  2. SDAR, INRA, Lusignan, France

    Christian Huyghe

  3. Institute for Forage Crops, Kruševac, Serbia

    Jasmina Radović

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© 2014 Springer Science+Business Media Dordrecht

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Jonavičienė, K., Abeynayake, S., Brazauskas, G., Asp, T. (2014). Validation of Candidate Genes Expression Profiles During Cold Acclimation and Freezing in Perennial Ryegrass. In: Sokolović, D., Huyghe, C., Radović, J. (eds) Quantitative Traits Breeding for Multifunctional Grasslands and Turf. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9044-4_38

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