Smell Sensors Based on Olfactory Receptor



The discovery of olfactory receptors (ORs) multigene superfamily promotes the research in the field of olfactory molecular mechanisms and signal transduction pathways greatly.


Olfactory Receptor Olfactory Epithelium Olfactory System Olfactory Sensory Neuron Main Olfactory Bulb 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Buck L, Axel R. A novel multigene family may encode odorant receptors—a molecular-basis for odor recognition. Cell. 1991;65(1):175–87.CrossRefPubMedGoogle Scholar
  2. 2.
    Breer H. Olfactory receptors: molecular basis for recognition and discrimination of odors. Anal Bioanal Chem. 2003;377(3):427–33.CrossRefPubMedGoogle Scholar
  3. 3.
    Firestein S, Zhao HQ, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K. Functional expression of a mammalian odorant receptor. Science. 1998;279(5348):237–42.CrossRefPubMedGoogle Scholar
  4. 4.
    Glatz R, Bailey-Hill K. Mimicking nature’s noses: from receptor deorphaning to olfactory biosensing. Prog Neurobiol. 2011;93(2):270–96.CrossRefPubMedGoogle Scholar
  5. 5.
    Touhara K. Deorphanizing vertebrate olfactory receptors: recent advances in odorant-response assays. Neurochem Int. 2007;51(2–4):132–9.CrossRefPubMedGoogle Scholar
  6. 6.
    Pevsner J, Reed RR, Feinstein PG, Snyder SH. Molecular-cloning of odorant-binding protein—member of a ligand carrier family. Science. 1988;241(4863):336–9.CrossRefPubMedGoogle Scholar
  7. 7.
    Beccherelli R, Zampetti E, Pantalei S, Bernabei M, Persaud KC. Design of a very large chemical sensor system for mimicking biological olfaction. Sens Actuators B Chem 2010;146(2):446–452.Google Scholar
  8. 8.
    Muezzinoglu MK, Huerta R, Abarbanel HDI, Ryan MA, Rabinovich MI. Chemosensor-driven artificial antennal lobe transient dynamics enable fast recognition and working memory. Neural Comput. 2009;21(4):1018–37.CrossRefPubMedGoogle Scholar
  9. 9.
    Göpel W, Ziegler C, Breer H, Schild D, Apfelbach R, Joerges J, Malaka R. Bioelectronic noses: a status report—part 1. Biosens Bioelectron. 1998;13(3–4):479–93.CrossRefPubMedGoogle Scholar
  10. 10.
    Ziegler C, Göpel W, Hämmerle H, Hatt H, Jung G, Laxhuber L, Schmidt HL, Schütz S, Vögtle F, Zell A. Bioelectronic noses: a status report. Part II Biosens Bioelectron. 1998;13(5):539–71.CrossRefPubMedGoogle Scholar
  11. 11.
    Cablk ME, Sagebiel JC, Heaton JS, Valentin C. Olfaction-based detection distance: a quantitative analysis of how far away dogs recognize tortoise odor and follow it to source. Sensors. 2008;8(4):2208–22.PubMedCentralCrossRefGoogle Scholar
  12. 12.
    Schmidt PL. Companion animals as sentinels for public health. Vet Clin North Am Small Anim Pract. 2009;39(2):241–250.Google Scholar
  13. 13.
    Song HG, Kwon JY, Han HS, Bae YC, Moon C. First contact to odors: our current knowledge about odorant receptors. Sensors. 2008;8(10):6303–20.PubMedCentralCrossRefGoogle Scholar
  14. 14.
    Gomila G, Casuso I, Errachid A, Ruiz O, Pajot E, Minic J, Gorojankina T, Persuy MA, Aioun J, Salesse R, Bausells J, Villanueva G, Rius G, Hou Y, Jaffrezic N, Pennetta C, Alfinito E, Akimov V, Reggiani L, Ferrari G, Fumagalli L, Sampietro M, Samitier J. Advances in the production, immobilization, and electrical characterization of olfactory receptors for olfactory nanobiosensor development. Sens Actuators B Chem. 2006;116(1–2):66–71.Google Scholar
  15. 15.
    Lee SH, Song HS, Oh EH, Park TH. Olfactory biosensor using nanotubes combined with nanovesicles containing human olfactory receptors on their surface. J Biotechnol. 2010;150S42–S42.Google Scholar
  16. 16.
    Oh EH, Song HS, Park TH. Recent advances in electronic and bioelectronic noses and their biomedical applications. Enzyme Microb Technol. 2011;48(6–7):427–37.CrossRefPubMedGoogle Scholar
  17. 17.
    Wang P, Liu QJ, Xu Y, Cai H, Li Y. Olfactory and taste cell sensor and its applications in biomedicine. Sens Actuators A Phys. 2007;139(1–2):131–138.Google Scholar
  18. 18.
    Spehr M, Munger SD. Olfactory receptors: g protein-coupled receptors and beyond. J Neurochem. 2009;109(6):1570–83.PubMedCentralCrossRefPubMedGoogle Scholar
  19. 19.
