Abstract
Both the sensillary lymph of insects and the nasal mucus of vertebrates contain large amounts of small soluble proteins, odorant-binding proteins that specifically and reversibly bind odors and pheromones. Proteins from different sources have affinities toward a wide range of compounds with different sizes and shapes. They can be easily expressed in heterologous systems, they show high thermal stability and it is possible to modify their binding sites by site-directed mutagenesis. We describe the development of an odor sensing biosensor array based on immobilization of odorant binding proteins on to suitable transducers. Using a quartz crystal microbalance platform as a transduction element, it is possible to detect and measure quantitatively concentrations of volatile analytes at parts per million concentrations in air.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Turner AP (2013) Biosensors: sense and sensibility. Chem Soc Rev 42(8):3184–3196
Arora P, Sindhu A, Kaur H, Dilbaghi N, Chaudhury A (2013) An overview of transducers as platform for the rapid detection of foodborne pathogens. Appl Microbiol Biotechnol 97(5):1829–1840
Buck LB (2004) Olfactory receptors and odor coding in mammals. Nutr Rev 62(11):S184–S188
Persaud KC (2012) Biomimetic olfactory sensors. Ieee Sens J 12(11):3108–3112
Du L, Wu C, Liu Q, Huang L, Wang P (2013) Recent advances in olfactory receptor-based biosensors. Biosens Bioelectron 42:570–580
Pelosi P (2001) The role of perireceptor events in vertebrate olfaction. Cell Mol Life Sci 58(4):503–509
Persaud KC, Ng SM, Mucignat C, Pelosi P (2009) Odorant binding proteins and mouse urinary proteins: potential biomimetic sensing systems. Chem Senses 34(3):E36–E37
Lucarelli F, Tombelli S, Minunni M, Marrazza G, Mascini M (2008) Electrochemical and piezoelectric DNA biosensors for hybridisation detection. Anal Chim Acta 609(2):139–159
Mascini M, Tombelli S (2008) Biosensors for biomarkers in medical diagnostics. Biomarkers 13(7–8):637–657
De Corcuera JIR, Cavalieri RP (2003) Biosensors. Encyclopedia of agricultural, food, and biological engineering. Ed. Dennis Heldman, Pub. Marcel-Dekker, New York (ISBN 0-8247-0938-1) pp 119–123
Erden PE, Kilic E (2013) A review of enzymatic uric acid biosensors based on amperometric detection. Talanta 107:312–323
Labuda J, Oliveira Brett AM, Evtugyn G, Fojta M, Mascini M, Ozsoz M et al (2010) Electrochemical nucleic acid-based biosensors: concepts, terms, and methodology (IUPAC technical report). Pure Appl Chem 82(5):1161–1187
Liu Y, Du Y, Li CM (2013) Direct electrochemistry based biosensors and biofuel cells enabled with nanostructured materials. Electroanalysis 25(4):815–831
Daniels JS, Pourmand N (2007) Label-free impedance biosensors: opportunities and challenges. Electroanalysis 19(12):1239–1257
Wang XD, Wolfbeis OS (2013) Fiber-Optic chemical sensors and biosensors (2008–2012). Anal Chem 85(2):487–508
Manera M, Spadavecchia J, Leone A, Quaranta F, Rella R, Dell’atti D et al (2008) Surface plasmon resonance imaging technique for nucleic acid detection. Sens Actuators B-Chem 130(1):82–87
Scarano S, Scuffi C, Mascini M, Minunni M (2011) Surface plasmon resonance imaging-based sensing for anti-bovine immunoglobulins detection in human milk and serum. Anal Chim Acta 707(1):178–183
Scarano S, Mascini M, Turner AP, Minunni M (2010) Surface plasmon resonance imaging for affinity-based biosensors. Biosens Bioelectron 25(5):957–966
Glaser RW (2000) Surface plasmon resonance biosensors. In: Yang VCM, Ngo TT (eds) Biosensors and their applications. Kluwer Academic/Plenum Publishers, pp 195–212
