Abstract
This perspective presents the possibilities for utilization of gold nanoparticles in immunocytochemistry. The unique combination of gold nanoparticle properties is just beginning to be fully recognized for the range of diagnostic and therapeutic applications. The current findings from bionanotechnology and emerging cryo-electron microscopy techniques can lead to novel perspectives for targeted delivery of gold conjugates into cells and tissues. The chapter offers a survey of modern electron microscopy methods with a special focus on immunolabeling. We also discuss the impact of gold nanoparticles on cellular systems and the potential possibilities for their targeted delivery into cells and tissues.
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Abbreviations
- CEMOVIS:
-
Cryo-electron microscopy of the vitrified sections
- CLEM:
-
Correlation light-electron microscopy
- EM:
-
Electron microscopy
- FM:
-
Fluorescence microscopy
- FS:
-
Freeze substitution
- GNP:
-
Gold nanoparticle
- HPF:
-
High pressure freezing
- IEM:
-
Immuno-electron microscopy
- LM:
-
Light microscopy
- NP:
-
Nanoparticle
- RME:
-
Receptor mediated endocytosis
- ROS:
-
Reactive oxygen species
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Acknowledgements
This book chapter was supported by the Grant Agency of the Czech Republic (grant number P302/12/G157) and by the Charles University in Prague (projects UNCE 204022 and Prvouk/1LF/1). This publication is also supported by the project “BIOCEV—Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University” (CZ.1.05/1.1.00/02.0109), from the European Regional Development Fund and project CZ.2.16/3.1.00/24010 from the European Regional Development Fund. Many thanks to Guy Hagen for the language corrections.
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Schröfel, A., Cmarko, D., Bártová, E., Raška, I. (2014). Gold Nanoparticles for High Resolution Imaging in Modern Immunocytochemistry. In: Prokop, A., Iwasaki, Y., Harada, A. (eds) Intracellular Delivery II. Fundamental Biomedical Technologies, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8896-0_9
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