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Bioelectronic Nose pp 145–170Cite as

Production of Olfactory Receptors and Nanovesicles Using Heterologous Cell Systems for Bioelectronic Nose

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Abstract

Olfactory receptors, belonging to a family of G protein-coupled receptors (GPCRs), which are involved in various important physiological processes, are integral membrane proteins composed of seven transmembrane helices. It is difficult to produce GPCRs including olfactory receptors (ORs) using heterologous cell systems, because of their strong hydrophobicity, and complicated structure. The production of ORs should be a critical process for the development of an olfactory receptor-based bioelectronic nose. Significant efforts have been made for the production of ORs for the utilization as recognition elements of bioelectronic noses, and also for other applications. In addition, the construction method of mammalian cell-derived nanovesicles containing ORs has been demonstrated and applied for an bioelectronic nose, due to their unique properties, and suitable size for integration with a nanosensor platform. In this chapter, advances in the production of ORs and nanovesicles using various heterologous cell systems for the development of a bioelectronic nose are described.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning (No. 2013003890, No. 2013055375).

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  1. School of Chemical and Biological Engineering, Seoul National University, 151-744, Seoul, Republic of Korea

    Hyun Seok Song & Tai Hyun Park

  2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    Hyun Seok Song

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Correspondence to Tai Hyun Park .

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    Tai Hyun Park

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Song, H., Park, T. (2014). Production of Olfactory Receptors and Nanovesicles Using Heterologous Cell Systems for Bioelectronic Nose. In: Park, T. (eds) Bioelectronic Nose. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8613-3_9

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