Lec RM (2001) Piezoelectric biosensors: recent advances and applications. Proceedings of the 2001 Ieee International Frequency Control Symposium & Pda Exhibition, pp 419–429
Ferreira GN, da-Silva AC, Tome B (2009) Acoustic wave biosensors: physical models and biological applications of quartz crystal microbalance. Trends Biotechnol 27(12):689–697
Speight RE, Cooper MA (2012) A survey of the 2010 quartz crystal microbalance literature. J Mol Recognit 25(9):451–473
Sauerbrey G (1959) Verwendung Von Schwingquarzen Zur Wagung Dunner Schichten und Zur Mikrowagung. Zeitschrift fur Physik 155(2):206–222
Putzbach W, Ronkainen NJ (2013) Immobilization techniques in the fabrication of nanomaterial-based electrochemical biosensors: a review. Sensors 13(4):4811–4840
Senveli SU, Tigli O (2013) Biosensors in the small scale: methods and technology trends. Iet Nanobiotechnol 7(1):7–21
Wang R, Zhang Y, Lu D, Ge J, Liu Z, Zare RN (2013) Functional protein-organic/inorganic hybrid nanomaterials. Wiley Interdiscip Rev Nanomed Nanobiotechnol 5(4):320–328
Caruso F, Rinia HA, Furlong DN (1996) Gravimetric monitoring of nonionic surfactant adsorption from nonaqueous media onto quartz crystal microbalance electrodes and colloidal silica. Langmuir 12(9):2145–2152
Lin JN, Drake B, Lea AS, Hansma PK, Andrade JD (1990) Direct observation of immunoglobulin adsorption dynamics using the atomic force microscope. Langmuir 6(2):509–511
Giuseppone N (2012) Toward self-constructing materials: a systems chemistry approach. Acc Chem Res 45(12):2178–2188
Koepsel JT, Murphy WL (2012) Patterned self-assembled monolayers: efficient, chemically defined tools for cell biology. Chembiochem 13(12):1717–1724
Deshmukh PK, Ramani KP, Singh SS, Tekade AR, Chatap VK, Patil GB, et al (2013) Stimuli-sensitive layer-by-layer (LbL) self-assembly systems: targeting and biosensory applications. J Control Release 166(3):294–306
Ulman A (1996) Formation and structure of self-assembled monolayers. Chem Rev 96(4):1533–1554
Wink T, vanZuilen SJ, Bult A, vanBennekom WP (1997) Self-assembled monolayers for biosensors. Analyst 122(4):R43–R50
Kajiya Y, Sugai H, Iwakura C, Yoneyama H (1991) Glucose sensitivity of polypyrrole films containing immobilized glucose-oxidase and hydroquinonesulfonate ions. Anal Chem 63(1):49–54
Collings AF, Caruso F (1997) Biosensors: recent advances. Rep Prog Phys 60(11):1397–1445
Miao Y, Chia LS, Goh NK, Tan SN (2001) Amperometric glucose biosensor based on immobilization of glucose oxidase in chitosan matrix cross-linked with glutaraldehyde. Electroanalysis 13(4):347–349
Pelosi P, Baldaccini NE, Pisanelli AM (1982) Identification of a specific olfactory receptor for 2-isobutyl-3-methoxypyrazine. Biochem J 201(1):245–248
Vogt RG, Riddiford LM (1981) Pheromone binding and inactivation by moth antennae. Nature 293(5828):161–163
Pelosi P, Calvello M, Ban LP (2005) Diversity of odorant-binding proteins and chemosensory proteins in insects. Chem Senses 30:i291–i292
Pelosi P, Zhou J, Ban L, Calvello M (2006) Soluble proteins in insect chemical communication. Cell Mol Life Sci 63(14):1658–1676
Leal WS (2013) Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annu Rev Entomol 58:373–391
Cavaggioni A, Mucignat-Caretta C (2000) Major urinary proteins, alpha(2u)-globulins and aphrodisin. Biochim Biophys Acta-Protein Struct Mol Enzymol 1482(1–2):218–228
Marchlewska-Koj A, Cavaggioni A, Mucignat-Caretta C, Olejniczak P (2000) Stimulation of estrus in female mice by male urinary proteins. J Chem Ecol 26(10):2355–2366