    Sankaran S, Khot LR, Panigrahi S. Biology and applications of olfactory sensing system: a review. Sens Actuators B Chem. 2012;171–172:1–17.CrossRefGoogle Scholar
  20. 20.
    Du L, Wu C, Liu Q, Huang L, Wang P. Recent advances in olfactory receptor-basedbiosensors. Biosens Bioelectron. 2013;42570–580.Google Scholar
  21. 21.
    Wu TZ. A piezoelectric biosensor as an olfactory receptor for odour detection: electronic nose. Biosens Bioelectron. 1999;14(1):9–18.CrossRefPubMedGoogle Scholar
  22. 22.
    Huotari MJ. Biosensing by insect olfactory receptor neurons. Sens Actuators B Chem. 2000;71(3):212–222.Google Scholar
  23. 23.
    Liu QJ, Cai H, Xu Y, Li Y, Li R, Wang P. Olfactory cell-based biosensor: a first step towards a neurochip of bioelectronic nose. Biosens Bioelectron. 2006;22(2):318–22.CrossRefPubMedGoogle Scholar
  24. 24.
    Wu CS, Chen PH, Yu H, Liu QJ, Zong XL, Cai H, Wang P. A novel biomimetic olfactory-based biosensor for single olfactory sensory neuron monitoring. Biosens Bioelectron. 2009;24(5):1498–502.CrossRefPubMedGoogle Scholar
  25. 25.
    Kim TH, Lee SH, Lee J, Song HS, Oh EH, Park TH, Hong S. Single-carbon-atomic-resolution detection of odorant molecules using a human olfactory receptor-based bioelectronic nose. Adv Mater. 2009;21(1):91–4.CrossRefGoogle Scholar
  26. 26.
    Wu CS, Du LP, Wang D, Wang L, Zhao LH, Wang P. A novel surface acoustic wave-based biosensor for highly sensitive functional assays of olfactory receptors. Biochem Biophys Res Commun. 2011;407(1):18–22.CrossRefPubMedGoogle Scholar
  27. 27.
    Benilova IV, Vidic JM, Pajot-Augy E, Soldatkin AP, Martelet C, Jafftezic-Renault N. Electrochemical study of human olfactory receptor OR 17-40 stimulation by odorants in solution. Mater Sci Eng C. 2008;28(5–6):633–9.CrossRefGoogle Scholar
  28. 28.
    Hou YX, Jaffrezic-Renault N, Martelet C, Zhang AD, Minic-Vidic J, Gorojankina T, Persuy MA, Pajot-Augy E, Salesse R, Akimov V, Reggiani L, Pennetta C, Alfinito E, Ruiz O, Gomila G, Samitier J, Errachid A. A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor 17. Biosens Bioelectron. 2007;22(7):1550–5.CrossRefPubMedGoogle Scholar
  29. 29.
    Marrakchi M, Vidic J, Jaffrezic-Renault N, Martelet C, Pajot-Augy E. A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40. Eur Biophys J. 2007;36(8):1015–8.CrossRefPubMedGoogle Scholar
  30. 30.
    Vidic J, Pla-Roca M, Grosclaude J, Persuy MA, Monnerie R, Caballero D, Errachid A, Hou YX, Jaffrezic-Renault N, Salesse R, Pajot-Augy E, Samitier J. Gold surface functionalization and patterning for specific immobilization of olfactory receptors carried by nanosomes. Anal Chem. 2007;79(9):3280–90.CrossRefPubMedGoogle Scholar
  31. 31.
    Cook BL, Ernberg KE, Chung H, Zhang S. Study of a synthetic human olfactory receptor I7-4 expression and purification from an inducible mammalian cell line. PloS One. 2008;3(8):e2920.Google Scholar
  32. 32.
    Leck KJ, Zhang SG, Hauser CAE. Study of bioengineered zebra fish olfactory receptor 131–2: receptor purification and secondary structure analysis. Plos One. 2010; 5(11).Google Scholar
  33. 33.
    Katzen F, Chang G, Kudlicki W. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 2005;23(3):150–156.Google Scholar
  34. 34.
    Kaiser L, Graveland-Bikker J, Steuerwald D, Vanberghem M, Herlihy K, Zhang S. Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses. Proc Natl Acad Sci U. S. A. 2008;105(41):15726–15731.Google Scholar
  35. 35.
    Sankaran S, Panigrahi S, Mallik S. Olfactory receptor based piezoelectric biosensors for detection of alcohols related to food safety applications. Sens Actuators B Chem. 2011;155(1):8–18.Google Scholar
  36. 36.
    Sankaran S, Panigrahi S, Mallik S. Odorant binding protein based biomimetic sensors for detection of alcohols associated with Salmonella contamination in packaged beef. Biosens Bioelectron. 2011;26(7):3103–9.CrossRefPubMedGoogle Scholar
  37. 37.
