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Use of Airborne Inoculum Detection for Disease Management Decisions

Chapter
Part of the Plant Pathology in the 21st Century book series (ICPP, volume 5)

Abstract

Knowledge of inoculum presence has been used for decades to help guide disease management decisions. However, its implementation on a broad scale has been limited due to the capital costs and technical skill required to effectively monitor pathogen presence across large areas. Recent advances in nucleic acid detection technologies are showing promise in enabling field level implementation of inoculum detection and quantification to aid in disease management decisions. Over the past 7 years we have investigated the use various molecular approaches to monitor the presence of Erysiphe necator in commercial vineyards and time fungicide applications based on its detection. We have found loop mediated isothermal amplification (LAMP) to be a robust method for the detection of E. necator DNA that may be suitable for practitioner implementation.

Keywords

LAMP qPCR Inoculum detection Airbiotia Spore dispersion IPM 

Notes

Acknowledgments

This work was supported by USDA-ARS CRIS 5358-22000-039-OOD, American Vineyard Foundation, Oregon Wine Board, and Viticulture Consortium -West. I thank Tyrone Hall, David Gent, Gary Grove, Ken Johnson, Jessica Keune, Tara Neill, Jay Pscheidt, Todd Temple and Lindsey Thiessen for discussions that guided the ideas presented above. I also thank numerous practitioners that have helped refine the E. necator inoculum detection project and help bring it to commercialization. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.United States Department of Agriculture – Agricultural Research ServiceHorticultural Crops Research UnitCorvallisUSA
  2. 2.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA

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