Hurst JL, Payne CE, Nevison CM, Marie AD, Humphries RE, Robertson DHL, et al (2001) Individual recognition in mice mediated by major urinary proteins. Nature 414(6864):631–634
Cavaggioni A, Mucignat C, Tirindelli R (1999) Pheromone signalling in the mouse: role of urinary proteins and vomeronasal organ. Arch Ital Biol 137(2–3):193–200
Mucignat-Caretta C, Cavaggioni A, Caretta A (2004) Male urinary chemosignals differentially affect aggressive behavior in male mice. J Chem Ecol 30(4):777–791
Marchese S, Pes D, Scaloni A, Carbone V, Pelosi P (1998) Lipocalins of boar salivary glands binding odours and pheromones. Eur J Biochem 252(3):563–568
Loebel D, Scaloni A, Paolini S, Fini C, Ferrara L, Breer H et al (2000) Cloning, post-translational modifications, heterologous expression and ligand-binding of boar salivary lipocalin. Biochem J 350:369–379
Pevsner J, Hou V, Snowman AM, Snyder SH (1990) Odorant-binding protein—characterization of ligand-binding. J Biol Chem 265(11):6118–6125
Pevsner J, Snyder SH (1990) Odorant-binding protein—odorant transport function in the vertebrate nasal epithelium. Chem Senses 15(2):217–222
Vogt RG (1991) Pheromone and general-odorant binding-proteins in Lepidoptera. Am Zool 31(5):A16
Vogt RG (1992) Multiple classes of insect OBP provide a functional and developmental model for olfactory specificity. Chem Senses 17(5):712–713
Wang Q, Hasan G, Pikielny CW (1999) Preferential expression of biotransformation enzymes in the olfactory organs of Drosophila melanogaster, the antennae. J Biol Chem 274(15):10309–10315
Lobel D, Marchese S, Krieger J, Pelosi P, Breer H (1998) Subtypes of odorant-binding proteins—heterologous expression and ligand binding. Eur J Biochem 254(2):318–324
Lobel D, Jacob M, Volkner M, Breer H (2002) Odorants of different chemical classes interact with distinct odorant binding protein subtypes. Chem Senses 27(1):39–44
Kim MS, Repp A, Smith DP (1998) LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster. Genetics 150(2):711–721
Xu PX, Atkinson R, Jones DNM, Smith DP (2005) Drosophila OBP LUSH is required for activity of pheromone-sensitive neurons. Neuron 45(2):193–200
Xu PX (2005) Eppendorf 2005 winner—a Drosophila OBP required for pheromone signaling. Science 310(5749):798–799
Vogt RG, Kohne AC, Dubnau JT, Prestwich GD (1989) Expression of pheromone binding-proteins during antennal development in the gypsy-moth Lymantria dispar. J Neurosci 9(9):3332–3346
Gyorgyi TK, Robyshemkovitz AJ, Lerner MR (1988) Characterization and cDNA cloning of the pheromone-binding protein from the tobacco hornworm, Manduca sexta—a tissue-specific developmentally regulated protein. Proc Natl Acad Sci U S A 85(24):9851–9855
Krieger J, von Nickisch-Rosenegk E, Mameli M, Pelosi P, Breer H (1996) Binding proteins from the antennae of Bombyx mori. Insect Biochem Mol Biol 26(3):297–307
Hekmat-Scafe DS, Scafe CR, McKinney AJ, Tanouye MA (2002) Genome-wide analysis of the odorant-binding protein gene family in Drosophila melanogaster. Genome Res 12(9):1357–1369
Riviere S, Lartigue A, Quennedey B, Campanacci V, Farine JP, Tegoni M et al (2003) A pheromone-binding protein from the cockroach Leucophaea maderae: cloning, expression and pheromone binding. Biochem J 371:573–579
Vieira FG, Sanchez-Gracia A, Rozas J (2007) Comparative genomic analysis of the odorant-binding protein family in 12 Drosophila genomes: purifying selection and birth-and-death evolution. Genome Biol 8(11):R235
