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Intracellular Delivery of RNA via RNA-Binding Proteins or Peptides

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Intracellular Delivery II

Part of the book series: Fundamental Biomedical Technologies ((FBMT,volume 7))

Abstract

Methods that facilitate the cellular internalization of RNAs are widely utilized in medical and biological studies. Numerous small RNA carriers have been reported, many of which are comprised of synthetic polymers. Nanocarriers based on other materials, such as silica and metal nanoparticles, have also been developed. In this chapter, we describe methods for the intracellular delivery of small RNAs using carrier peptides, primarily cationic cell-penetrating peptides (CPPs) and carrier proteins, primarily CPP-RNA binding domain fusion proteins. In addition, we discuss the advantages and drawbacks of these carriers compared to other carriers.

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Abbreviations

siRNA:

Small interfering RNA

miRNA:

Micro RNA

dsRNA:

Double-stranded RNA

RBP:

RNA-binding protein

RBD:

RNA-binding domain

DRBD:

DsRNA-binding domain

CPP:

Cell-penetrating peptide

PTD:

Peptide transduction domain

NLS:

Nuclear localization signal

CLIP-RNAi:

CPP-linked RBP-mediated RNA internalization and photoinduced RNAi

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Acknowledgements

We thank S. Matsumoto for help with preparation of Fig. 1.

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Authors and Affiliations

  1. Department of Biotechnology, Okayama University, 3-1-1 Tsushima-naka, Kita-ku, Okayama, 700-8530, Japan

    Kazunori Watanabe & Takashi Ohtsuki

Authors
  1. Kazunori Watanabe
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  2. Takashi Ohtsuki
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Correspondence to Takashi Ohtsuki .

Editor information

Editors and Affiliations

  1. Chemical and Biological Engineering, Vanderbilt University, Nashville, Tennessee, USA

    Aleš Prokop

  2. Chemistry and Materials Engineering, Kansai University, Osaka, Japan

    Yasuhiko Iwasaki

  3. Department of Applied Chemistry, Osaka Prefecture University, Osaka, Japan

    Atsushi Harada

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Watanabe, K., Ohtsuki, T. (2014). Intracellular Delivery of RNA via RNA-Binding Proteins or Peptides. In: Prokop, A., Iwasaki, Y., Harada, A. (eds) Intracellular Delivery II. Fundamental Biomedical Technologies, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8896-0_19

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