    Du L, Wu C, Peng H, Zou L, Zhao L, Huang L, Wang P. Piezoelectric olfactory receptor biosensor prepared by aptamer-assisted immobilization. Sens Actuators B Chem. 2013;187481–487.Google Scholar
  38. 38.
    Wang X, Corin K, Baaske P, Wienken CJ, Jerabek-Willemsen M, Duhr S, Braun D, Zhang S. Peptide surfactants for cell-free production of functional G protein-coupled receptors. Proc Natl Acad Sci. 2011;108(22):9049–54.PubMedCentralCrossRefPubMedGoogle Scholar
  39. 39.
    Schöning MJ, Poghossian A. Bio FEDs (field-effect devices): state-of-the-art and new directions. Electroanalysis. 2006;18(19–20):1893–900.CrossRefGoogle Scholar
  40. 40.
    Yoon H, Lee SH, Kwon OS, Song HS, Oh EH, Park TH, Jang J. Polypyrrole nanotubes conjugated with human olfactory receptors: high-performance transducers for FET-type bioelectronic noses. Angew Chem Int Ed. 2009;48(15):2755–8.CrossRefGoogle Scholar
  41. 41.
    Alfinito E, Millithaler JF, Pennetta C, Reggiani L. A single protein based nanobiosensor for odorant recognition. Microelectro J. 2010;41(11):718–22.CrossRefGoogle Scholar
  42. 42.
    Alfinito E, Millithaler JF, Reggiani L, Zine N, Jaffrezic-Renault N. Human olfactory receptor 17-40 as an active part of a nanobiosensor: a microscopic investigation of its electrical properties. Rsc Advances. 2011;1(1):123–7.CrossRefGoogle Scholar
  43. 43.
    Ko HJ, Park TH. Piezoelectric olfactory biosensor: ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system. Biosens Bioelectron. 2005;20(7):1327–32.CrossRefPubMedGoogle Scholar
  44. 44.
    Sung JH, Ko HJ, Park TH. Piezoelectric biosensor using olfactory receptor protein expressed in Escherichia coli. Biosens Bioelectron. 2006;21(10):1981–6.CrossRefPubMedGoogle Scholar
  45. 45.
    Lu HH, Rao YK, Wu TZ, Tzeng YM. Direct characterization and quantification of volatile organic compounds by piezoelectric module chips sensor. Sens Actuators B Chem. 2009;137(2):741–6.CrossRefGoogle Scholar
  46. 46.
    Früh V, Ijzerman AP, Siegal G. How to catch a membrane protein in action: a review of functional membrane protein immobilization strategies and their applications. Chem. Rev. 2011;111640–656.Google Scholar
  47. 47.
    Casuso I, Pla-Roca M, Gomila G, Samitier J, Minic J, Persuy MA, Salesse R, Pajot-Augy E. Immobilization of olfactory receptors onto gold electrodes for electrical biosensor. Mat Sci Eng C. 2008;28(5–6):686–91.CrossRefGoogle Scholar
  48. 48.
    Hou YX, Helali S, Zhang AD, Jaffrezic-Renault N, Martelet C, Minic J, Gorojankina T, Persuy MA, Pajot-Augy E, Salesse R, Bessueille F, Samitier J, Errachid A, Akimov V, Reggiani L, Pennetta C, Alfinito E. Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy. Biosens Bioelectron. 2006;21(7):1393–402.CrossRefPubMedGoogle Scholar
  49. 49.
    Vidic JM, Grosclaude J, Persuy MA, Aioun J, Salesse R, Pajot-Augy E. Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose. Lab Chip. 2006;6(8):1026–32.CrossRefPubMedGoogle Scholar
  50. 50.
    Suska A, Ibanez AB, Lundstrom I, Berghard A. G protein-coupled receptor mediated trimethylamine sensing. Biosens Bioelectron. 2009;25(4):715–720.Google Scholar
  51. 51.
    Kim TH, Lee BY, Jaworski J, Yokoyama K, Chung W-J, Wang E, Hong S, Majumdar A, Lee S-W. Selective and sensitive TNT sensors using biomimetic polydiacetylene-coated CNT-FETs. ACS Nano. 2011;5(4):2824–30.CrossRefPubMedGoogle Scholar
  52. 52.
    Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. Nat Chem Biol. 2007;3(6):325–30.CrossRefPubMedGoogle Scholar
  53. 53.
    Corcelli A, Lobasso S, Lopalco P, Dibattista M, Araneda R, Peterlin Z, Firestein S. Detection of explosives by olfactory sensory neurons. J Hazard Mater. 2010;175(1–3):1096–100.CrossRefPubMedGoogle Scholar
  54. 54.
    Marshall B, Warr CG, de Bruyne M. Detection of volatile indicators of illicit substances by the olfactory receptors of drosophila melanogaster. Chem Senses. 2010;35(7):613–25.PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Science Press, Beijing and Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Zhejiang UniversityHangzhouChina

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