Xu PX, Zwiebel LJ, Smith DP (2003) Identification of a distinct family of genes encoding atypical odorant-binding proteins in the malaria vector mosquito, Anopheles gambiae. Insect Mol Biol 12(6):549–560
Zhou JJ, Zhang GA, Huang WS, Birkett MA, Field LM, Pickett JA et al (2004) Revisiting the odorant-binding protein LUSH of Drosophila melanogaster: evidence for odour recognition and discrimination. Febs Lett 558(1–3):23–26
Zhou JJ, Huang WS, Zhang GA, Pickett JA, Field LM (2004) “Plus-C” odorant-binding protein genes in two Drosophila species and the malaria mosquito Anopheles gambiae. Gene 327(1):117–129
Vieira FG, Rozas J (2011) Comparative genomics of the odorant-binding and chemosensory protein gene families across the arthropoda: origin and evolutionary history of the chemosensory system. Genome Biol Evol 3:476–490
Dalmonte M, Andreini I, Revoltella R, Pelosi P (1991) Purification and characterization of 2 odorant-binding proteins from nasal tissue of rabbit and pig. Comp Biochem Physiol B-Biochem Mol Biol 99(2):445–451
Pes D, Dalmonte M, Ganni M, Pelosi P (1992) Isolation of 2 odorant-binding proteins from mouse nasal tissue. Comp Biochem Physiol B-Biochem Mol Biol 103(4):1011–1017
Felicioli A, Ganni M, Garibotti M, Pelosi P (1993) Multiple types and forms of odorant-binding proteins in the old-world porcupine Hystrix cristata. Comp Biochem Physiol B-Biochem Mol Biol 105(3–4):775–784
Lacazette E, Gachon AM, Pitiot G (2000) A novel human odorant-binding protein gene family resulting from genomic duplicons at 9q34: differential expression in the oral and genital spheres. Hum Mol Genet 9(2):289–301
Hwang PM, Pevsner J, Sklar PB, Venable JC, Snyder SH (1988) Localization of rat odorant-binding protein to the lateral nasal gland suggests an odorant transport function. Chem Senses 13(4):699
Pevsner J, Snyder SH (1990) Odorant-binding protein—odorant transport function in the vertebrate nasal epithelium. Chem Senses 15(2):217–222
Utsumi M, Ohno K, Kawasaki Y, Tamura M, Kubo T, Tohyama M (1999) Expression of major urinary protein genes in the nasal glands associated with general olfaction. J Neurobiol 39(2):227–236
Flower DR (1996) The lipocalin protein family: structure and function. Biochem J 318:1–14
Flower DR, North ACT, Sansom CE (2000) The lipocalin protein family: structural and sequence overview. Biochim Biophys Acta-Protein Struct Mol Enzymol 1482(1–2):9–24
Napolitano E, Pelosi P (1992) Synthesis of thiazole and selenazole derivatives with affinity for the odorant-binding protein. Bioorg Med Chem Lett 2(12):1603–1606
Pelosi P, Dal MM (1990) Purification and characterization of odorant binding proteins from nasal mucosa of pig and rabbit. Chem Senses 15(5):614
Dalmonte M, Centini M, Anselmi C, Pelosi P (1993) Binding of selected odorants to bovine and porcine odorant-binding proteins. Chem Senses 18(6):713–721
Mecea VM (2005) From quartz crystal microbalance to fundamental principles of mass measurements. Anal Lett 38(5):753–767
Acknowledgements
Elena Tuccori was supported via a Marie Curie Early Stage Researcher grant (FLEXSMELL GA-2009-238454).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Persaud, K., Tuccori, E. (2014). Biosensors Based on Odorant Binding Proteins. In: Park, T. (eds) Bioelectronic Nose. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8613-3_10
Download citation
DOI: https://doi.org/10.1007/978-94-017-8613-3_10
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-8612-6
Online ISBN: 978-94-017-8613-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)