Skip to main content
SpringerLink
Log in

Combining Single Crystal UV/Vis Spectroscopy and Diffraction to Structurally Characterise Intermediates and Monitor Radiation Damage

  • Conference paper
  • First Online:
The Future of Dynamic Structural Science

  • 741 Accesses

Abstract

Mechanisms in biology are dynamic processes and changes in macromolecular structures are intimately linked to their function. Without insight into the dynamic workings of these molecules, we cannot fully understand them. In an ideal world, a complete time-resolved description of each stage of the reaction pathway, at high resolution, would be obtained. Is this possible in practice?

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Mozzarelli A, Rossi G-L (1996) Annu Rev Biophys Biomol Struct 25:343–365

    Article  CAS  Google Scholar 

  2. Makinen MW, Fink AL (1977) Annu Rev Biophys Bioeng 6:301–343

    Article  CAS  Google Scholar 

  3. Wilmot CM, Sjögren T, Carlsson GH, Berglund GI, Hajdu J (2002) Methods Enzymol 353:301–318

    Article  CAS  Google Scholar 

  4. Pearson AR, Owen RL (2009) Biochem Soc Trans 37:378–381

    Article  CAS  Google Scholar 

  5. Sjögren T, Carlsson G, Larsson G, Hajdu A, Andersson C, Pettersson H, Hajdu J (2002) J Appl Cryst 35:113–116

    Article  Google Scholar 

  6. Merli A, Brodersen DE, Morini B, Chen Z, Durley RC, Mathews FS, Davidson VL, Rossi GL (1996) J Biol Chem 271:9177–9180

    Article  CAS  Google Scholar 

  7. Carey PR (2006) Ann Rev Phys Chem 57:527–554

    Article  CAS  Google Scholar 

  8. Pearson AR, Wilmot CM (2003) Biochimica et biophysica acta 1647:381–389

    Article  CAS  Google Scholar 

  9. Kovaleva EG, Lipscomb JD (2008) Biochemistry 47:11168–11170

    Article  CAS  Google Scholar 

  10. Wilmot CM, Hajdu J, McPherson MJ, Knowles PF, Phillips SEV (1999) Science 286:1724–1728

    Article  CAS  Google Scholar 

  11. Kovaleva EG, Lipscomb JD (2007) Science 316:453–457

    Article  CAS  Google Scholar 

  12. Sjögren T, Hajdu J (2001) J Biol Chem 276:13072–13076

    Article  Google Scholar 

  13. Douzou P (1977) Cryobiochemistry: an introduction. Academic, London/New York

    Google Scholar 

  14. Garman EF (2010) Acta Cryst D66:339–351

    Google Scholar 

  15. Orville AM, Buono R, Cowan M, Heroux A, Shea-McCarthy G, Schneider DK, Skinner JM, Skinner MJ, Stoner-Ma D, Sweet RM (2011) J Synch Rad 18:358–366

    Article  CAS  Google Scholar 

  16. Pearson AR, Mozzarelli A (2011) Biochim Biophys Acta 1814:731–733

    Article  CAS  Google Scholar 

  17. Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG (2000) Science 287:1615–1622

    Article  CAS  Google Scholar 

  18. Berglund GI, Carlsson GH, Smith AT, Szöke H, Henriksen A, Hajdu J (2002) Nature 417:463–468

    Article  CAS  Google Scholar 

  19. Pollack L, Tate MW, Darnton NC, Knight JB, Gruner SM, Eaton WA, Austin RH (1999) Proc Natl Acad Sci U S A 96:10115–10117

    Article  CAS  Google Scholar 

  20. Pollack L, Tate MW, Finnefrock AC, Kalidas C, Trotter S, Darnton NC, Lurio L, Austin RH, Batt CA, Gruner SM, Mochrie SG (2001) Phys Rev Lett 86:4962–4965

    Article  CAS  Google Scholar 

  21. Lamb JS, Zoltowski BD, Pabit SA, Crane BR, Pollack L (2008) J Am Chem Soc 130:12226–12227

    Article  CAS  Google Scholar 

  22. Schmitz A, Galas DJ (1980) Nucleic Acids Res 8:487–506

    Article  CAS  Google Scholar 

  23. Ward JF (1988) Prog Nucleic Acid Res Mol Biol 35:95–125

    Article  CAS  Google Scholar 

  24. Chance MR, Sclavi B, Woodson SA, Brenowitz M (1997) Structure 5:865–869

    Article  CAS  Google Scholar 

  25. Xu G, Takamoto K, Chance MR (2003) Anal Chem 75:6995–7007

    Article  CAS  Google Scholar 

  26. Gupta S, Bavro VN, D'Mello R, Tucker SJ, Venien-Bryan C, Chance MR (2010) Structure 18:839–846

    Article  CAS  Google Scholar 

  27. Raschke TM, Marqusee S (1998) Curr Opin Biotech 9:80–86

    Article  CAS  Google Scholar 

  28. Knapman TW, Morton VL, Stonehouse NJ, Stockley PG, Ashcroft AE (2010) Rapid Commun Mass Spec 24:3033–3042

    Article  CAS  Google Scholar 

  29. Schuler B, Eaton WA (2008) Curr Opin Struct Biol 18:16–26

    Article  CAS  Google Scholar 

  30. Otting G (2010) Annu Rev Biophys 39:387–405

    Article  CAS  Google Scholar 

  31. Van Wonderen J, Kostrz DN, Dennison C, MacMillan F (2013) Angew Chem Int Ed 52:1990–1993

    Article  Google Scholar 

  32. White HD, Thirumurugan K, Walker ML, Trinick J (2003) J Struct Biol 144:246–252

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Astbury Centre for Structural Molecular Biology, The University of Leeds, Leeds, UK

    Anna Polyakova & Arwen R. Pearson

Authors
  1. Anna Polyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arwen R. Pearson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arwen R. Pearson .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Durham University, Durham, United Kingdom

    Judith A.K. Howard

  2. School of Chemistry, University of Bristol, Bristol, United Kingdom

    Hazel A. Sparkes

  3. Department of Chemistry, University of Bath, United Kingdom

    Paul R. Raithby

  4. N.S. Kurnakov Institute of General and Inorganic Chemistry Russian Academy of Sciences, Moscow, Russia

    Andrei V. Churakov

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Polyakova, A., Pearson, A.R. (2014). Combining Single Crystal UV/Vis Spectroscopy and Diffraction to Structurally Characterise Intermediates and Monitor Radiation Damage. In: Howard, J., Sparkes, H., Raithby, P., Churakov, A. (eds) The Future of Dynamic Structural Science. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8550-1_18

Download citation

Publish with us

Policies and ethics

Search

Navigation